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Mol. Cells 2013; 36(3): 227-234

Published online September 30, 2013

https://doi.org/10.1007/s10059-013-0082-1

© The Korean Society for Molecular and Cellular Biology

Regulation of Transcription from Two ssrS Promoters in 6S RNA Biogenesis

Ji Young Lee, Hongmarn Park, Geunu Bak, Kwang-sun Kim, and Younghoon Lee

Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea, 1Superbacteria Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea

Received: March 11, 2013; Revised: June 24, 2013; Accepted: June 24, 2013

Abstract

ssrS-encoded 6S RNA is an abundant noncoding RNA that binds σ70-RNA polymerase and regulates expression at a subset of promoters in Escherichia coli. It is transcribed from two tandem promoters, ssrS P1 and ssrS P2. Regulation of transcription from two ssrS promoters in 6S RNA biogenesis was examined. Both P1 and P2 were growth phase-dependently regulated. Depletion of 6S RNA had no effect on growth-phase-dependent transcription from either promoter, whereas overexpression of 6S RNA increased P1 transcription and decreased P2 transcription,
suggesting that transcription from P1 and P2 is subject to feedback activation and feedback inhibition, respectively. This feedback regulation disappeared in Δfis strains, supporting involvement of Fis in this process. The differential feedback regulation may provide a means for maintaining appropriate cellular concentrations of 6S RNA.

Keywords 6S RNA, feedback regulation, Fis, promoter, sigma factor

Article

Research Article

Mol. Cells 2013; 36(3): 227-234

Published online September 30, 2013 https://doi.org/10.1007/s10059-013-0082-1

Copyright © The Korean Society for Molecular and Cellular Biology.

Regulation of Transcription from Two ssrS Promoters in 6S RNA Biogenesis

Ji Young Lee, Hongmarn Park, Geunu Bak, Kwang-sun Kim, and Younghoon Lee

Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea, 1Superbacteria Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea

Received: March 11, 2013; Revised: June 24, 2013; Accepted: June 24, 2013

Abstract

ssrS-encoded 6S RNA is an abundant noncoding RNA that binds σ70-RNA polymerase and regulates expression at a subset of promoters in Escherichia coli. It is transcribed from two tandem promoters, ssrS P1 and ssrS P2. Regulation of transcription from two ssrS promoters in 6S RNA biogenesis was examined. Both P1 and P2 were growth phase-dependently regulated. Depletion of 6S RNA had no effect on growth-phase-dependent transcription from either promoter, whereas overexpression of 6S RNA increased P1 transcription and decreased P2 transcription,
suggesting that transcription from P1 and P2 is subject to feedback activation and feedback inhibition, respectively. This feedback regulation disappeared in Δfis strains, supporting involvement of Fis in this process. The differential feedback regulation may provide a means for maintaining appropriate cellular concentrations of 6S RNA.

Keywords: 6S RNA, feedback regulation, Fis, promoter, sigma factor

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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