TOP

Research Article

Split Viewer

Mol. Cells 2008; 26(4): 380-386

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Identification of Osteogenic Purmorphamine Derivatives

Sung-Jin Lee, Hak-Kyo Lee, Sung Yun Cho, Joong-Kwon Choi, Hea Kyeong Shin, Eun-Jung Kwak, Mi-Ran Cho, Hye-Ryun Kim, Seung-Ryol Kim, Yong-Min Kim, Kyoung-Jin Park and Joong-Kook Choi

Abstract

During embryonic and cancer development, the Hedgehog family of proteins, including Sonic Hedgehog, play an important role by relieving the inhibition of Smo by Ptc, thus activating the Smo signaling cascade. Recently, a purine compound, purmorphamine, has been reported to target the Hedgehog signaling pathway by interacting with Smo. Interestingly, both Sonic Hedgehog and purmorphamine were found to promote the osteogenic differentiation of mouse chondroprogenitor cells. However, there is insufficient information as to how the activation of this seemingly unrelated signaling pathway, either by Sonic Hedgehog or purmorphamine, contributes to osteogenesis.
Using alkaline phosphatase assays, we screened 125 purmorphamine derivatives from the Korea Chemical Bank for effects on the differentiation of preosteoblast C2C12 cells. Here, we report that two purine derivatives modulate ALP activity as well as the expression of genes whose expression is known or suggested to be involved in osteogenesis.

Keywords C2C12 preosteoblasts, osteogenesis, osteogenesis, purmorphamine, purmorphamine, Sonic hedgehog, alkaline phosphatase assay, C2C12 preosteoblasts

Article

Research Article

Mol. Cells 2008; 26(4): 380-386

Published online October 31, 2008

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of Osteogenic Purmorphamine Derivatives

Sung-Jin Lee, Hak-Kyo Lee, Sung Yun Cho, Joong-Kwon Choi, Hea Kyeong Shin, Eun-Jung Kwak, Mi-Ran Cho, Hye-Ryun Kim, Seung-Ryol Kim, Yong-Min Kim, Kyoung-Jin Park and Joong-Kook Choi

Abstract

During embryonic and cancer development, the Hedgehog family of proteins, including Sonic Hedgehog, play an important role by relieving the inhibition of Smo by Ptc, thus activating the Smo signaling cascade. Recently, a purine compound, purmorphamine, has been reported to target the Hedgehog signaling pathway by interacting with Smo. Interestingly, both Sonic Hedgehog and purmorphamine were found to promote the osteogenic differentiation of mouse chondroprogenitor cells. However, there is insufficient information as to how the activation of this seemingly unrelated signaling pathway, either by Sonic Hedgehog or purmorphamine, contributes to osteogenesis.
Using alkaline phosphatase assays, we screened 125 purmorphamine derivatives from the Korea Chemical Bank for effects on the differentiation of preosteoblast C2C12 cells. Here, we report that two purine derivatives modulate ALP activity as well as the expression of genes whose expression is known or suggested to be involved in osteogenesis.

Keywords: C2C12 preosteoblasts, osteogenesis, osteogenesis, purmorphamine, purmorphamine, Sonic hedgehog, alkaline phosphatase assay, C2C12 preosteoblasts

Mol. Cells
Sep 30, 2023 Vol.46 No.9, pp. 527~572
COVER PICTURE
Chronic obstructive pulmonary disease (COPD) is marked by airspace enlargement (emphysema) and small airway fibrosis, leading to airflow obstruction and eventual respiratory failure. Shown is a microphotograph of hematoxylin and eosin (H&E)-stained histological sections of the enlarged alveoli as an indicator of emphysema. Piao et al. (pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD.

Share this article on

  • line

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download