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Mol. Cells 2005; 20(2): 219-227

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Identification of Genes Differentially Expressed in Wild Type and Purkinje Cell Degeneration Mice

Rui Xiao, Youngsook Park, Vijaya R. Dirisala, Ya-Ping Zhang, Sang June Um, Hoon Taek Lee, Chankyu Park

Abstract

Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.

Keywords Agtpbp1; Cerebellum; Gene Expression; Microarray; pcd; Purkinje Cell Degeneration

Article

Research Article

Mol. Cells 2005; 20(2): 219-227

Published online October 31, 2005

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of Genes Differentially Expressed in Wild Type and Purkinje Cell Degeneration Mice

Rui Xiao, Youngsook Park, Vijaya R. Dirisala, Ya-Ping Zhang, Sang June Um, Hoon Taek Lee, Chankyu Park

Abstract

Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.

Keywords: Agtpbp1, Cerebellum, Gene Expression, Microarray, pcd, Purkinje Cell Degeneration

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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Molecules and Cells

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