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Mol. Cells 1996; 6(5): 563-570

Published online October 31, 1996

© The Korean Society for Molecular and Cellular Biology

Characterization of 475 Expressed Sequence Tags Generated from Root cDNA Clones of Brassica napus by Single-pass Sequencing

June Myoung Kwak Sun A Kim Moon Soo Soh, Yu Shin Park Eui Seok Shin
Yong Jin Kim Il Chan Kwun and Hong Gil Nam

Abstract

As a part of our efforts to provide a molecular genetic resource of Brassica plants, we have generated 475 expressed sequence tags (ESTs) from randomly selected 412 root cDNA clones of Brassica napus. These ESTs were generated from two types of cDNA library; one primed with oligo(dT) but with partial deletions from the ends of the cDNA molecules and the other primed with random hexamer. Of the 412 clones, 333 clones (79.3 %) provided non-redundant ESTs. A similarity search of these ESTs against the NCBI non-redundant protein database revealed that 139 ESTs (29.3%) are significantly similar to the protein coding sequences in the database. These include 42 ESTs related to the genes from other kingdoms, showing that the EST approach is effective in identifying previously unknown plant genes in a large scale. Functional categorization of the databasematched ESTs showed that defense- or stress-related genes as well as genes involved in the primary metabolic pathways and in translation of mRNAs are most abundantly represented. We also discuss the comparison analysis of the characteristics of the ESTs generated from the different types of cDNA library.

Article

Research Article

Mol. Cells 1996; 6(5): 563-570

Published online October 31, 1996

Copyright © The Korean Society for Molecular and Cellular Biology.

Characterization of 475 Expressed Sequence Tags Generated from Root cDNA Clones of Brassica napus by Single-pass Sequencing

June Myoung Kwak Sun A Kim Moon Soo Soh, Yu Shin Park Eui Seok Shin
Yong Jin Kim Il Chan Kwun and Hong Gil Nam

Abstract

As a part of our efforts to provide a molecular genetic resource of Brassica plants, we have generated 475 expressed sequence tags (ESTs) from randomly selected 412 root cDNA clones of Brassica napus. These ESTs were generated from two types of cDNA library; one primed with oligo(dT) but with partial deletions from the ends of the cDNA molecules and the other primed with random hexamer. Of the 412 clones, 333 clones (79.3 %) provided non-redundant ESTs. A similarity search of these ESTs against the NCBI non-redundant protein database revealed that 139 ESTs (29.3%) are significantly similar to the protein coding sequences in the database. These include 42 ESTs related to the genes from other kingdoms, showing that the EST approach is effective in identifying previously unknown plant genes in a large scale. Functional categorization of the databasematched ESTs showed that defense- or stress-related genes as well as genes involved in the primary metabolic pathways and in translation of mRNAs are most abundantly represented. We also discuss the comparison analysis of the characteristics of the ESTs generated from the different types of cDNA library.

Mol. Cells
Jul 31, 2022 Vol.45 No.7, pp. 435~512
COVER PICTURE
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into mesodermal lineages like adipogenic, osteogenic, and chondrogenic. Alcian blue-positive extracellular matrix secreted by chondrocytes in the lacuna confirmed the chondrogenic differentiation of MSCs (Bashyal et al., pp. 479-494).

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