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Mol. Cells 2011; 31(3): 247-253

Published online March 31, 2011

https://doi.org/10.1007/s10059-011-0033-7

© The Korean Society for Molecular and Cellular Biology

Identification and Characterization of RNF2 Response Elements in Human Kidney Cells

Dongwon Choi, and Seongman Kang*

Graduate School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea

Correspondence to : *Correspondence: skang@korea.ac.kr

Received: September 10, 2010; Revised: December 1, 2010; Accepted: December 17, 2010

Abstract

RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.

Keywords PcG complex, RNF2, RNF2-responding loci

Article

Research Article

Mol. Cells 2011; 31(3): 247-253

Published online March 31, 2011 https://doi.org/10.1007/s10059-011-0033-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification and Characterization of RNF2 Response Elements in Human Kidney Cells

Dongwon Choi, and Seongman Kang*

Graduate School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea

Correspondence to:*Correspondence: skang@korea.ac.kr

Received: September 10, 2010; Revised: December 1, 2010; Accepted: December 17, 2010

Abstract

RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.

Keywords: PcG complex, RNF2, RNF2-responding loci

Mol. Cells
Dec 31, 2021 Vol.44 No.12, pp. 861~919
COVER PICTURE
Structure of the fly peripheral neurons in the fly head. Flies have basic sensory organs including eyes for vision, antennae and maxillary palps for olfaction, and proboscis (magenta) for gustation which can be labelled with monoclonal antibody 22C10. The figure is a 3D reconstructed image with 30 slices of confocal sections with 3 μm interval. It shows that the proboscis is required for sensing attractive carboxylic acids such as glycolic acid, citric acid, and lactic acid (Shrestha and Lee, pp. 900-910).

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