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Mol. Cells 2012; 33(2): 135-140

Published online February 29, 2012

https://doi.org/10.1007/s10059-012-2188-2

© The Korean Society for Molecular and Cellular Biology

Overexpression of Arabidopsis Dehydration-Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Jung Eun Hwang1, Chan Ju Lim1, Huan Chen1, Jihyun Je2, Chieun Song2, and Chae Oh Lim1,2,*

1Systems and Synthetic Agrobiotech Center and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea, 2Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University, Jinju 660-701, Korea

Correspondence to : *Correspondence: colim@gnu.ac.kr

Received: September 6, 2011; Revised: December 1, 2011; Accepted: December 2, 2011

Abstract

Dehydration-responsive element-binding proteins (DREBs) regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluo-rescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H2O2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activator-reporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.

Keywords gene expression, signaling, transcription factor, transgenic plant

Article

Research Article

Mol. Cells 2012; 33(2): 135-140

Published online February 29, 2012 https://doi.org/10.1007/s10059-012-2188-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Overexpression of Arabidopsis Dehydration-Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Jung Eun Hwang1, Chan Ju Lim1, Huan Chen1, Jihyun Je2, Chieun Song2, and Chae Oh Lim1,2,*

1Systems and Synthetic Agrobiotech Center and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea, 2Division of Applied Life Science (Brain Korea 21 Program), Graduate School of Gyeongsang National University, Jinju 660-701, Korea

Correspondence to:*Correspondence: colim@gnu.ac.kr

Received: September 6, 2011; Revised: December 1, 2011; Accepted: December 2, 2011

Abstract

Dehydration-responsive element-binding proteins (DREBs) regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluo-rescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H2O2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activator-reporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.

Keywords: gene expression, signaling, transcription factor, transgenic plant

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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