Wanhui Kim" /> Wanhui Kim, Hae Ji Ahn, Tzyy-Jen Chiou, and Ji Hoon Ahn*" /> Wanhui Kim, Hae Ji Ahn, Tzyy-Jen Chiou, and Ji Hoon Ahn*. Mol. Cells 2011;32:83-8. https://doi.org/10.1007/s10059-011-1043-1">
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Mol. Cells 2011; 32(1): 83-88

Published online July 31, 2011

https://doi.org/10.1007/s10059-011-1043-1

© The Korean Society for Molecular and Cellular Biology

The Role of the miR399-PHO2 Module in the Regulation of Flowering Time in Response to Different Ambient Temperatures in Arabidopsis thaliana

Wanhui Kim, Hae Ji Ahn, Tzyy-Jen Chiou1, and Ji Hoon Ahn*

Creative Research Initiatives, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea, 1Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taiwan, ROC

Correspondence to : *Correspondence: jahn@korea.ac.kr

Received: March 8, 2011; Revised: March 28, 2011; Accepted: March 31, 2011

Abstract

A moderate change in ambient temperature significantly affects plant physiology including flowering time. MiR399 and its target gene PHOSPHATE 2 (PHO2) are known to play a role in the maintenance of phosphate homeostasis. However, the regulation of flowering time by the miR399-PHO2 module has not been investigated. As we have previously identified miR399 as an ambient temperature-res-ponsive miRNA, we further investigated whether a change in expression of the miR399-PHO2 module affects flowering time in response to ambient temperature changes. Here, we showed that miR399b-overexpressing plants and a loss-of-function allele of PHO2 (pho2) exhibited an early flowering phenotype only at normal temperature (23oC). Interestingly, their flowering time at lower temperature (16ºC) was similar to that of wild-type plants, suggesting that alteration in flowering time by miR399 and its target PHO2 was seen only at normal temperature (23oC). Flowering time ratio (16oC/23oC) revealed that miR399b-overex-pressing plants and pho2 mutants showed increased sensitivity to ambient temperature changes. Expression analysis indicated that expression of TWIN SISTER OF FT (TSF) was increased in miR399b-overexpressing plants and pho2 mutants at 23oC, suggesting that their early flo-wering phenotype is associated with TSF upregulation. Taken together, our results suggest that miR399, an am-bient temperature-responsive miRNA, plays a role in am-bient temperature-responsive flowering in Arabidopsis.

Keywords ambient temperature, flowering time, MiR399, PHO2, TSF

Article

Research Article

Mol. Cells 2011; 32(1): 83-88

Published online July 31, 2011 https://doi.org/10.1007/s10059-011-1043-1

Copyright © The Korean Society for Molecular and Cellular Biology.

The Role of the miR399-PHO2 Module in the Regulation of Flowering Time in Response to Different Ambient Temperatures in Arabidopsis thaliana

Wanhui Kim, Hae Ji Ahn, Tzyy-Jen Chiou1, and Ji Hoon Ahn*

Creative Research Initiatives, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea, 1Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taiwan, ROC

Correspondence to:*Correspondence: jahn@korea.ac.kr

Received: March 8, 2011; Revised: March 28, 2011; Accepted: March 31, 2011

Abstract

A moderate change in ambient temperature significantly affects plant physiology including flowering time. MiR399 and its target gene PHOSPHATE 2 (PHO2) are known to play a role in the maintenance of phosphate homeostasis. However, the regulation of flowering time by the miR399-PHO2 module has not been investigated. As we have previously identified miR399 as an ambient temperature-res-ponsive miRNA, we further investigated whether a change in expression of the miR399-PHO2 module affects flowering time in response to ambient temperature changes. Here, we showed that miR399b-overexpressing plants and a loss-of-function allele of PHO2 (pho2) exhibited an early flowering phenotype only at normal temperature (23oC). Interestingly, their flowering time at lower temperature (16ºC) was similar to that of wild-type plants, suggesting that alteration in flowering time by miR399 and its target PHO2 was seen only at normal temperature (23oC). Flowering time ratio (16oC/23oC) revealed that miR399b-overex-pressing plants and pho2 mutants showed increased sensitivity to ambient temperature changes. Expression analysis indicated that expression of TWIN SISTER OF FT (TSF) was increased in miR399b-overexpressing plants and pho2 mutants at 23oC, suggesting that their early flo-wering phenotype is associated with TSF upregulation. Taken together, our results suggest that miR399, an am-bient temperature-responsive miRNA, plays a role in am-bient temperature-responsive flowering in Arabidopsis.

Keywords: ambient temperature, flowering time, MiR399, PHO2, TSF

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
COVER PICTURE
Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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