Mol. Cells 2009; 28(6): 521-527
Published online November 19, 2009
https://doi.org/10.1007/s10059-009-0158-0
© The Korean Society for Molecular and Cellular Biology
Previously, we have reported tissue- and stage-specific expression of miR-372 in human embryonic stem cells and so far, not many reports speculate the function of this microRNA (miRNA). In this study, we screened various human cancer cell lines including gastric cancer cell lines and found first time that miR-372 is expressed only in AGS human gastric adenocarcinoma cell line. Inhibition of miR-372 using antisense miR-372 oligonucleotide (AS-miR-372) suppressed proliferation, arrested the cell cycle at G2/M phase, and increased apoptosis of AGS cells. Furthermore, AS-miR-372 treatment increased expression of LATS2, while over-expression of miR-372 decreased luciferase reporter activity driven by the 3' untranslated region (3' UTR) of LATS2 mRNA. Over-expression of LATS2 induced changes in AGS cells similar to those in AGS cells treated with AS-miR-372. Taken together, these findings demonstrate an oncogenic role for miR-372 in controlling cell growth, cell cycle, and apoptosis through down-regulation of a tumor suppressor gene, LATS2.
Keywords AGS gastric cancer cell line, apoptosis, cell cycle, G2-M transition, LATS2, miR-372
Mol. Cells 2009; 28(6): 521-527
Published online December 31, 2009 https://doi.org/10.1007/s10059-009-0158-0
Copyright © The Korean Society for Molecular and Cellular Biology.
Wha Ja Cho, Jeong Min Shin, Jong Soo Kim, Man Ryul Lee, Ki Sung Hong, Jun-Ho Lee,
Kyoung Hwa Koo, Jeong Woo Park, and Kye-Seong Kim
Previously, we have reported tissue- and stage-specific expression of miR-372 in human embryonic stem cells and so far, not many reports speculate the function of this microRNA (miRNA). In this study, we screened various human cancer cell lines including gastric cancer cell lines and found first time that miR-372 is expressed only in AGS human gastric adenocarcinoma cell line. Inhibition of miR-372 using antisense miR-372 oligonucleotide (AS-miR-372) suppressed proliferation, arrested the cell cycle at G2/M phase, and increased apoptosis of AGS cells. Furthermore, AS-miR-372 treatment increased expression of LATS2, while over-expression of miR-372 decreased luciferase reporter activity driven by the 3' untranslated region (3' UTR) of LATS2 mRNA. Over-expression of LATS2 induced changes in AGS cells similar to those in AGS cells treated with AS-miR-372. Taken together, these findings demonstrate an oncogenic role for miR-372 in controlling cell growth, cell cycle, and apoptosis through down-regulation of a tumor suppressor gene, LATS2.
Keywords: AGS gastric cancer cell line, apoptosis, cell cycle, G2-M transition, LATS2, miR-372
Ziwei Chang, Ming Lu, Sung-Min Park, Hyun-Kyung Park, Ho Sung Kang, Youngshang Pak, and Jang-Su Park
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