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Mol. Cells 2013; 36(3): 273-277

Published online September 17, 2014

https://doi.org/10.1007/s10059-013-0226-3

© The Korean Society for Molecular and Cellular Biology

Selective Induction of P2Y14 Receptor by RANKL Promotes Osteoclast Formation

Seung Ah Lee, Jin Hee Park, and Soo Young Lee

Department of Life Science and the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea

Received: August 14, 2013; Accepted: August 26, 2013

Abstract

The purinergic receptor P2Y, G protein coupled, 14 (P2Y14) receptor for UDP-glucose and other UDP-sugars has been implicated in the regulation of the stem cell compartment as well as neuroimmune function. However, the role of P2Y14 in osteoclast formation is completely unknown. We found that RANKL selectively induced P2Y14 among seven mammalian P2Y receptors when analysed at both the mRNA and protein level, but inhibitors of the mitogen-activated protein (MAP) kinase pathway suppressed induction of P2Y14 proteins. Extracellular addition of UDP-su-gars such as UDP-glucose, UDP-galactose, UDP-glucuro-nic acid, and UDP-N-acetyl glucosamine promoted RANKL- induced osteoclastogenesis, while P2Y14 downregulation by RNA interference inhibited osteoclast formation. Taken together, these results suggest that P2Y14 may act as the receptor for UDP-sugars in osteoclast precusors and may regulate RANKL-induced osteoclastogenesis.

Keywords osteoclastogenesis, P2Y14 receptor, RANKL, UDP-sugars

Article

Research Article

Mol. Cells 2013; 36(3): 273-277

Published online September 30, 2013 https://doi.org/10.1007/s10059-013-0226-3

Copyright © The Korean Society for Molecular and Cellular Biology.

Selective Induction of P2Y14 Receptor by RANKL Promotes Osteoclast Formation

Seung Ah Lee, Jin Hee Park, and Soo Young Lee

Department of Life Science and the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea

Received: August 14, 2013; Accepted: August 26, 2013

Abstract

The purinergic receptor P2Y, G protein coupled, 14 (P2Y14) receptor for UDP-glucose and other UDP-sugars has been implicated in the regulation of the stem cell compartment as well as neuroimmune function. However, the role of P2Y14 in osteoclast formation is completely unknown. We found that RANKL selectively induced P2Y14 among seven mammalian P2Y receptors when analysed at both the mRNA and protein level, but inhibitors of the mitogen-activated protein (MAP) kinase pathway suppressed induction of P2Y14 proteins. Extracellular addition of UDP-su-gars such as UDP-glucose, UDP-galactose, UDP-glucuro-nic acid, and UDP-N-acetyl glucosamine promoted RANKL- induced osteoclastogenesis, while P2Y14 downregulation by RNA interference inhibited osteoclast formation. Taken together, these results suggest that P2Y14 may act as the receptor for UDP-sugars in osteoclast precusors and may regulate RANKL-induced osteoclastogenesis.

Keywords: osteoclastogenesis, P2Y14 receptor, RANKL, UDP-sugars

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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