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Mol. Cells 2005; 20(2): 219-227

Published online October 31, 2005

© The Korean Society for Molecular and Cellular Biology

Identification of Genes Differentially Expressed in Wild Type and Purkinje Cell Degeneration Mice

Rui Xiao, Youngsook Park, Vijaya R. Dirisala, Ya-Ping Zhang, Sang June Um, Hoon Taek Lee, Chankyu Park

Abstract

Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.

Keywords Agtpbp1; Cerebellum; Gene Expression; Microarray; pcd; Purkinje Cell Degeneration

Article

Research Article

Mol. Cells 2005; 20(2): 219-227

Published online October 31, 2005

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of Genes Differentially Expressed in Wild Type and Purkinje Cell Degeneration Mice

Rui Xiao, Youngsook Park, Vijaya R. Dirisala, Ya-Ping Zhang, Sang June Um, Hoon Taek Lee, Chankyu Park

Abstract

Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.

Keywords: Agtpbp1, Cerebellum, Gene Expression, Microarray, pcd, Purkinje Cell Degeneration

Mol. Cells
Aug 31, 2022 Vol.45 No.8, pp. 513~602
COVER PICTURE
Cryo-EM structure of human porphyrin transporter ABCB6 (main figure) shows that binding of hemin (inset, magenta) in concert with two glutathione molecules (cyan) primes ABCB6 for high ATP turnover (Kim et al., pp. 575-587).

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