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Mol. Cells 2010; 29(5): 501-507

Published online May 31, 2010

https://doi.org/10.1007/s10059-010-0062-7

© The Korean Society for Molecular and Cellular Biology

Fgfbp1 Is Essential for the Cellular Survival dur-ing Zebrafish Embryogenesis

Hae-ock Lee*, Hyerim Choe, Kyungwoon Seo, Hyunsook Lee, Jinseon Lee1, and Jhingook Kim2

Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea, 1Department of Thoracic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea, 2Cancer Research Division, Center for Clinical Research, Samsung Biomedical Research Institute, Seoul 135-710, Korea

Correspondence to : *Correspondence: haeockl@snu.ac.kr

Received: November 30, 2010; Revised: January 12, 2010; Accepted: January 12, 2010

Abstract

Fibroblast growth factor binding protein 1 (FGFBP1) is expressed in various tumors and may serve as a diagnos-tic marker and/or a therapeutic target. Previous studies suggested FGFBP1 functions as an angiogenic switch molecule by regulating the activity of FGF2, and it was later found to associate with a broad spectrum of FGFs. To study FGFBP1, we used zebrafish, in which the function of extracellular matrix protein can be easily studied in intact tissues or organisms. When Fgfbp1 expression was knocked down, morphants manifested massive cell death and structural abnormalities. Cell death was most prominent in the brain and the neural tube, but not limited to those regions. These findings suggest that the primary function of Fgfbp1 may be to sustain cellular survival throughout embryogenesis. For comparison, the expression of fgf2 was limited to the early stage of embryogenesis and fgf2 morphants showed more severe phenotype, with high morbidity before reaching 14-somites. Taken together, our work reveals the physiologic function of Fgfbp1, and that its function could be exerted in a Fgf2-independent manner.

Keywords Apoptosis, embryogenesis, FGFBP1, FGF2, zebrafish

Article

Research Article

Mol. Cells 2010; 29(5): 501-507

Published online May 31, 2010 https://doi.org/10.1007/s10059-010-0062-7

Copyright © The Korean Society for Molecular and Cellular Biology.

Fgfbp1 Is Essential for the Cellular Survival dur-ing Zebrafish Embryogenesis

Hae-ock Lee*, Hyerim Choe, Kyungwoon Seo, Hyunsook Lee, Jinseon Lee1, and Jhingook Kim2

Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Korea, 1Department of Thoracic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea, 2Cancer Research Division, Center for Clinical Research, Samsung Biomedical Research Institute, Seoul 135-710, Korea

Correspondence to:*Correspondence: haeockl@snu.ac.kr

Received: November 30, 2010; Revised: January 12, 2010; Accepted: January 12, 2010

Abstract

Fibroblast growth factor binding protein 1 (FGFBP1) is expressed in various tumors and may serve as a diagnos-tic marker and/or a therapeutic target. Previous studies suggested FGFBP1 functions as an angiogenic switch molecule by regulating the activity of FGF2, and it was later found to associate with a broad spectrum of FGFs. To study FGFBP1, we used zebrafish, in which the function of extracellular matrix protein can be easily studied in intact tissues or organisms. When Fgfbp1 expression was knocked down, morphants manifested massive cell death and structural abnormalities. Cell death was most prominent in the brain and the neural tube, but not limited to those regions. These findings suggest that the primary function of Fgfbp1 may be to sustain cellular survival throughout embryogenesis. For comparison, the expression of fgf2 was limited to the early stage of embryogenesis and fgf2 morphants showed more severe phenotype, with high morbidity before reaching 14-somites. Taken together, our work reveals the physiologic function of Fgfbp1, and that its function could be exerted in a Fgf2-independent manner.

Keywords: Apoptosis, embryogenesis, FGFBP1, FGF2, zebrafish

Mol. Cells
Sep 30, 2022 Vol.45 No.9, pp. 603~672
COVER PICTURE
The Target of Rapamycin Complex (TORC) is a central regulatory hub in eukaryotes, which is well conserved in diverse plant species, including tomato (Solanum lycopersicum). Inhibition of TORC genes (SlTOR, SlLST8, and SlRAPTOR) by VIGS (virus-induced gene silencing) results in early fruit ripening in tomato. The red/ orange tomatoes are early-ripened TORC-silenced fruits, while the green tomato is a control fruit. Top, left, control fruit (TRV2-myc); top, right, TRV2-SlLST8; bottom, left, TRV2-SlTOR; bottom, right, TRV2-SlRAPTOR(Choi et al., pp. 660-672).

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