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Mol. Cells 2008; 26(4): 362-367

Published online October 31, 2008

© The Korean Society for Molecular and Cellular Biology

Identification of a Cryptic Type III Polyketide Synthase (1,3,6,8-Tetrahydroxynaphthalene Synthase) from Streptomyces peucetius ATCC 27952

Gopal Prasad Ghimire, Tae-Jin Oh, Kwangkyoung Liou and Jae Kyung Sohng

Abstract

We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydrox-ynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).

Keywords chalcone synthase, heterologous expression, Streptomyces peucetius, 1,3,6,8-tetrahydroxynaphthalene synthase

Article

Research Article

Mol. Cells 2008; 26(4): 362-367

Published online October 31, 2008

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of a Cryptic Type III Polyketide Synthase (1,3,6,8-Tetrahydroxynaphthalene Synthase) from Streptomyces peucetius ATCC 27952

Gopal Prasad Ghimire, Tae-Jin Oh, Kwangkyoung Liou and Jae Kyung Sohng

Abstract

We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydrox-ynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).

Keywords: chalcone synthase, heterologous expression, Streptomyces peucetius, 1,3,6,8-tetrahydroxynaphthalene synthase

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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