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Mol. Cells 2011; 31(1): 33-38

Published online January 31, 2011

https://doi.org/10.1007/s10059-011-0009-7

© The Korean Society for Molecular and Cellular Biology

Murine Hepatic miRNAs Expression and Regula-tion of Gene Expression in Diet-Induced Obese Mice

Jae-Ho Park, Jiyun Ahn, Suna Kim, Dae Young Kwon, and Tae Youl Ha*

Korea Food Research Institute, Sungnam 463-746, Korea

Correspondence to : *Correspondence: tyhap@kfri.re.kr

Received: June 15, 2010; Accepted: October 20, 2010

Abstract

MicroRNAs are short, non-coding RNA molecules that regulate gene expression primarily by translational re-pression or by messenger RNA degradation. MicroRNAs play crucial roles in various biological processes. However, little is known regarding their role in obesity. We investigated differences of microRNA (miRNA) expression in liver tissue from diet-induced obese mice and potential effects of them on gene and protein expression. We used a miRNA microarray and quantitative RT-PCR to determine miRNA expression in murine liver tissue. Gene and protein expression were determined by qRT-PCR and Western blot analysis. Effects of miRNA by knock-down using RNAi or overexpression on putative target genes and/or proteins in a murine hepatic cell line were also investigated. MicroRNA array and qRT-PCR analsysis revealed that > 50 miRNAs were down- or upregulated more than 2-fold in the liver of diet-induced obese mice. While changes in expression of many genes were observed at the mRNA level, some were only altered at the protein level. Overexpression or knock-down of miR-107 in murine hepatic cells revealed that the expression of its putative target, fatty acid synthase, was dramatically decreased or increased, respectively. In conclusion, more than 50 he-patic miRNAs were dysregulated in diet-induced obese mice. Some of them regulate protein expression at translation level and others regulate mRNA expression at transcriptional level. MiR-107 is downregulated while FASN, a putative target of miR-107, was increased in diet-induced obese mice. These findings provide the evidence of the correlation of miRNAs and their targets in diet-induced obese mice.

Keywords gene expression, high-fat diet, microRNA, miRNA array, obesity

Article

Research Article

Mol. Cells 2011; 31(1): 33-38

Published online January 31, 2011 https://doi.org/10.1007/s10059-011-0009-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Murine Hepatic miRNAs Expression and Regula-tion of Gene Expression in Diet-Induced Obese Mice

Jae-Ho Park, Jiyun Ahn, Suna Kim, Dae Young Kwon, and Tae Youl Ha*

Korea Food Research Institute, Sungnam 463-746, Korea

Correspondence to:*Correspondence: tyhap@kfri.re.kr

Received: June 15, 2010; Accepted: October 20, 2010

Abstract

MicroRNAs are short, non-coding RNA molecules that regulate gene expression primarily by translational re-pression or by messenger RNA degradation. MicroRNAs play crucial roles in various biological processes. However, little is known regarding their role in obesity. We investigated differences of microRNA (miRNA) expression in liver tissue from diet-induced obese mice and potential effects of them on gene and protein expression. We used a miRNA microarray and quantitative RT-PCR to determine miRNA expression in murine liver tissue. Gene and protein expression were determined by qRT-PCR and Western blot analysis. Effects of miRNA by knock-down using RNAi or overexpression on putative target genes and/or proteins in a murine hepatic cell line were also investigated. MicroRNA array and qRT-PCR analsysis revealed that > 50 miRNAs were down- or upregulated more than 2-fold in the liver of diet-induced obese mice. While changes in expression of many genes were observed at the mRNA level, some were only altered at the protein level. Overexpression or knock-down of miR-107 in murine hepatic cells revealed that the expression of its putative target, fatty acid synthase, was dramatically decreased or increased, respectively. In conclusion, more than 50 he-patic miRNAs were dysregulated in diet-induced obese mice. Some of them regulate protein expression at translation level and others regulate mRNA expression at transcriptional level. MiR-107 is downregulated while FASN, a putative target of miR-107, was increased in diet-induced obese mice. These findings provide the evidence of the correlation of miRNAs and their targets in diet-induced obese mice.

Keywords: gene expression, high-fat diet, microRNA, miRNA array, obesity

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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