Su Man Lee" /> Su Man Lee, Yeon Kyung Na, Hae Sook Hong, Eun Jeong Jang, Ghil Suk Yoon, Jae Yong Park*, and Dong Sun Kim*

" /> Su Man Lee, Yeon Kyung Na, Hae Sook Hong, Eun Jeong Jang, Ghil Suk Yoon, Jae Yong Park*, and Dong Sun Kim*

. Mol. Cells 2011;32:343-8. https://doi.org/10.1007/s10059-011-0073-z">
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Mol. Cells 2011; 32(4): 343-348

Published online October 31, 2011

https://doi.org/10.1007/s10059-011-0073-z

© The Korean Society for Molecular and Cellular Biology

Hypomethylation of the Thymosin β10 Gene Is Not Associated with Its Overexpression in Non-Small Cell Lung Cancer

Su Man Lee1, Yeon Kyung Na2, Hae Sook Hong2, Eun Jeong Jang3, Ghil Suk Yoon3, Jae Yong Park4,*, and Dong Sun Kim1,*

1Department of Anatomy, School of Medicine, Kyungpook National University, Daegu 702-422, Korea, 2College of Nursing, Kyungpook National University, Daegu 702-422, Korea, 3Department of Pathology, School of Medicine, Kyungpook National University, Daegu 702-422, Korea, 4Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 702-422, Korea

Correspondence to : *Correspondence: doskim@knu.ac.kr (DSK); jaeyong@knu.ac.kr (JYP)

Received: April 14, 2011; Revised: July 30, 2011; Accepted: August 10, 2011

Abstract

Lung cancer is the leading cause of cancer-related deaths worldwide and is usually associated with a late diagnosis and a poor prognosis. Thymosin beta10 (TMSB10) is a mono-meric actin sequestering protein that regulates actin cytoskeleton organization. The aberrant TMSB10 expression has been implicated in the pathogenesis of human cancers. However, its role in carcinogenesis is still controversial. To better understand the role of TMSB10 in lung tumorigenesis and its regulatory mechanism, we examined the methylation status and expression of the TMSB10 gene in non-small cell lung cancers (NSCLCs) using methylation-specific PCR (MSP) and immunohistochemistry (IHC), respectively. MSP analysis showed that the TMSB10 promoter was already unmethylated in most tumor tissues and became demethylated in 20 (14.4%) of the 139 NSCLCs. TMSB10 hypomethylation was not significantly correlated with the clinicopathological features. IHC showed that the TMSB10 protein was strongly expressed in the cytoplasm of malignant cells and its overexpression was detected in 50.0% of the tumor tissues compared to normal tissues. TMSB10 overexpression was frequently observed in sqau-mous cell carcinomas compared to adenocarcinomas with border line significance (P = 0.072). However, TMSB10 methylation status was not linked to its overexpression. Collectively, these results suggest that TMSB10 hypome-thylation may be a frequent event in NSCLCs, but it may not be a common mechanism underlying TMSB10 overexpression. However, further studies with large num-bers of patients are needed to confirm our findings.

Keywords hypomethylation, immunohistochemistry, methylation-specific PCR, non-small cell lung cancer, thymosin beta10

Article

Research Article

Mol. Cells 2011; 32(4): 343-348

Published online October 31, 2011 https://doi.org/10.1007/s10059-011-0073-z

Copyright © The Korean Society for Molecular and Cellular Biology.

Hypomethylation of the Thymosin β10 Gene Is Not Associated with Its Overexpression in Non-Small Cell Lung Cancer

Su Man Lee1, Yeon Kyung Na2, Hae Sook Hong2, Eun Jeong Jang3, Ghil Suk Yoon3, Jae Yong Park4,*, and Dong Sun Kim1,*

1Department of Anatomy, School of Medicine, Kyungpook National University, Daegu 702-422, Korea, 2College of Nursing, Kyungpook National University, Daegu 702-422, Korea, 3Department of Pathology, School of Medicine, Kyungpook National University, Daegu 702-422, Korea, 4Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 702-422, Korea

Correspondence to:*Correspondence: doskim@knu.ac.kr (DSK); jaeyong@knu.ac.kr (JYP)

Received: April 14, 2011; Revised: July 30, 2011; Accepted: August 10, 2011

Abstract

Lung cancer is the leading cause of cancer-related deaths worldwide and is usually associated with a late diagnosis and a poor prognosis. Thymosin beta10 (TMSB10) is a mono-meric actin sequestering protein that regulates actin cytoskeleton organization. The aberrant TMSB10 expression has been implicated in the pathogenesis of human cancers. However, its role in carcinogenesis is still controversial. To better understand the role of TMSB10 in lung tumorigenesis and its regulatory mechanism, we examined the methylation status and expression of the TMSB10 gene in non-small cell lung cancers (NSCLCs) using methylation-specific PCR (MSP) and immunohistochemistry (IHC), respectively. MSP analysis showed that the TMSB10 promoter was already unmethylated in most tumor tissues and became demethylated in 20 (14.4%) of the 139 NSCLCs. TMSB10 hypomethylation was not significantly correlated with the clinicopathological features. IHC showed that the TMSB10 protein was strongly expressed in the cytoplasm of malignant cells and its overexpression was detected in 50.0% of the tumor tissues compared to normal tissues. TMSB10 overexpression was frequently observed in sqau-mous cell carcinomas compared to adenocarcinomas with border line significance (P = 0.072). However, TMSB10 methylation status was not linked to its overexpression. Collectively, these results suggest that TMSB10 hypome-thylation may be a frequent event in NSCLCs, but it may not be a common mechanism underlying TMSB10 overexpression. However, further studies with large num-bers of patients are needed to confirm our findings.

Keywords: hypomethylation, immunohistochemistry, methylation-specific PCR, non-small cell lung cancer, thymosin beta10

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
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Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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