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Mol. Cells 2005; 20(1): 30-34
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
Identification of a Rice Gene (Bph 1) Conferring Resistance to Brown Planthopper (Nilaparvata Iugens Stal) Using STS Markers
This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.
Keywords Bph1; Marker Assisted Selection; Oryza sativa L.; STS Marker
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Research Article
Mol. Cells 2005; 20(1): 30-34
Published online August 31, 2005
Copyright © The Korean Society for Molecular and Cellular Biology.
Identification of a Rice Gene (Bph 1) Conferring Resistance to Brown Planthopper (Nilaparvata Iugens Stal) Using STS Markers
Suk-Man Kim, Jae-Keun Sohn
Abstract
This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.
Keywords: Bph1, Marker Assisted Selection, Oryza sativa L., STS Marker
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