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Mol. Cells 2001; 12(2): 239-243

Published online October 31, 2001

© The Korean Society for Molecular and Cellular Biology

Differential and Cell-Type Specific Microheterogeneity of HighMannose-Type Asn-linked Oligosaccharides of Human Transferrin Receptor

Ki-Young Lee, Su-Il Do

Abstract

The structural analysis of high mannose-type Asn-linked (N-linked) oligosaccharides of the human trans-ferrin receptor (hTR) from D-[2-3H]mannose meta-bolic-radiolabeled human cells - A431, K562, BeWo, and HL60 - was investigated. The radiolabeled hTR glycopeptides were prepared and fractionated by a lectin chromatography of Concanavalin A-Sepharose. The composition analysis of hTR glycopeptides re-vealed that Con A-I contains both mannose and fucose, whereas Con A-III has mannose exclusively. The Con A-III glycopeptides were treated with Endo H. The released oligosaccharides were charge-fractionated by QAE-Sephadex. The neutral oligosaccharides were further size-fractionated by an amine absorption high performance liquid chromatography (HPLC). Our results demonstrate that the high mannose-type oligo-saccharides of hTR ranged in size from Man5-R to Man9-R with cell-type specific patterns. A relative amount of each component was found to be differen-tially heterogeneous among the four different human cell lines.

Keywords Hum, High Mannose-Type Asn-linked Oligosaccharides

Article

Research Article

Mol. Cells 2001; 12(2): 239-243

Published online October 31, 2001

Copyright © The Korean Society for Molecular and Cellular Biology.

Differential and Cell-Type Specific Microheterogeneity of HighMannose-Type Asn-linked Oligosaccharides of Human Transferrin Receptor

Ki-Young Lee, Su-Il Do

Abstract

The structural analysis of high mannose-type Asn-linked (N-linked) oligosaccharides of the human trans-ferrin receptor (hTR) from D-[2-3H]mannose meta-bolic-radiolabeled human cells - A431, K562, BeWo, and HL60 - was investigated. The radiolabeled hTR glycopeptides were prepared and fractionated by a lectin chromatography of Concanavalin A-Sepharose. The composition analysis of hTR glycopeptides re-vealed that Con A-I contains both mannose and fucose, whereas Con A-III has mannose exclusively. The Con A-III glycopeptides were treated with Endo H. The released oligosaccharides were charge-fractionated by QAE-Sephadex. The neutral oligosaccharides were further size-fractionated by an amine absorption high performance liquid chromatography (HPLC). Our results demonstrate that the high mannose-type oligo-saccharides of hTR ranged in size from Man5-R to Man9-R with cell-type specific patterns. A relative amount of each component was found to be differen-tially heterogeneous among the four different human cell lines.

Keywords: Hum, High Mannose-Type Asn-linked Oligosaccharides

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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