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Mol. Cells 2009; 28(1): 57-65

Published online July 31, 2009

https://doi.org/10.1007/s10059-009-0101-4

© The Korean Society for Molecular and Cellular Biology

Suppression of the ER-Localized AAA ATPase NgCDC48 Inhibits Tobacco Growth and Development

Hansol Bae, Soo Min Choi, Seong Wook Yang, Hyun-Sook Pai, and Woo Taek Kim

Received: May 11, 2009; Revised: May 29, 2009; Accepted: June 3, 2009

Abstract

CDC48 is a member of the AAA ATPase superfamily. Yeast CDC48 and its mammalian homolog p97 are impli-cated in diverse cellular processes, including mitosis, membrane fusion, and ubiquitin-dependent protein degradation. However, the cellular functions of plant CDC48 proteins are largely unknown. In the present study, we performed virus-induced gene silencing (VIGS) screening and found that silencing of a gene encoding a tobacco CDC48 homolog, NgCDC48, resulted in severe abnormalities in leaf and shoot development in tobacco. Furthermore, transgenic tobacco plants (35S:anti-NgCDC48), in which the NgCDC48 gene was suppressed using the antisense RNA method, exhibited severely aberrant development of both vegetative and reproductive organs, resulting in arrested shoot and leaf growth and sterile flowers. Approximately 57-83% of 35S:anti-NgCDC48 plants failed to develop mature organs and died at early stage of development. Scanning electron microscopy showed that both adaxial and abaxial epidermal pavement cells in antisense transgenic leaves were significantly smaller and more numerous than those in wild type leaves. These results indicate that NgCDC48 is critically involved in cell growth and development of tobacco plants. An in vivo targeting experiment revealed that NgCDC48 resides in the endoplasmic reticulum (ER) in tobacco protoplasts. We consider the tantalizing possibility that CDC48-mediated degradation of an as-yet unidentified protein(s) in the ER might be a critical step for cell growth and expansion in tobacco leaves.

Keywords AAA ATPase CDC48 homolog, antisense suppression, growth and development, tobacco (Nicotiana tabacum), virus-induced gene silenc-ing

Article

Research Article

Mol. Cells 2009; 28(1): 57-65

Published online July 31, 2009 https://doi.org/10.1007/s10059-009-0101-4

Copyright © The Korean Society for Molecular and Cellular Biology.

Suppression of the ER-Localized AAA ATPase NgCDC48 Inhibits Tobacco Growth and Development

Hansol Bae, Soo Min Choi, Seong Wook Yang, Hyun-Sook Pai, and Woo Taek Kim

Received: May 11, 2009; Revised: May 29, 2009; Accepted: June 3, 2009

Abstract

CDC48 is a member of the AAA ATPase superfamily. Yeast CDC48 and its mammalian homolog p97 are impli-cated in diverse cellular processes, including mitosis, membrane fusion, and ubiquitin-dependent protein degradation. However, the cellular functions of plant CDC48 proteins are largely unknown. In the present study, we performed virus-induced gene silencing (VIGS) screening and found that silencing of a gene encoding a tobacco CDC48 homolog, NgCDC48, resulted in severe abnormalities in leaf and shoot development in tobacco. Furthermore, transgenic tobacco plants (35S:anti-NgCDC48), in which the NgCDC48 gene was suppressed using the antisense RNA method, exhibited severely aberrant development of both vegetative and reproductive organs, resulting in arrested shoot and leaf growth and sterile flowers. Approximately 57-83% of 35S:anti-NgCDC48 plants failed to develop mature organs and died at early stage of development. Scanning electron microscopy showed that both adaxial and abaxial epidermal pavement cells in antisense transgenic leaves were significantly smaller and more numerous than those in wild type leaves. These results indicate that NgCDC48 is critically involved in cell growth and development of tobacco plants. An in vivo targeting experiment revealed that NgCDC48 resides in the endoplasmic reticulum (ER) in tobacco protoplasts. We consider the tantalizing possibility that CDC48-mediated degradation of an as-yet unidentified protein(s) in the ER might be a critical step for cell growth and expansion in tobacco leaves.

Keywords: AAA ATPase CDC48 homolog, antisense suppression, growth and development, tobacco (Nicotiana tabacum), virus-induced gene silenc-ing

Mol. Cells
Jun 30, 2022 Vol.45 No.6, pp. 353~434
COVER PICTURE
ERα is modified by UFM1 and this modification (ufmylation) plays a crucial role in promoting the stability of ERα and breast cancer development. However, when ERα is deufmylated and then ubiquitinated, it disappears by proteasome-mediated degradation (Yoo et al., pp. 425-434).

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