Sang-Je Park" /> Sang-Je Park, Jae-Won Huh, Young-Hyun Kim, Ji-Su Kim, Bong-Seok Song, Sang-Rae Lee, Sun-Uk Kim, Heui-Soo Kim, Kazuhiko Imakawa, and Kyu-Tae Chang*" /> Sang-Je Park, Jae-Won Huh, Young-Hyun Kim, Ji-Su Kim, Bong-Seok Song, Sang-Rae Lee, Sun-Uk Kim, Heui-Soo Kim, Kazuhiko Imakawa, and Kyu-Tae Chang*. Mol. Cells 2011;31:431-6. https://doi.org/10.1007/s10059-011-0272-7">
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Mol. Cells 2011; 31(5): 431-436

Published online February 22, 2011

https://doi.org/10.1007/s10059-011-0272-7

© The Korean Society for Molecular and Cellular Biology

Quantitative Analysis of Retromer Complex-Related Genes during Embryo Development in the Mouse

Sang-Je Park1,2,5, Jae-Won Huh1,5, Young-Hyun Kim1,3,5, Ji-Su Kim1,5, Bong-Seok Song1, Sang-Rae Lee1, Sun-Uk Kim1, Heui-Soo Kim2, Kazuhiko Imakawa4, and Kyu-Tae Chang1,3,*

1National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 363-883, Korea, 2Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Korea, 3Department of Functional Genomics, University of Science and Technology, Daejeon 305-333, Korea, 4Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan, 5These authors contributed equally to this work.

Correspondence to : *Correspondence: changkt@kribb.re.kr

Received: November 2, 2011; Revised: January 27, 2011; Accepted: February 9, 2011

Abstract

The retromer complex is a heteropentameric protein unit associated with retrograde transport of cargo proteins from endosomes to the trans-Golgi network. Functional silencing study of the Vps26a gene indicated the important role of the retromer complex during early developmental stages in the mouse. However, individual expression patterns and quantitative analysis of individual members of the retromer complex during the early developmental stages has not been investigated. In this study, we conducted quantitative expression analysis of six retromer complex genes (Vps26a, Vps26b, Vps29, Vps35, Snx1, and Snx2) and one related receptor gene (Ci-mpr) during the eleven embryonic stages with normal MEF (mouse embryonic fibroblast) and Vps26a-/- MEF cells. Remarkably, except for Vps26a (maternal expression pattern), all tested genes showed maternal-zygotic expression patterns. And five genes (Vps26b, Vps29, Vps35, Snx2, and Ci-mpr) showed a pattern of decreased expression in Vps26a-/- MEF cells by comparative analysis between normal MEF and Vps26a-/- MEF cells. However, the Snx1 gene showed a pattern of increased expression in Vps26a-/- MEF cells. From our results, we could assume that retromer complex-related genes have important roles during oocyte development. However, in the preimplantation stage, they did not have significant roles.

Keywords mouse embryo, mouse embryonic fibroblast, real-time RT-PCR, retromer complex

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Research Article

Mol. Cells 2011; 31(5): 431-436

Published online May 31, 2011 https://doi.org/10.1007/s10059-011-0272-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Quantitative Analysis of Retromer Complex-Related Genes during Embryo Development in the Mouse

Sang-Je Park1,2,5, Jae-Won Huh1,5, Young-Hyun Kim1,3,5, Ji-Su Kim1,5, Bong-Seok Song1, Sang-Rae Lee1, Sun-Uk Kim1, Heui-Soo Kim2, Kazuhiko Imakawa4, and Kyu-Tae Chang1,3,*

1National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 363-883, Korea, 2Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Korea, 3Department of Functional Genomics, University of Science and Technology, Daejeon 305-333, Korea, 4Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan, 5These authors contributed equally to this work.

Correspondence to:*Correspondence: changkt@kribb.re.kr

Received: November 2, 2011; Revised: January 27, 2011; Accepted: February 9, 2011

Abstract

The retromer complex is a heteropentameric protein unit associated with retrograde transport of cargo proteins from endosomes to the trans-Golgi network. Functional silencing study of the Vps26a gene indicated the important role of the retromer complex during early developmental stages in the mouse. However, individual expression patterns and quantitative analysis of individual members of the retromer complex during the early developmental stages has not been investigated. In this study, we conducted quantitative expression analysis of six retromer complex genes (Vps26a, Vps26b, Vps29, Vps35, Snx1, and Snx2) and one related receptor gene (Ci-mpr) during the eleven embryonic stages with normal MEF (mouse embryonic fibroblast) and Vps26a-/- MEF cells. Remarkably, except for Vps26a (maternal expression pattern), all tested genes showed maternal-zygotic expression patterns. And five genes (Vps26b, Vps29, Vps35, Snx2, and Ci-mpr) showed a pattern of decreased expression in Vps26a-/- MEF cells by comparative analysis between normal MEF and Vps26a-/- MEF cells. However, the Snx1 gene showed a pattern of increased expression in Vps26a-/- MEF cells. From our results, we could assume that retromer complex-related genes have important roles during oocyte development. However, in the preimplantation stage, they did not have significant roles.

Keywords: mouse embryo, mouse embryonic fibroblast, real-time RT-PCR, retromer complex

Mol. Cells
May 31, 2023 Vol.46 No.5, pp. 259~328
COVER PICTURE
The alpha-helices in the lamin filaments are depicted as coils, with different subdomains distinguished by various colors. Coil 1a is represented by magenta, coil 1b by yellow, L2 by green, coil 2a by white, coil 2b by brown, stutter by cyan, coil 2c by dark blue, and the lamin Ig-like domain by grey. In the background, cells are displayed, with the cytosol depicted in green and the nucleus in blue (Ahn et al., pp. 309-318).

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