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Mol. Cells 2009; 28(6): 553-558

Published online December 31, 2009

https://doi.org/10.1007/s10059-009-0150-8

© The Korean Society for Molecular and Cellular Biology

Twist2 Regulates CD7 Expression and
Galectin-1-Induced Apoptosis in Mature T-Cells

Han Seok Koh, Changjin Lee, Kwang Soo Lee, Eun Jung Park, Rho H. Seong, Seokmann Hong, and Sung Ho Jeon

Received: August 5, 2009; Revised: September 15, 2009; Accepted: September 17, 2009

Abstract

In the periphery, a galectin-1 receptor, CD7, plays crucial roles in galectin-1-mediated apoptosis of activated T-cells as well as progression of T-lymphoma. Previously, we demonstrated that NF-κB downregulated CD7 gene expression through the p38 MAPK pathway in developing immature thymocytes. However, its regulatory pathway is not well understood in functional mature T-cells. Here, we show that CD7 expression was downregulated by Twist2 in Jurkat cells, a human acute T-cell lymphoma cell line, and in EL4 cells, a mature mur-ine T-cell lymphoma cell line. Furthermore, ectopic expression of Twist2 in Jurkat cells reduced galectin-1-induced apoptosis. While full-length Twist2 decreased CD7 promoter activity, a C-terminal deletion form of Twist2 reversed its inhibition, suggesting an important role of the C-terminus in CD7 regulation. In addition, CD7 expression was enhanced by histone deacetylase inhibitors such as trichostatin A and sodium butyrate, which indicates that Twist2 might be one of candidate factors involved in histone deacetylation. Based on these results, we conclude that upregulation of Twist2 increases the resistance to galectin-1-mediated-apoptosis, which may have significant implications for the progression of some T-cells into tumors such as Sezary cells.

Keywords CD7, galectin-1-mediated apoptosis, histone deacetylation, Twist2

Article

Research Article

Mol. Cells 2009; 28(6): 553-558

Published online December 31, 2009 https://doi.org/10.1007/s10059-009-0150-8

Copyright © The Korean Society for Molecular and Cellular Biology.

Twist2 Regulates CD7 Expression and
Galectin-1-Induced Apoptosis in Mature T-Cells

Han Seok Koh, Changjin Lee, Kwang Soo Lee, Eun Jung Park, Rho H. Seong, Seokmann Hong, and Sung Ho Jeon

Received: August 5, 2009; Revised: September 15, 2009; Accepted: September 17, 2009

Abstract

In the periphery, a galectin-1 receptor, CD7, plays crucial roles in galectin-1-mediated apoptosis of activated T-cells as well as progression of T-lymphoma. Previously, we demonstrated that NF-κB downregulated CD7 gene expression through the p38 MAPK pathway in developing immature thymocytes. However, its regulatory pathway is not well understood in functional mature T-cells. Here, we show that CD7 expression was downregulated by Twist2 in Jurkat cells, a human acute T-cell lymphoma cell line, and in EL4 cells, a mature mur-ine T-cell lymphoma cell line. Furthermore, ectopic expression of Twist2 in Jurkat cells reduced galectin-1-induced apoptosis. While full-length Twist2 decreased CD7 promoter activity, a C-terminal deletion form of Twist2 reversed its inhibition, suggesting an important role of the C-terminus in CD7 regulation. In addition, CD7 expression was enhanced by histone deacetylase inhibitors such as trichostatin A and sodium butyrate, which indicates that Twist2 might be one of candidate factors involved in histone deacetylation. Based on these results, we conclude that upregulation of Twist2 increases the resistance to galectin-1-mediated-apoptosis, which may have significant implications for the progression of some T-cells into tumors such as Sezary cells.

Keywords: CD7, galectin-1-mediated apoptosis, histone deacetylation, Twist2

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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