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Mol. Cells 2011; 31(6): 553-561

Published online June 30, 2011

https://doi.org/10.1007/s10059-011-1038-y

© The Korean Society for Molecular and Cellular Biology

Identification and Characterization of the Du-plicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Jung-Il Cho, Hyun-Bi Kim, Chi-Yeol Kim, Tae-Ryong Hahn, and Jong-Seong Jeon*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea

Correspondence to : *Correspondence: jjeon@khu.ac.kr

Received: March 4, 2011; Revised: March 21, 2011; Accepted: March 22, 2011

Abstract

Systematic searches using the complete genome se-quence of rice (Oryza sativa) identified OsSUS7, a new member of the rice sucrose synthase (OsSUS) gene family, which shows only nine single nucleotide substitutions in the OsSUS5 coding sequence. Comparative genomic analysis revealed that the synteny between OsSUS5 and OsSUS7 is conserved, and that significant numbers of transposable elements are scattered at both loci. In particular, a 17.6-kb genomic region containing transposable elements was identified in the 5' upstream sequence of the OsSUS7 gene. GFP fusion experiments indicated that OsSUS5 and OsSUS7 are largely associated with the plasma membrane and partly with the cytosol in maize mesophyll protoplasts. RT-PCR analysis and transient expression assays revealed that OsSUS5 and OsSUS7 exhibit similar expression patterns in rice tissues, with the highest ex-pression evident in roots. These results suggest that two redundant genes, OsSUS5 and OsSUS7, evolved via dup-lication of a chromosome region and through the transposition of transposable elements.

Keywords duplication, localization, rice, sucrose synthase, transposable element

Article

Research Article

Mol. Cells 2011; 31(6): 553-561

Published online June 30, 2011 https://doi.org/10.1007/s10059-011-1038-y

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification and Characterization of the Du-plicate Rice Sucrose Synthase Genes OsSUS5 and OsSUS7 Which Are Associated with the Plasma Membrane

Jung-Il Cho, Hyun-Bi Kim, Chi-Yeol Kim, Tae-Ryong Hahn, and Jong-Seong Jeon*

Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea

Correspondence to:*Correspondence: jjeon@khu.ac.kr

Received: March 4, 2011; Revised: March 21, 2011; Accepted: March 22, 2011

Abstract

Systematic searches using the complete genome se-quence of rice (Oryza sativa) identified OsSUS7, a new member of the rice sucrose synthase (OsSUS) gene family, which shows only nine single nucleotide substitutions in the OsSUS5 coding sequence. Comparative genomic analysis revealed that the synteny between OsSUS5 and OsSUS7 is conserved, and that significant numbers of transposable elements are scattered at both loci. In particular, a 17.6-kb genomic region containing transposable elements was identified in the 5' upstream sequence of the OsSUS7 gene. GFP fusion experiments indicated that OsSUS5 and OsSUS7 are largely associated with the plasma membrane and partly with the cytosol in maize mesophyll protoplasts. RT-PCR analysis and transient expression assays revealed that OsSUS5 and OsSUS7 exhibit similar expression patterns in rice tissues, with the highest ex-pression evident in roots. These results suggest that two redundant genes, OsSUS5 and OsSUS7, evolved via dup-lication of a chromosome region and through the transposition of transposable elements.

Keywords: duplication, localization, rice, sucrose synthase, transposable element

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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