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Mol. Cells 2012; 34(2): 159-164
Published online July 18, 2012
https://doi.org/10.1007/s10059-012-0055-9
© The Korean Society for Molecular and Cellular Biology
Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide
Correspondence to : *Correspondence: jrlee@ewha.ac.kr
We studied the role of a RhoA-specific guanine nucleo-tide exchange factor (p190RhoGEF) in dendritic cells (DCs), using transgenic (TG) mice that over-express a full gene of p190RhoGEF under the control of an invariant chain promoter. TG mice lacked localization of activated DCs to the T cell zone in the spleen and had reduced serum levels of IL-6 in response to lipopolysaccharide (LPS) injection. DCs from these mice also showed reduced surface expression of CD86, CD40, and CD205, but not MHCII, as well as a reduced capability to uptake antigen. Moreover, chemokine- driven migration and secretion of IL-6, but not of IL-12, were impaired after LPS-stimulation of TG DCs. Collectively, these results suggest that over-expressing p190RhoGEF nega-tively regulates conventional DC function in response to bacterial LPS infection.
Keywords dendritic cells, lipopolysaccharides, p190RhoGEF
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Research Article
Mol. Cells 2012; 34(2): 159-164
Published online August 31, 2012 https://doi.org/10.1007/s10059-012-0055-9
Copyright © The Korean Society for Molecular and Cellular Biology.
Over-Expression of a RhoA-Specific Guanine Nucleotide Exchange Factor, p190RhoGEF, in Mouse Dendritic Cells Negatively Regulates Cellular Responses to Bacterial Lipopolysaccharide
Hee Jung Seul1, Yu Ri Ahn1, Hyeon Myeong Song1, Yun Jung Ha1, and Jong Ran Lee1,2,3,*
1Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea, 2Department of Life Science, College of Natural Sciences, Ewha Womans University, Seoul 120-750, Korea, 3Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Seoul 120-750, Korea
Correspondence to:*Correspondence: jrlee@ewha.ac.kr
Abstract
We studied the role of a RhoA-specific guanine nucleo-tide exchange factor (p190RhoGEF) in dendritic cells (DCs), using transgenic (TG) mice that over-express a full gene of p190RhoGEF under the control of an invariant chain promoter. TG mice lacked localization of activated DCs to the T cell zone in the spleen and had reduced serum levels of IL-6 in response to lipopolysaccharide (LPS) injection. DCs from these mice also showed reduced surface expression of CD86, CD40, and CD205, but not MHCII, as well as a reduced capability to uptake antigen. Moreover, chemokine- driven migration and secretion of IL-6, but not of IL-12, were impaired after LPS-stimulation of TG DCs. Collectively, these results suggest that over-expressing p190RhoGEF nega-tively regulates conventional DC function in response to bacterial LPS infection.
Keywords: dendritic cells, lipopolysaccharides, p190RhoGEF
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