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Mol. Cells 2012; 33(2): 127-133

Published online January 2, 2012

https://doi.org/10.1007/s10059-012-2182-8

© The Korean Society for Molecular and Cellular Biology

Expression Profiling after Induction of Demethylation in MCF-7 Breast Cancer Cells Identifies Involvement of TNF-α Mediated Cancer Pathways

Ju Hee Kim1,3, Seongeun Kang1,3, Tae Woo Kim1, Lihong Yin2, Ran Liu2,*, and Sun Jung Kim1,*

1Department of Life Science, Dongguk University-Seoul, Seoul 100-715, Korea, 2Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China, 3These authors contributed equally to this work.

Correspondence to : *Correspondence: sunjungk@dongguk.edu (SJK); ranliu@seu.edu.cn (RL)

Received: September 2, 2011; Revised: November 18, 2011; Accepted: November 18, 2011

Abstract

Epigenetic methylation change is a major process that occurs during cancer development. Even though many tumor-related genes have been identified based on their relationship between methylation and expression, few stu-dies have been conducted to investigate the relevant biological pathways involved in these changes. To identify essential pathways likely to be affected by methylation in breast cancer, we examined a pool of genes in which expression was upregulated after induction of demethy-lation by 5-Aza-2?-deoxycytidine (Aza) in the MCF-7 breast cancer cell line. Genome-wide demethylation was confir-med by monitoring the demethylation of a previously known gene, SULT1A1. Overall, 210 and 213 genes were found to be upregulated and downregulated (fold change > 2), respectively, in common in cells treated with 5 and 10 ?M of Aza. Network analysis of these 423 genes with altered expres-sion patterns identified the involvement of a cancer related network of genes that were heavily regulated by TNF-? in breast tumorigenesis. Our results suggest that epigenetic dysregulation of cellular processes relevant to TNF-?-dependent apoptosis may be intimately involved in tumorigenesis in MCF-7 cells.

Keywords 5-Aza-2'deoxycytidine, breast cancer, genome-wide expression, MCF-7, TNF-alpha

Article

Research Article

Mol. Cells 2012; 33(2): 127-133

Published online February 29, 2012 https://doi.org/10.1007/s10059-012-2182-8

Copyright © The Korean Society for Molecular and Cellular Biology.

Expression Profiling after Induction of Demethylation in MCF-7 Breast Cancer Cells Identifies Involvement of TNF-α Mediated Cancer Pathways

Ju Hee Kim1,3, Seongeun Kang1,3, Tae Woo Kim1, Lihong Yin2, Ran Liu2,*, and Sun Jung Kim1,*

1Department of Life Science, Dongguk University-Seoul, Seoul 100-715, Korea, 2Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China, 3These authors contributed equally to this work.

Correspondence to:*Correspondence: sunjungk@dongguk.edu (SJK); ranliu@seu.edu.cn (RL)

Received: September 2, 2011; Revised: November 18, 2011; Accepted: November 18, 2011

Abstract

Epigenetic methylation change is a major process that occurs during cancer development. Even though many tumor-related genes have been identified based on their relationship between methylation and expression, few stu-dies have been conducted to investigate the relevant biological pathways involved in these changes. To identify essential pathways likely to be affected by methylation in breast cancer, we examined a pool of genes in which expression was upregulated after induction of demethy-lation by 5-Aza-2?-deoxycytidine (Aza) in the MCF-7 breast cancer cell line. Genome-wide demethylation was confir-med by monitoring the demethylation of a previously known gene, SULT1A1. Overall, 210 and 213 genes were found to be upregulated and downregulated (fold change > 2), respectively, in common in cells treated with 5 and 10 ?M of Aza. Network analysis of these 423 genes with altered expres-sion patterns identified the involvement of a cancer related network of genes that were heavily regulated by TNF-? in breast tumorigenesis. Our results suggest that epigenetic dysregulation of cellular processes relevant to TNF-?-dependent apoptosis may be intimately involved in tumorigenesis in MCF-7 cells.

Keywords: 5-Aza-2'deoxycytidine, breast cancer, genome-wide expression, MCF-7, TNF-alpha

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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