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Mol. Cells 2002; 13(3): 413-418
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
Karyotype Analysis of a Korean Cucumber Cultivar (Cucumis sativus L. cv. Winter Long) Using C-banding and Bicolor Fluorescence in situ Hybridization
An intensive karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) was car-ried out with three different methods. These included Feulgen staining, Giemsa C-banding, and fluorescence in situ hybridization (FISH). The mitotic chromosomes of the cucumber (2n = 2x = 14) were characterized, based on the length and arm ratio values. A C-banding analysis showed dark stains on the centromeric, te-lomeric, and intercalary regions of the chromosomes, except that chromosome 2 had a heavy staining in the long arm. Bicolor FISH, using 45S and 5S rDNA probes, provided additional information to identify cucumber chromosomes. The signals for 45S rDNA were detected on the pericentromeric regions of chro-mosomes 1, 2, and 4. The signals for 5S rDNA were on the short arm of chromosome 5. Similar band patterns (as the C-banding) were observed when the chromo-somes were counter-stained with 4?6-diamidino-2-phenyoindole (DAPI). The data implied that the karyotype of the Korean cucumber cultivar is peculiar and different from previous reports.
Keywords C-banding, Bicolor FISH, Karyo, Cucumis sativus L.
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Research Article
Mol. Cells 2002; 13(3): 413-418
Published online June 30, 2002
Copyright © The Korean Society for Molecular and Cellular Biology.
Karyotype Analysis of a Korean Cucumber Cultivar (Cucumis sativus L. cv. Winter Long) Using C-banding and Bicolor Fluorescence in situ Hybridization
Dal-Hoe Koo, Yoonkang Hur, Dong-Chun Jin, Jae-Wook Bang
Abstract
An intensive karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) was car-ried out with three different methods. These included Feulgen staining, Giemsa C-banding, and fluorescence in situ hybridization (FISH). The mitotic chromosomes of the cucumber (2n = 2x = 14) were characterized, based on the length and arm ratio values. A C-banding analysis showed dark stains on the centromeric, te-lomeric, and intercalary regions of the chromosomes, except that chromosome 2 had a heavy staining in the long arm. Bicolor FISH, using 45S and 5S rDNA probes, provided additional information to identify cucumber chromosomes. The signals for 45S rDNA were detected on the pericentromeric regions of chro-mosomes 1, 2, and 4. The signals for 5S rDNA were on the short arm of chromosome 5. Similar band patterns (as the C-banding) were observed when the chromo-somes were counter-stained with 4?6-diamidino-2-phenyoindole (DAPI). The data implied that the karyotype of the Korean cucumber cultivar is peculiar and different from previous reports.
Keywords: C-banding, Bicolor FISH, Karyo, Cucumis sativus L.
