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Mol. Cells 2012; 33(6): 545-551

Published online June 7, 2012

https://doi.org/10.1007/s10059-012-2267-4

© The Korean Society for Molecular and Cellular Biology

MicroRNA-590-5p Regulates Proliferation and Invasion in Human Hepatocellular Carcinoma Cells by Targeting TGF-β RII

Xiaofeng Jiang, Guangyang Xiang, Yezeng Wang, Lei Zhang1, Xuewei Yang, Liangqi Cao, Heping Peng, Ping Xue, and De Chen*

Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Guangzhou Medical College, China, 1Medical School, Tongji University, China

Correspondence to : *Correspondence: drdechen@yahoo.cn

Received: November 23, 2011; Revised: March 24, 2012; Accepted: April 3, 2012

Abstract

MicroRNAs (miRNAs) are regulatory small non-coding RNAs that can regulate gene expression by binding to gene elements, such as the gene promotor 5?UTR, mainly in the 3?UTR of mRNA. One miRNA targets many mRNAs, which can be regulated by many miRNAs, leading to a complex metabolic network. In our study, we found that the expression level of miR-590-5p is higher in the human hepatocellular carcinoma cell line HepG2 than in the normal hepatocellular cell line L02. Downregulation of miR-590-5p inhibited proliferation and invasion of hepatocellular carcinoma cells (HCCs). We also showed that expression of TGF-beta RII, which has been regarded as a regulator of tumor proliferation, invasion, and migration in hepatocellular carcinoma, is regulated by miRNA-590-5p. In addition, miR-590-5p downregulated the expression of TGF- beta RII by targeting the 3?UTR of mRNA. We also found that downregulation of miR-590-5p was associated with an elevation of TGF-beta RII and inhibition of proliferation and invasion in HepG2 cells. Furthermore, overex-pression of miR-590-5p was associated with upregulation of TGF-beta RII and could promote proliferation and invasion in L02 cells. In conclusion, we determined that TGF-beta RII is a novel target of miRNA-590-5p. Thus, the role of TGF-beta RII in regulating proliferation and invasion of human HCCs is controlled by miR-590-5p. In other words, miR-590-5p promotes proliferation and invasion in human HCCs by directly targeting TGF-beta RII.

Keywords HepG2, invasion, L02, miR-590-5p, proliferation, TGF-beta RII

Article

Research Article

Mol. Cells 2012; 33(6): 545-551

Published online June 30, 2012 https://doi.org/10.1007/s10059-012-2267-4

Copyright © The Korean Society for Molecular and Cellular Biology.

MicroRNA-590-5p Regulates Proliferation and Invasion in Human Hepatocellular Carcinoma Cells by Targeting TGF-β RII

Xiaofeng Jiang, Guangyang Xiang, Yezeng Wang, Lei Zhang1, Xuewei Yang, Liangqi Cao, Heping Peng, Ping Xue, and De Chen*

Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Guangzhou Medical College, China, 1Medical School, Tongji University, China

Correspondence to:*Correspondence: drdechen@yahoo.cn

Received: November 23, 2011; Revised: March 24, 2012; Accepted: April 3, 2012

Abstract

MicroRNAs (miRNAs) are regulatory small non-coding RNAs that can regulate gene expression by binding to gene elements, such as the gene promotor 5?UTR, mainly in the 3?UTR of mRNA. One miRNA targets many mRNAs, which can be regulated by many miRNAs, leading to a complex metabolic network. In our study, we found that the expression level of miR-590-5p is higher in the human hepatocellular carcinoma cell line HepG2 than in the normal hepatocellular cell line L02. Downregulation of miR-590-5p inhibited proliferation and invasion of hepatocellular carcinoma cells (HCCs). We also showed that expression of TGF-beta RII, which has been regarded as a regulator of tumor proliferation, invasion, and migration in hepatocellular carcinoma, is regulated by miRNA-590-5p. In addition, miR-590-5p downregulated the expression of TGF- beta RII by targeting the 3?UTR of mRNA. We also found that downregulation of miR-590-5p was associated with an elevation of TGF-beta RII and inhibition of proliferation and invasion in HepG2 cells. Furthermore, overex-pression of miR-590-5p was associated with upregulation of TGF-beta RII and could promote proliferation and invasion in L02 cells. In conclusion, we determined that TGF-beta RII is a novel target of miRNA-590-5p. Thus, the role of TGF-beta RII in regulating proliferation and invasion of human HCCs is controlled by miR-590-5p. In other words, miR-590-5p promotes proliferation and invasion in human HCCs by directly targeting TGF-beta RII.

Keywords: HepG2, invasion, L02, miR-590-5p, proliferation, TGF-beta RII

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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