Recently, graphene oxide (GO), one of the carbon nanomaterials, has received much attention due to its unique physical and chemical properties and high potential in many research areas including applications as biosensors and drug delivery vehicle. Various GO-based biosensors, largely based on its surface adsorption properties, have been developed not only for the detection of biomolecules including nucleotides and peptides, but also for the realtime monitoring of enzymatic reactions. In this review, we discuss recent advances of GO-based biosensors focusing on a novel assay platform for helicase activity, which was also employed for high-throughput screening to discover selective helicase inhibitors.

Keywords: biosensor, drug screening, graphene oxide, helicase, hepatitis C