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Mol. Cells 2008; 26(1): 18-25

Published online July 31, 2008

© The Korean Society for Molecular and Cellular Biology

Suppression of Arsenic Trioxide-induced Apoptosis in HeLa Cells by N-Acetylcysteine

Yong Hwan Han, Sung Zoo Kim, Suhn Hee Kim and Woo Hyun Park

Abstract

Arsenic trioxide (ATO) can affect many biological functions such as apoptosis and differentiation in various cells. We investigated the involvement of ROS and GSH in ATO-induced HeLa cell death using ROS scavengers, especially N-acetylcysteine (NAC). ATO increased intracellular O2?- levels and reduced intracellular GSH content. The ROS scavengers, Tempol, Tiron and Trimetazidine, did not significantly reduce levels of ROS or GSH depletion in ATO-treated HeLa cells. Nor did they reduce the apoptosis induced by ATO. In contrast, treatment with NAC reduced ROS levels and GSH depletion in the ATO-treated HeLa cells and prevented ATO-induced apoptosis. Treatment with exogenous SOD and catalase reduced the depletion of GSH content in ATO-treated cells. Catalase strongly protected the cells from ATO-induced apoptosis. In addition, treatment with SOD, catalase and NAC slightly inhibited the G1 phase accumulation induced by ATO. In conclusion, NAC protects HeLa cells from apoptosis induced by ATO by up-regulating intracellular GSH content and partially reducing the pro-duction of O2?-.

Keywords Apoptosis, Arsenic trioxide, GSH, HeLa, ROS, ROS Scavenger

Article

Research Article

Mol. Cells 2008; 26(1): 18-25

Published online July 31, 2008

Copyright © The Korean Society for Molecular and Cellular Biology.

Suppression of Arsenic Trioxide-induced Apoptosis in HeLa Cells by N-Acetylcysteine

Yong Hwan Han, Sung Zoo Kim, Suhn Hee Kim and Woo Hyun Park

Abstract

Arsenic trioxide (ATO) can affect many biological functions such as apoptosis and differentiation in various cells. We investigated the involvement of ROS and GSH in ATO-induced HeLa cell death using ROS scavengers, especially N-acetylcysteine (NAC). ATO increased intracellular O2?- levels and reduced intracellular GSH content. The ROS scavengers, Tempol, Tiron and Trimetazidine, did not significantly reduce levels of ROS or GSH depletion in ATO-treated HeLa cells. Nor did they reduce the apoptosis induced by ATO. In contrast, treatment with NAC reduced ROS levels and GSH depletion in the ATO-treated HeLa cells and prevented ATO-induced apoptosis. Treatment with exogenous SOD and catalase reduced the depletion of GSH content in ATO-treated cells. Catalase strongly protected the cells from ATO-induced apoptosis. In addition, treatment with SOD, catalase and NAC slightly inhibited the G1 phase accumulation induced by ATO. In conclusion, NAC protects HeLa cells from apoptosis induced by ATO by up-regulating intracellular GSH content and partially reducing the pro-duction of O2?-.

Keywords: Apoptosis, Arsenic trioxide, GSH, HeLa, ROS, ROS Scavenger

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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