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Mol. Cells 2012; 33(2): 117-126

Published online February 29, 2012

https://doi.org/10.1007/s10059-012-2165-9

© The Korean Society for Molecular and Cellular Biology

Activation of Rice Yellow Stripe1-Like 16 (OsYSL16) Enhances Iron Efficiency

Sichul Lee1, Nayeon Ryoo2, Jong-Seong Jeon2, Mary Lou Guerinot1, and Gynheung An3,*

1Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, USA, 2Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 3Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea

Correspondence to : *Correspondence: genean@khu.ac.kr

Received: August 12, 2011; Revised: November 16, 2011; Accepted: November 17, 2011

Abstract

Graminaceous plants release ferric-chelating phytoside-rophores that bind to iron. These ferric-phytosiderophore complexes are transported across the plasma membrane by a protein produced from Yellow Stripe 1 (YS1). Here, we report the characterization of OsYSL16, one of the YS1-like genes in rice. Real-time analysis revealed that this gene was constitutively expressed irrespective of metal status. Promoter fusions of OsYSL16 to ?-glucuronidase (GUS) showed that OsYSL16 was highly expressed in the vascular tissues of the root, leaf, and spikelet, and in leaf mesophyll cells. The OsYSL16-green fluorescence protein (GFP) fusion protein was localized to the plasma membrane. From a pool of rice T-DNA insertional lines, we identified two independent activation-tagging mutants in OsYSL16. On an Fe-deficient medium, those mutants retained rela-tively high chlorophyll concentrations compared with the wild-type (WT) controls, indicating that they are more tolerant to a lack of iron. The Fe concentration in shoots was also higher in the OsYSL16 activation lines than in the WT. During germination, the rate of Fe-utilization from the seeds was higher in the OsYSL16 activation lines than in the WT seeds. Our results suggest that the function of OsYSL16 in Fe-homeostasis is to enable distribution of iron within a plant.

Keywords activation tagging, homeostasis, iron

Article

Research Article

Mol. Cells 2012; 33(2): 117-126

Published online February 29, 2012 https://doi.org/10.1007/s10059-012-2165-9

Copyright © The Korean Society for Molecular and Cellular Biology.

Activation of Rice Yellow Stripe1-Like 16 (OsYSL16) Enhances Iron Efficiency

Sichul Lee1, Nayeon Ryoo2, Jong-Seong Jeon2, Mary Lou Guerinot1, and Gynheung An3,*

1Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, USA, 2Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea, 3Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea

Correspondence to:*Correspondence: genean@khu.ac.kr

Received: August 12, 2011; Revised: November 16, 2011; Accepted: November 17, 2011

Abstract

Graminaceous plants release ferric-chelating phytoside-rophores that bind to iron. These ferric-phytosiderophore complexes are transported across the plasma membrane by a protein produced from Yellow Stripe 1 (YS1). Here, we report the characterization of OsYSL16, one of the YS1-like genes in rice. Real-time analysis revealed that this gene was constitutively expressed irrespective of metal status. Promoter fusions of OsYSL16 to ?-glucuronidase (GUS) showed that OsYSL16 was highly expressed in the vascular tissues of the root, leaf, and spikelet, and in leaf mesophyll cells. The OsYSL16-green fluorescence protein (GFP) fusion protein was localized to the plasma membrane. From a pool of rice T-DNA insertional lines, we identified two independent activation-tagging mutants in OsYSL16. On an Fe-deficient medium, those mutants retained rela-tively high chlorophyll concentrations compared with the wild-type (WT) controls, indicating that they are more tolerant to a lack of iron. The Fe concentration in shoots was also higher in the OsYSL16 activation lines than in the WT. During germination, the rate of Fe-utilization from the seeds was higher in the OsYSL16 activation lines than in the WT seeds. Our results suggest that the function of OsYSL16 in Fe-homeostasis is to enable distribution of iron within a plant.

Keywords: activation tagging, homeostasis, iron

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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