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Mol. Cells 2013; 36(3): 227-234
Published online July 16, 2013
https://doi.org/10.1007/s10059-013-0082-1
© The Korean Society for Molecular and Cellular Biology
Regulation of Transcription from Two ssrS Promoters in 6S RNA Biogenesis
ssrS-encoded 6S RNA is an abundant noncoding RNA that binds σ70-RNA polymerase and regulates expression at a subset of promoters in Escherichia coli. It is transcribed from two tandem promoters, ssrS P1 and ssrS P2. Regulation of transcription from two ssrS promoters in 6S RNA biogenesis was examined. Both P1 and P2 were growth phase-dependently regulated. Depletion of 6S RNA had no effect on growth-phase-dependent transcription from either promoter, whereas overexpression of 6S RNA increased P1 transcription and decreased P2 transcription,
suggesting that transcription from P1 and P2 is subject to feedback activation and feedback inhibition, respectively. This feedback regulation disappeared in Δfis strains, supporting involvement of Fis in this process. The differential feedback regulation may provide a means for maintaining appropriate cellular concentrations of 6S RNA.
Keywords 6S RNA, feedback regulation, Fis, promoter, sigma factor
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Research Article
Mol. Cells 2013; 36(3): 227-234
Published online September 30, 2013 https://doi.org/10.1007/s10059-013-0082-1
Copyright © The Korean Society for Molecular and Cellular Biology.
Regulation of Transcription from Two ssrS Promoters in 6S RNA Biogenesis
Ji Young Lee, Hongmarn Park, Geunu Bak, Kwang-sun Kim, and Younghoon Lee
Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea, 1Superbacteria Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea
Abstract
ssrS-encoded 6S RNA is an abundant noncoding RNA that binds σ70-RNA polymerase and regulates expression at a subset of promoters in Escherichia coli. It is transcribed from two tandem promoters, ssrS P1 and ssrS P2. Regulation of transcription from two ssrS promoters in 6S RNA biogenesis was examined. Both P1 and P2 were growth phase-dependently regulated. Depletion of 6S RNA had no effect on growth-phase-dependent transcription from either promoter, whereas overexpression of 6S RNA increased P1 transcription and decreased P2 transcription,
suggesting that transcription from P1 and P2 is subject to feedback activation and feedback inhibition, respectively. This feedback regulation disappeared in Δfis strains, supporting involvement of Fis in this process. The differential feedback regulation may provide a means for maintaining appropriate cellular concentrations of 6S RNA.
Keywords: 6S RNA, feedback regulation, Fis, promoter, sigma factor
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