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Mol. Cells 2005; 20(2): 219-227

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Identification of Genes Differentially Expressed in Wild Type and Purkinje Cell Degeneration Mice

Rui Xiao, Youngsook Park, Vijaya R. Dirisala, Ya-Ping Zhang, Sang June Um, Hoon Taek Lee, Chankyu Park

Abstract

Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.

Keywords Agtpbp1; Cerebellum; Gene Expression; Microarray; pcd; Purkinje Cell Degeneration

Article

Research Article

Mol. Cells 2005; 20(2): 219-227

Published online October 31, 2005

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of Genes Differentially Expressed in Wild Type and Purkinje Cell Degeneration Mice

Rui Xiao, Youngsook Park, Vijaya R. Dirisala, Ya-Ping Zhang, Sang June Um, Hoon Taek Lee, Chankyu Park

Abstract

Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.

Keywords: Agtpbp1, Cerebellum, Gene Expression, Microarray, pcd, Purkinje Cell Degeneration

Mol. Cells
Sep 30, 2023 Vol.46 No.9, pp. 527~572
COVER PICTURE
Chronic obstructive pulmonary disease (COPD) is marked by airspace enlargement (emphysema) and small airway fibrosis, leading to airflow obstruction and eventual respiratory failure. Shown is a microphotograph of hematoxylin and eosin (H&E)-stained histological sections of the enlarged alveoli as an indicator of emphysema. Piao et al. (pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD.

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