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Mol. Cells 2012; 34(3): 279-287

Published online September 30, 2012

https://doi.org/10.1007/s10059-012-0103-5

© The Korean Society for Molecular and Cellular Biology

Rhythmic Oscillation of Histone Acetylation and Methylation at the Arabidopsis Central Clock Loci

Hae-Ryong Song1,2, and Yoo-Sun Noh1,3,*

1School of Biological Sciences, Seoul National University, Seoul 151-747, Korea, 2Biosafety Research Team, Environmental Health Research Department, National Institute of Environmental Research, Incheon 404-708, Korea, 3Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-742, Korea.

Correspondence to : *Correspondence: ysnoh@snu.ac.kr

Received: April 9, 2012; Revised: July 12, 2012; Accepted: July 16, 2012

Abstract

Circadian clock genes are regulated by a transcriptional-translational feedback loop. In Arabidopsis, LATE ELON-GATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSO- CIATED 1 (CCA1) transcripts are highly expressed in the morning. Translated LHY and CCA1 proteins repress the expression of TIMING OF CAB EXPRESSION 1 (TOC1), which peaks in the evening. TOC1 protein induces expression of LHY and CCA1, forming a negative feedback loop which is believed to constitute the oscillatory mechanism of the clock. The rhythmic oscillation of mouse clock genes mPERIOD 1 (mPER1) and mPER2 has been correlated with regular alteration of chromatin structure through histone acetylation/deacetylation. However, little is known about the relationship between the transcriptional activity of Arabidopsis clock genes and their chromatin status. Here, we report that histone H3 acetylation (H3Ac) and H3 lysine 4 tri-methylation (H3K4me3) levels at LHY, CCA1, and TOC1 are positively correlated with the rhythmic transcript levels of these genes, whereas H3K36me2 level shows a negative correlation. Thus, our study suggests rhythmic transcription of Arabidopsis clock genes might be regulated by rhythmic histone modification, and it provides a platform for future identification of clock-control-ling histone modifiers.

Keywords Arabidopsis, chromatin, circadian clock, circadian rhythm, histone modification

Article

Research Article

Mol. Cells 2012; 34(3): 279-287

Published online September 30, 2012 https://doi.org/10.1007/s10059-012-0103-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Rhythmic Oscillation of Histone Acetylation and Methylation at the Arabidopsis Central Clock Loci

Hae-Ryong Song1,2, and Yoo-Sun Noh1,3,*

1School of Biological Sciences, Seoul National University, Seoul 151-747, Korea, 2Biosafety Research Team, Environmental Health Research Department, National Institute of Environmental Research, Incheon 404-708, Korea, 3Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-742, Korea.

Correspondence to:*Correspondence: ysnoh@snu.ac.kr

Received: April 9, 2012; Revised: July 12, 2012; Accepted: July 16, 2012

Abstract

Circadian clock genes are regulated by a transcriptional-translational feedback loop. In Arabidopsis, LATE ELON-GATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSO- CIATED 1 (CCA1) transcripts are highly expressed in the morning. Translated LHY and CCA1 proteins repress the expression of TIMING OF CAB EXPRESSION 1 (TOC1), which peaks in the evening. TOC1 protein induces expression of LHY and CCA1, forming a negative feedback loop which is believed to constitute the oscillatory mechanism of the clock. The rhythmic oscillation of mouse clock genes mPERIOD 1 (mPER1) and mPER2 has been correlated with regular alteration of chromatin structure through histone acetylation/deacetylation. However, little is known about the relationship between the transcriptional activity of Arabidopsis clock genes and their chromatin status. Here, we report that histone H3 acetylation (H3Ac) and H3 lysine 4 tri-methylation (H3K4me3) levels at LHY, CCA1, and TOC1 are positively correlated with the rhythmic transcript levels of these genes, whereas H3K36me2 level shows a negative correlation. Thus, our study suggests rhythmic transcription of Arabidopsis clock genes might be regulated by rhythmic histone modification, and it provides a platform for future identification of clock-control-ling histone modifiers.

Keywords: Arabidopsis, chromatin, circadian clock, circadian rhythm, histone modification

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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