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Mol. Cells 1994; 4(3): 355-360

Published online September 30, 1994

© The Korean Society for Molecular and Cellular Biology

Expression of Human Lactoferrin in Milk of Transgenic Mice Using Bovine β-casein/Human Lactoferrin cDNA Fusion Gene

Sun Jung Kim, Yong-Yeon Cho, Ko-Woon Lee, Dae-Yeul Yu, Chul-Sang Lee,
Yong-Mahn Han and Kyung-Kwang Lee

Abstract

Transgenic mice were developed which expressed human lactoferrin (hLF) in the mammary gland. This was achieved by using regulatory elements of bovine β-casein gene, rabbit β-globin intron II, and SV40 polyadenylation signal to control the expression of hLF cDNA. Twenty lines of transgenic mice were produced. Milk could be collected from four lines of transgenic mice. In two of them hLF was present in the milk at concentrations of 150~200 μg/ml. Mammary-specific hLF expression was not correlated to the transgene copy number. hLF RNA was only detected in the mammary gland of transgenic mice. The expressed RNA was correctly spliced at the exon/intron junctions. From these results, it may be concluded that the recombinant expression vector developed in this study is a good candidate to control high levels of foreign gene expression in the mammary gland of transgenic animals.

Article

Research Article

Mol. Cells 1994; 4(3): 355-360

Published online September 30, 1994

Copyright © The Korean Society for Molecular and Cellular Biology.

Expression of Human Lactoferrin in Milk of Transgenic Mice Using Bovine β-casein/Human Lactoferrin cDNA Fusion Gene

Sun Jung Kim, Yong-Yeon Cho, Ko-Woon Lee, Dae-Yeul Yu, Chul-Sang Lee,
Yong-Mahn Han and Kyung-Kwang Lee

Abstract

Transgenic mice were developed which expressed human lactoferrin (hLF) in the mammary gland. This was achieved by using regulatory elements of bovine β-casein gene, rabbit β-globin intron II, and SV40 polyadenylation signal to control the expression of hLF cDNA. Twenty lines of transgenic mice were produced. Milk could be collected from four lines of transgenic mice. In two of them hLF was present in the milk at concentrations of 150~200 μg/ml. Mammary-specific hLF expression was not correlated to the transgene copy number. hLF RNA was only detected in the mammary gland of transgenic mice. The expressed RNA was correctly spliced at the exon/intron junctions. From these results, it may be concluded that the recombinant expression vector developed in this study is a good candidate to control high levels of foreign gene expression in the mammary gland of transgenic animals.

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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