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Mol. Cells 2009; 28(1): 31-36

Published online July 31, 2009

https://doi.org/10.1007/s10059-009-0097-9

© The Korean Society for Molecular and Cellular Biology

Centrobin/Nip2 Expression In Vivo Suggests Its Involvement in Cell Proliferation

Jungmin Lee, Sunmi Kim, Yeontae Jeong, and Kunsoo Rhee

Received: March 25, 2009; Revised: April 29, 2009; Accepted: May 6, 2009

Abstract

Centrobin/Nip2 was initially identified as a centrosome protein that is critical for centrosome duplication and spindle assembly. In the present study, we determined the expression and subcellular localization of centrobin in selected mouse tissues. Immunoblot analysis revealed that the centrobin-specific band of 100 kDa was detected in all tissues tested but most abundantly in the thymus, spleen and testis. In the testis, centrobin was localized at the centrosomes of spermatocytes and early round sper-matids, but no specific signal was detected in late round spermatids and elongated spermatids. Our results also revealed that the centrosome duplication occurs at inter-phase of the second meiotic division of the mouse male germ cells. The centrobin protein was more abundant in the mitotically active ovarian follicular cells and thymic cortex cells than in non-proliferating corpus luteal cells and thymic medullary cells. The expression pattern of centrobin suggests that the biological functions of centrobin are related to cell proliferation. Consistent with the proposal, we observed reduction of the centrobin levels when NIH3T3 became quiescent in the serum-starved culture conditions. However, a residual amount of centrobin was also detected at the centrosomes of the resting cells, suggesting its role for maintaining integrity of the centrosome, especially of the daughter centriole in the cells.

Keywords cell cycle, centrobin, centrosome, meiosis, NIP2, spermatogenesis

Article

Research Article

Mol. Cells 2009; 28(1): 31-36

Published online July 31, 2009 https://doi.org/10.1007/s10059-009-0097-9

Copyright © The Korean Society for Molecular and Cellular Biology.

Centrobin/Nip2 Expression In Vivo Suggests Its Involvement in Cell Proliferation

Jungmin Lee, Sunmi Kim, Yeontae Jeong, and Kunsoo Rhee

Received: March 25, 2009; Revised: April 29, 2009; Accepted: May 6, 2009

Abstract

Centrobin/Nip2 was initially identified as a centrosome protein that is critical for centrosome duplication and spindle assembly. In the present study, we determined the expression and subcellular localization of centrobin in selected mouse tissues. Immunoblot analysis revealed that the centrobin-specific band of 100 kDa was detected in all tissues tested but most abundantly in the thymus, spleen and testis. In the testis, centrobin was localized at the centrosomes of spermatocytes and early round sper-matids, but no specific signal was detected in late round spermatids and elongated spermatids. Our results also revealed that the centrosome duplication occurs at inter-phase of the second meiotic division of the mouse male germ cells. The centrobin protein was more abundant in the mitotically active ovarian follicular cells and thymic cortex cells than in non-proliferating corpus luteal cells and thymic medullary cells. The expression pattern of centrobin suggests that the biological functions of centrobin are related to cell proliferation. Consistent with the proposal, we observed reduction of the centrobin levels when NIH3T3 became quiescent in the serum-starved culture conditions. However, a residual amount of centrobin was also detected at the centrosomes of the resting cells, suggesting its role for maintaining integrity of the centrosome, especially of the daughter centriole in the cells.

Keywords: cell cycle, centrobin, centrosome, meiosis, NIP2, spermatogenesis

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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