TOP

Research Article

Split Viewer

Mol. Cells 2013; 35(6): 526-532

Published online May 16, 2013

https://doi.org/10.1007/s10059-013-0038-5

© The Korean Society for Molecular and Cellular Biology

Curcumin Induces Apoptosis in Human Colorectal Carcinoma (HCT-15) Cells by Regulating Expression of Prp4 and p53

Adeeb Shehzad, Jaetae Lee, Tae-Lin Huh, and Young Sup Lee

School of Life Sciences, College of Natural Sciences, Kyungpook National University, 1Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu 700-721, Korea

Received: February 4, 2013; Revised: March 13, 2013; Accepted: March 28, 2013

Abstract

Curcumin (diferuloylmethane), the yellow pigment of turmeric, is one of the most commonly used and exten-sively studied phytochemicals due to its pleiotropic effects in several human cancers. In the current study, the therapeutic efficacy of curcumin was investigated in human colorectal carcinoma HCT-15 cells. Curcumin inhibited HCT-15 cells proliferation and induced apoptosis in a dose- and time-dependent manner. Hoechst 33342 and DCFHDA staining revealed morpho-logical and biochemical features of apoptosis as well as ROS generation in HCT-15 cells treated with 30 and 50 ?M curcumin. Over-expression of pre-mRNA processing factor 4B (Prp4B) and p53 mutations have been reported as hallmarks of cancer cells. Western blot analysis revealed that curcumin treatment activated caspase-3 and decreased expression of p53 and Prp4B in a time-dependent manner. Transfection of HCT-15 cells with Prp4B clone perturbed the growth inhibition induced by 30 ?M curcumin. Fractionation of cells revealed increased accumulation of Prp4B in the nucleus, following its translocation from the cytoplasm. To further evaluate the underlying mechanism and survival effect of Prp4B, we generated siRNA-Prp4B HCT15 clones. Knockdown of Prp4B with siRNA diminished the protective effects of Prp4B against curcumin-induced apoptosis. These results suggest a possible underlying molecular mecha-nism in which Prp4B over-expression and activity are closely associated with the survival and regulation of apoptotic events in human colon cancer HCT-15 cells.

Keywords apoptosis, colorectal cancer, curcumin, Prp4B

Article

Research Article

Mol. Cells 2013; 35(6): 526-532

Published online June 30, 2013 https://doi.org/10.1007/s10059-013-0038-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Curcumin Induces Apoptosis in Human Colorectal Carcinoma (HCT-15) Cells by Regulating Expression of Prp4 and p53

Adeeb Shehzad, Jaetae Lee, Tae-Lin Huh, and Young Sup Lee

School of Life Sciences, College of Natural Sciences, Kyungpook National University, 1Department of Nuclear Medicine, Kyungpook National University Hospital, Daegu 700-721, Korea

Received: February 4, 2013; Revised: March 13, 2013; Accepted: March 28, 2013

Abstract

Curcumin (diferuloylmethane), the yellow pigment of turmeric, is one of the most commonly used and exten-sively studied phytochemicals due to its pleiotropic effects in several human cancers. In the current study, the therapeutic efficacy of curcumin was investigated in human colorectal carcinoma HCT-15 cells. Curcumin inhibited HCT-15 cells proliferation and induced apoptosis in a dose- and time-dependent manner. Hoechst 33342 and DCFHDA staining revealed morpho-logical and biochemical features of apoptosis as well as ROS generation in HCT-15 cells treated with 30 and 50 ?M curcumin. Over-expression of pre-mRNA processing factor 4B (Prp4B) and p53 mutations have been reported as hallmarks of cancer cells. Western blot analysis revealed that curcumin treatment activated caspase-3 and decreased expression of p53 and Prp4B in a time-dependent manner. Transfection of HCT-15 cells with Prp4B clone perturbed the growth inhibition induced by 30 ?M curcumin. Fractionation of cells revealed increased accumulation of Prp4B in the nucleus, following its translocation from the cytoplasm. To further evaluate the underlying mechanism and survival effect of Prp4B, we generated siRNA-Prp4B HCT15 clones. Knockdown of Prp4B with siRNA diminished the protective effects of Prp4B against curcumin-induced apoptosis. These results suggest a possible underlying molecular mecha-nism in which Prp4B over-expression and activity are closely associated with the survival and regulation of apoptotic events in human colon cancer HCT-15 cells.

Keywords: apoptosis, colorectal cancer, curcumin, Prp4B

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download