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Mol. Cells 2007; 23(2): 138-144

Published online April 30, 2007

© The Korean Society for Molecular and Cellular Biology

Lactosylceramide α2,3-Sialyltransferase Is Induced Via a PKC/ERK/CREB-dependent Pathway in K562 Human Leukemia Cells

Hee-Jung Choi, Young-Guk Park, Cheorl-Ho Kim

Abstract

Previously we showed that the human GM3 synthase gene was expressed during the induction of megakaryocytic differentiation in human leukemia K562 cells by phorbol 12-myristate 13-acetate (PMA). In this study we found that treatment of PMA-induced K562 cells with G?6976, a specific inhibitor of PKC, and U0126, an inhibitor of the extracellular signal-regulated kinase (ERK) reduced expression of GM3 synthase, whereas wortmannin, an inhibitor of phosphoinositide 3-kinase (PI3K) did not. Moreover, activation of ERK and cAMP response element binding protein (CREB) was prevented by pretreatment with G?6976 and U0126. PMA stimulated the promoter activity of the 5?-flanking region from ?177 to ?83 region of the GM3 synthase gene, and mutation or deletion of a CREB site located around ?143 of the promoter reduced PMA-stimulated promoter activity, as did the inhibitors G?6976 and U0126. Our results demonstrate that induction of GM3 synthase during megakaryocytic differentiation in PMA-stimulated human leukemia K562 cells depends upon the PKC/ERK/CREB pathway.

Keywords CMP-NeuAc:Lactosylceramide α2,3-Sialyltransferase, (GM3 Synthase), Human Leukemia K562 Cells,, PKC/ERK/CREB Pathway

Article

Research Article

Mol. Cells 2007; 23(2): 138-144

Published online April 30, 2007

Copyright © The Korean Society for Molecular and Cellular Biology.

Lactosylceramide α2,3-Sialyltransferase Is Induced Via a PKC/ERK/CREB-dependent Pathway in K562 Human Leukemia Cells

Hee-Jung Choi, Young-Guk Park, Cheorl-Ho Kim

Abstract

Previously we showed that the human GM3 synthase gene was expressed during the induction of megakaryocytic differentiation in human leukemia K562 cells by phorbol 12-myristate 13-acetate (PMA). In this study we found that treatment of PMA-induced K562 cells with G?6976, a specific inhibitor of PKC, and U0126, an inhibitor of the extracellular signal-regulated kinase (ERK) reduced expression of GM3 synthase, whereas wortmannin, an inhibitor of phosphoinositide 3-kinase (PI3K) did not. Moreover, activation of ERK and cAMP response element binding protein (CREB) was prevented by pretreatment with G?6976 and U0126. PMA stimulated the promoter activity of the 5?-flanking region from ?177 to ?83 region of the GM3 synthase gene, and mutation or deletion of a CREB site located around ?143 of the promoter reduced PMA-stimulated promoter activity, as did the inhibitors G?6976 and U0126. Our results demonstrate that induction of GM3 synthase during megakaryocytic differentiation in PMA-stimulated human leukemia K562 cells depends upon the PKC/ERK/CREB pathway.

Keywords: CMP-NeuAc:Lactosylceramide α2,3-Sialyltransferase, (GM3 Synthase), Human Leukemia K562 Cells,, PKC/ERK/CREB Pathway

Mol. Cells
Nov 30, 2021 Vol.44 No.11, pp. 781~860
COVER PICTURE
3D quantitative images of the vesicular structure and the nucleolus using label free optical diffraction tomography (Kim et al., pp. 851-860).

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