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Mol. Cells 2013; 35(4): 327-334

Published online April 30, 2013

https://doi.org/10.1007/s10059-013-2317-6

© The Korean Society for Molecular and Cellular Biology

Differential Requirement of Oryza sativa RAR1 in Immune Receptor-Mediated Resistance of Rice to Magnaporthe oryzae

Min-Young Song, Chi-Yeol Kim, Muho Han, Hak-Seung Ryu, Sang-Kyu Lee, Li Sun, Zuhua He, Young-Su Seo, Patrick Canal, Pamela C. Ronald , and Jong-Seong Jeon

1Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea, 2National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China, 3Department of Microbiology, Pusan National University, Busan 609-735, Korea, 4Department of Plant Pathology and the Genome Center, University of California, Davis, CA 95616, USA, 5These authors contributed equally to this work.

Received: December 5, 2013; Revised: February 28, 2013; Accepted: February 28, 2013

Abstract

The required for Mla12 resistance (RAR1) protein is essential for the plant immune response. In rice, a model monocot species, the function of Oryza sativa RAR1 (OsRAR1) has been little explored. In our current study, we characterized the response of a rice osrar1 T-DNA insertion mutant to infection by Magnaporthe oryzae, the causal agent of rice blast disease. osrar1 mutants displayed reduced resistance compared with wild type rice when inoculated with the normally virulent M. oryzae isolate PO6-6, indicating that OsRAR1 is required for an immune response to this pathogen. We also investigated the function of OsRAR1 in the resistance mechanism mediated by the immune receptor genes Pib and Pi5 that encode nucleotide binding-leucine rich repeat (NB-LRR) proteins. We inoculated progeny from Pib/osrar1 and Pi5/osrar1 heterozygous plants with the avirulent M. oryzae isolates, race 007 and PO6-6, respectively. We found that only Pib-mediated resistance was compromised by the osrar1 mutation and that the introduction of the OsRAR1 cDNA into Pib/osrar1 rescued Pib-mediated resistance. These results indicate that OsRAR1 is required for Pib-mediated resistance but not Pi5-mediated resistance to M. oryzae.

Keywords immune receptor, NB-LRR, OsRAR1, resistance, rice

Article

Research Article

Mol. Cells 2013; 35(4): 327-334

Published online April 30, 2013 https://doi.org/10.1007/s10059-013-2317-6

Copyright © The Korean Society for Molecular and Cellular Biology.

Differential Requirement of Oryza sativa RAR1 in Immune Receptor-Mediated Resistance of Rice to Magnaporthe oryzae

Min-Young Song, Chi-Yeol Kim, Muho Han, Hak-Seung Ryu, Sang-Kyu Lee, Li Sun, Zuhua He, Young-Su Seo, Patrick Canal, Pamela C. Ronald , and Jong-Seong Jeon

1Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea, 2National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China, 3Department of Microbiology, Pusan National University, Busan 609-735, Korea, 4Department of Plant Pathology and the Genome Center, University of California, Davis, CA 95616, USA, 5These authors contributed equally to this work.

Received: December 5, 2013; Revised: February 28, 2013; Accepted: February 28, 2013

Abstract

The required for Mla12 resistance (RAR1) protein is essential for the plant immune response. In rice, a model monocot species, the function of Oryza sativa RAR1 (OsRAR1) has been little explored. In our current study, we characterized the response of a rice osrar1 T-DNA insertion mutant to infection by Magnaporthe oryzae, the causal agent of rice blast disease. osrar1 mutants displayed reduced resistance compared with wild type rice when inoculated with the normally virulent M. oryzae isolate PO6-6, indicating that OsRAR1 is required for an immune response to this pathogen. We also investigated the function of OsRAR1 in the resistance mechanism mediated by the immune receptor genes Pib and Pi5 that encode nucleotide binding-leucine rich repeat (NB-LRR) proteins. We inoculated progeny from Pib/osrar1 and Pi5/osrar1 heterozygous plants with the avirulent M. oryzae isolates, race 007 and PO6-6, respectively. We found that only Pib-mediated resistance was compromised by the osrar1 mutation and that the introduction of the OsRAR1 cDNA into Pib/osrar1 rescued Pib-mediated resistance. These results indicate that OsRAR1 is required for Pib-mediated resistance but not Pi5-mediated resistance to M. oryzae.

Keywords: immune receptor, NB-LRR, OsRAR1, resistance, rice

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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