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Mol. Cells 2013; 35(6): 481-488

Published online June 30, 2013

https://doi.org/10.1007/s10059-013-2130-2

© The Korean Society for Molecular and Cellular Biology

Requirement of Vesicle-Associated Membrane Protein 721 and 722 for Sustained Growth during Immune Responses in Arabidopsis

Hye Sup Yun, Mark Kwaaitaal, Naohiro Kato, Changhyun Yi, Sohyeon Park, Masa H. Sato, Paul Schulze-Lefert, and Chian Kwon

1Max-Planck-Institut f?r Z?chtungsforschung, Deparment of Plant Microbe Interactions, D-50829, K?ln, Germany, 2Biological Sciences Major, College of Biological Science and Biotechnology, Konkuk University, Seoul 143-701, Korea, 3Department of Biological Sciences, Louisiana State University, LA 70803-1715, USA, 4Department of Molecular Biology, Dankook University, Yongin 448-701, Korea, 5Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522, Japan, 6Department of Integrated Molecular Sciences, Dankook University, Yongin 448-701, Korea, 7Present address: Department of Agriculture and Ecology Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark

Received: April 30, 2013; Revised: March 21, 2013; Accepted: March 28, 2013

Abstract

Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFPVAMP722 to and from the plasma membrane in nonstimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunityinvolved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cuedependent cargo molecules.

Keywords plant growth, plant immune responses, PM syntaxins, secretion, VAMP721/722

Article

Research Article

Mol. Cells 2013; 35(6): 481-488

Published online June 30, 2013 https://doi.org/10.1007/s10059-013-2130-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Requirement of Vesicle-Associated Membrane Protein 721 and 722 for Sustained Growth during Immune Responses in Arabidopsis

Hye Sup Yun, Mark Kwaaitaal, Naohiro Kato, Changhyun Yi, Sohyeon Park, Masa H. Sato, Paul Schulze-Lefert, and Chian Kwon

1Max-Planck-Institut f?r Z?chtungsforschung, Deparment of Plant Microbe Interactions, D-50829, K?ln, Germany, 2Biological Sciences Major, College of Biological Science and Biotechnology, Konkuk University, Seoul 143-701, Korea, 3Department of Biological Sciences, Louisiana State University, LA 70803-1715, USA, 4Department of Molecular Biology, Dankook University, Yongin 448-701, Korea, 5Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522, Japan, 6Department of Integrated Molecular Sciences, Dankook University, Yongin 448-701, Korea, 7Present address: Department of Agriculture and Ecology Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark

Received: April 30, 2013; Revised: March 21, 2013; Accepted: March 28, 2013

Abstract

Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFPVAMP722 to and from the plasma membrane in nonstimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunityinvolved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cuedependent cargo molecules.

Keywords: plant growth, plant immune responses, PM syntaxins, secretion, VAMP721/722

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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