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Mol. Cells 2008; 26(2): 181-185

Published online August 31, 2008

© The Korean Society for Molecular and Cellular Biology

Calcitonin Gene-related Peptide Suppresses Pacemaker Currents by Nitric Oxide/cGMP-dependent Activation of ATP-sensitive K(+) Channels in Cultured Interstitial Cells of Cajal from the Mouse Small Intestine

Seok Choi, Shankar Prasad Parajuli, Cheol Ho Yeum, Chan Guk Park, Man Yoo Kim, Young Dae Kim, Kyoung Hun Cha, Young Bong Park, Jong Seong Park, Han Seong Jeong and Jae Yeoul Jun

Abstract

The effects of calcitonin gene-related peptide (CGRP) on pacemaker currents in cultured interstitial cells of Cajal (ICC) from the mouse small intestine were investigated using the whole-cell patch clamp technique at 30?C. Under voltage clamping at a holding potential of -70 mV, CGRP decreased the amplitude and frequency of pacemaker currents and activated outward resting currents. These effects were blocked by intracellular GDP?S, a G-protein inhibitor and glibenclamide, a specific ATP-sensitive K+ channels blocker. During current clamping, CGRP hyperpolarized the membrane and this effect was antagonized by glibenclamide. Pretreatment with SQ-22536 (an adenylate cyclase inhibitor) or naproxen (a cyclooxygenase inhibitor) did not block the CGRP-induced effects, whereas pretreatment with ODQ (a guanylate cyclase inhibitor) or L-NAME (an inhibitor of nitric oxide synthase) did. In conclusion, CGRP inhibits pacemaker currents in ICC by generating nitric oxide via G-protein activation and so activating ATP-sensitive K+ channels. Nitric oxide- and guanylate cyclase- dependent pathways are involved in these effects.

Keywords ATP-Sensitive K+ channels, calcitonin gene-related peptide, interstitial cells of cajal, nitric oxide, pacemaker currents, small intestine

Article

Research Article

Mol. Cells 2008; 26(2): 181-185

Published online August 31, 2008

Copyright © The Korean Society for Molecular and Cellular Biology.

Calcitonin Gene-related Peptide Suppresses Pacemaker Currents by Nitric Oxide/cGMP-dependent Activation of ATP-sensitive K(+) Channels in Cultured Interstitial Cells of Cajal from the Mouse Small Intestine

Seok Choi, Shankar Prasad Parajuli, Cheol Ho Yeum, Chan Guk Park, Man Yoo Kim, Young Dae Kim, Kyoung Hun Cha, Young Bong Park, Jong Seong Park, Han Seong Jeong and Jae Yeoul Jun

Abstract

The effects of calcitonin gene-related peptide (CGRP) on pacemaker currents in cultured interstitial cells of Cajal (ICC) from the mouse small intestine were investigated using the whole-cell patch clamp technique at 30?C. Under voltage clamping at a holding potential of -70 mV, CGRP decreased the amplitude and frequency of pacemaker currents and activated outward resting currents. These effects were blocked by intracellular GDP?S, a G-protein inhibitor and glibenclamide, a specific ATP-sensitive K+ channels blocker. During current clamping, CGRP hyperpolarized the membrane and this effect was antagonized by glibenclamide. Pretreatment with SQ-22536 (an adenylate cyclase inhibitor) or naproxen (a cyclooxygenase inhibitor) did not block the CGRP-induced effects, whereas pretreatment with ODQ (a guanylate cyclase inhibitor) or L-NAME (an inhibitor of nitric oxide synthase) did. In conclusion, CGRP inhibits pacemaker currents in ICC by generating nitric oxide via G-protein activation and so activating ATP-sensitive K+ channels. Nitric oxide- and guanylate cyclase- dependent pathways are involved in these effects.

Keywords: ATP-Sensitive K+ channels, calcitonin gene-related peptide, interstitial cells of cajal, nitric oxide, pacemaker currents, small intestine

Mol. Cells
May 31, 2022 Vol.45 No.5, pp. 273~352
COVER PICTURE
Fe2+ ion depletion-induced expression of BΔGFP at the early stage of leaf development (Choi et al., pp. 294-305).

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