Jong Min Choi" /> Jong Min Choi, So Jung Chu, Kyong Hoon Ahn, Seok Kyun Kim, Jung Eun Ji, Jong Hoon Won, Hyung Chul Kim, Moon Jung Back, and Dae Kyong Kim*

" /> Jong Min Choi, So Jung Chu, Kyong Hoon Ahn, Seok Kyun Kim, Jung Eun Ji, Jong Hoon Won, Hyung Chul Kim, Moon Jung Back, and Dae Kyong Kim*

. Mol. Cells 2011;32:325-31. https://doi.org/10.1007/s10059-011-1034-2">
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Mol. Cells 2011; 32(4): 325-331

Published online October 31, 2011

https://doi.org/10.1007/s10059-011-1034-2

© The Korean Society for Molecular and Cellular Biology

C6-Ceramide Enhances Phagocytic Activity of Kupffer Cells through the Production of Endogenous Ceramides

Jong Min Choi1,2, So Jung Chu1,2, Kyong Hoon Ahn1, Seok Kyun Kim1, Jung Eun Ji1, Jong Hoon Won1, Hyung Chul Kim1, Moon Jung Back1, and Dae Kyong Kim1,*

1Department of Environmental and Health Chemistry, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea, 2These authors contributed equally to this work.

Correspondence to : *Correspondence: proteinlab@hanmail.net

Received: February 25, 2011; Revised: June 15, 2011; Accepted: July 11, 2011

Abstract

Ceramide has been suggested to be not only a tumor-suppressive lipid but also a regulator of phagocytosis. We examined whether exogenous cell-permeable C6-ceramide enhances the phagocytic activity of Kupffer cells (KCs) and affects the level of cellular ceramides. Rat KCs were isolated by collagenase digestion and differential centrifugation, using Percoll system. Phagocytic activity was measured by FACS analysis after incubating KCs with fluorescence-conjugated latex beads, and the level of cellular ceramide was analyzed by liquid chromatography tandem-mass spectrometry (LC-MS/MS). In this study we found that permeable C6-ceramide increases the cellular levels of endogenous ceramides via a sphingosine-re-cycling pathway leading to enhanced phagocytosis by KCs.

Keywords ceramide, kupffer cells, phagocytosis, sphingomyelin, sphingosine-recycle

Article

Research Article

Mol. Cells 2011; 32(4): 325-331

Published online October 31, 2011 https://doi.org/10.1007/s10059-011-1034-2

Copyright © The Korean Society for Molecular and Cellular Biology.

C6-Ceramide Enhances Phagocytic Activity of Kupffer Cells through the Production of Endogenous Ceramides

Jong Min Choi1,2, So Jung Chu1,2, Kyong Hoon Ahn1, Seok Kyun Kim1, Jung Eun Ji1, Jong Hoon Won1, Hyung Chul Kim1, Moon Jung Back1, and Dae Kyong Kim1,*

1Department of Environmental and Health Chemistry, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea, 2These authors contributed equally to this work.

Correspondence to:*Correspondence: proteinlab@hanmail.net

Received: February 25, 2011; Revised: June 15, 2011; Accepted: July 11, 2011

Abstract

Ceramide has been suggested to be not only a tumor-suppressive lipid but also a regulator of phagocytosis. We examined whether exogenous cell-permeable C6-ceramide enhances the phagocytic activity of Kupffer cells (KCs) and affects the level of cellular ceramides. Rat KCs were isolated by collagenase digestion and differential centrifugation, using Percoll system. Phagocytic activity was measured by FACS analysis after incubating KCs with fluorescence-conjugated latex beads, and the level of cellular ceramide was analyzed by liquid chromatography tandem-mass spectrometry (LC-MS/MS). In this study we found that permeable C6-ceramide increases the cellular levels of endogenous ceramides via a sphingosine-re-cycling pathway leading to enhanced phagocytosis by KCs.

Keywords: ceramide, kupffer cells, phagocytosis, sphingomyelin, sphingosine-recycle

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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