Mol. Cells 2011; 32(5): 477-482
Published online October 21, 2011
https://doi.org/10.1007/s10059-011-0168-6
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *Correspondence: seunglee@snu.ac.kr
Sac1 phosphoinositide (PI) phosphatases are important regulators of PtdIns(4)P turnover at the ER, Golgi, and plasma membrane (PM) and are involved in diverse cellular processes including cytoskeletal organization and vesicular trafficking. Here, we present evidence that Sac1 regulates axon guidance in the embryonic CNS of Drosophila. Sac1 is expressed on three longitudinal axon tracts that are defined by the cell adhesion molecule Fasciclin II (Fas II). Mutations in the sac1 gene cause ectopic midline crossing of Fas II-positive axon tracts. This phenotype is rescued by neuronal expression of wild-type Sac1 but not by a catalytically-inactive mutant. Finally, sac1 displays dosage-sensitive genetic interactions with mutations in the genes that encode the midline repellent Slit and its axonal receptor Robo. Taken together, our results suggest that Sac1-mediated regulation of PIs is critical for Slit/ Robo-dependent axon repulsion at the CNS midline.
Keywords Drosophila, midline axon guidance, phosphoinositide phosphatase, Sac1
Mol. Cells 2011; 32(5): 477-482
Published online November 30, 2011 https://doi.org/10.1007/s10059-011-0168-6
Copyright © The Korean Society for Molecular and Cellular Biology.
Seongsoo Lee1,5, Sungdae Kim2,5, Minyeop Nahm1, Euijae Kim1, Tai-Il Kim3, Jin Ho Yoon4, and Seungbok Lee1,2,*
1Department of Cell and Developmental Biology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-749, Korea, 2Interdisciplinary Graduate Program in Genetic Engineering, Seoul National University, Seoul 151-742, Korea, 3Department of Periodontology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-749, Korea, 4Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women’s University, Seoul 142-732, Korea, 5These authors contributed equally to this work.
Correspondence to:*Correspondence: seunglee@snu.ac.kr
Sac1 phosphoinositide (PI) phosphatases are important regulators of PtdIns(4)P turnover at the ER, Golgi, and plasma membrane (PM) and are involved in diverse cellular processes including cytoskeletal organization and vesicular trafficking. Here, we present evidence that Sac1 regulates axon guidance in the embryonic CNS of Drosophila. Sac1 is expressed on three longitudinal axon tracts that are defined by the cell adhesion molecule Fasciclin II (Fas II). Mutations in the sac1 gene cause ectopic midline crossing of Fas II-positive axon tracts. This phenotype is rescued by neuronal expression of wild-type Sac1 but not by a catalytically-inactive mutant. Finally, sac1 displays dosage-sensitive genetic interactions with mutations in the genes that encode the midline repellent Slit and its axonal receptor Robo. Taken together, our results suggest that Sac1-mediated regulation of PIs is critical for Slit/ Robo-dependent axon repulsion at the CNS midline.
Keywords: Drosophila, midline axon guidance, phosphoinositide phosphatase, Sac1
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