Handong Yang" /> *

" /> Handong Yang, Yinfang Wang, Hang Qian, Peng Zhang*, and Congxin Huang*

" /> Handong Yang, Yinfang Wang, Hang Qian, Peng Zhang*, and Congxin Huang*

. Mol. Cells 2011;32:235-41. https://doi.org/10.1007/s10059-011-1026-2">
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Mol. Cells 2011; 32(3): 235-241

Published online September 30, 2011

https://doi.org/10.1007/s10059-011-1026-2

© The Korean Society for Molecular and Cellular Biology

Pim Protein Kinase-3 Is Regulated by TNF-α and Promotes Endothelial Cell Sprouting

Handong Yang1, Yinfang Wang2, Hang Qian2, Peng Zhang2,*, and Congxin Huang1,*

1Department of Cardiovascular Diseases, Renmin Hospital of Wuhan University, Wuhan City, China, 2Institute of Cardiovascular Science, Dongfeng Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China

Correspondence to : *Correspondence: huangcongxinhb@yahoo.cn (CH); zhangnep@yahoo.cn (PZ)

Received: February 14, 2011; Revised: June 28, 2011; Accepted: June 29, 2011

Abstract

Tumor necrosis factor-α (TNF-α) plays an important role in pathological angiogenesis associated with inflammatory response. Pim-3 kinase belonging to serine/threonine protein kinases is a potent suppressor of myc-induced apoptosis. We have recently demonstrated that Pim-3 plays an essential role in endothelial cell (EC) spreading and migration. In this study, we showed that TNF-α transiently increased Pim-3 mRNA expression, and this was mediated through Tumor necrosis factor-α receptor-1 (TNFR1) pathway in ECs. TNF-α could promote stabilization of Pim-3 mRNA in ECs. Small-interfering RNA (siRNA)-mediated gene knockdown of Pim-3 significantly impaired TNF-α-induced formation of EC membrane protrusions in vitro. Furthermore, Pim-3 silencing inhibited EC sprouting in subcutaneous Matrigel in vivo. eNOS mRNA abundance was lower in Pim-3 siRNA transfected ECs compared with the control ECs. These observations suggest that Pim-3 plays a role in TNF-α-induced angiogenesis.

Keywords angiogenesis, endothelial cell, Pim-3, TNF-α

Article

Research Article

Mol. Cells 2011; 32(3): 235-241

Published online September 30, 2011 https://doi.org/10.1007/s10059-011-1026-2

Copyright © The Korean Society for Molecular and Cellular Biology.

Pim Protein Kinase-3 Is Regulated by TNF-α and Promotes Endothelial Cell Sprouting

Handong Yang1, Yinfang Wang2, Hang Qian2, Peng Zhang2,*, and Congxin Huang1,*

1Department of Cardiovascular Diseases, Renmin Hospital of Wuhan University, Wuhan City, China, 2Institute of Cardiovascular Science, Dongfeng Hospital, Hubei University of Medicine, Shiyan City, Hubei Province, China

Correspondence to:*Correspondence: huangcongxinhb@yahoo.cn (CH); zhangnep@yahoo.cn (PZ)

Received: February 14, 2011; Revised: June 28, 2011; Accepted: June 29, 2011

Abstract

Tumor necrosis factor-α (TNF-α) plays an important role in pathological angiogenesis associated with inflammatory response. Pim-3 kinase belonging to serine/threonine protein kinases is a potent suppressor of myc-induced apoptosis. We have recently demonstrated that Pim-3 plays an essential role in endothelial cell (EC) spreading and migration. In this study, we showed that TNF-α transiently increased Pim-3 mRNA expression, and this was mediated through Tumor necrosis factor-α receptor-1 (TNFR1) pathway in ECs. TNF-α could promote stabilization of Pim-3 mRNA in ECs. Small-interfering RNA (siRNA)-mediated gene knockdown of Pim-3 significantly impaired TNF-α-induced formation of EC membrane protrusions in vitro. Furthermore, Pim-3 silencing inhibited EC sprouting in subcutaneous Matrigel in vivo. eNOS mRNA abundance was lower in Pim-3 siRNA transfected ECs compared with the control ECs. These observations suggest that Pim-3 plays a role in TNF-α-induced angiogenesis.

Keywords: angiogenesis, endothelial cell, Pim-3, TNF-α

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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