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Mol. Cells 2012; 33(5): 479-486

Published online May 31, 2012

https://doi.org/10.1007/s10059-012-2266-5

© The Korean Society for Molecular and Cellular Biology

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

Hangeun Kim1, Bong Jun Jung2, Ji Hae Jung2, Joo Yun Kim2, Sung Kyun Chung3, and Dae Kyun Chung2,4,5,*

1Department of Internal Medicine, Saint Louis University, St. Louis, MO 63104, USA, 2School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, Korea, 3Department of Dental Hygiene, Shinheung College, Uijeongbu 480-701, Korea, 4Skin Biotechnology Center, Kyung Hee University, Yongin 449-701, Korea, 5RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea

Correspondence to : *Correspondence: dkchung@khu.ac.kr

Received: November 22, 2011; Revised: March 8, 2012; Accepted: March 8, 2012

Abstract

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-alpha also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-alpha stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

Keywords colon, cytokine, HT-29, inflammation, lipoteichoic acid

Article

Research Article

Mol. Cells 2012; 33(5): 479-486

Published online May 31, 2012 https://doi.org/10.1007/s10059-012-2266-5

Copyright © The Korean Society for Molecular and Cellular Biology.

Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-α-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

Hangeun Kim1, Bong Jun Jung2, Ji Hae Jung2, Joo Yun Kim2, Sung Kyun Chung3, and Dae Kyun Chung2,4,5,*

1Department of Internal Medicine, Saint Louis University, St. Louis, MO 63104, USA, 2School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, Korea, 3Department of Dental Hygiene, Shinheung College, Uijeongbu 480-701, Korea, 4Skin Biotechnology Center, Kyung Hee University, Yongin 449-701, Korea, 5RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Korea

Correspondence to:*Correspondence: dkchung@khu.ac.kr

Received: November 22, 2011; Revised: March 8, 2012; Accepted: March 8, 2012

Abstract

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-alpha also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-alpha stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

Keywords: colon, cytokine, HT-29, inflammation, lipoteichoic acid

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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