Mol. Cells 2020; 43(9): 831-840
Published online September 18, 2020
https://doi.org/10.14348/molcells.2020.0168
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *misunjin@gist.ac.kr
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
The β-class of carbonic anhydrases (β-CAs) are zinc metalloenzymes widely distributed in the fungal kingdom that play essential roles in growth, survival, differentiation, and virulence by catalyzing the reversible interconversion of carbon dioxide (CO2) and bicarbonate (HCO3–). Herein, we report the biochemical and crystallographic characterization of the β-CA CafA from the fungal pathogen Aspergillus fumigatus, the main causative agent of invasive aspergillosis. CafA exhibited apparent in vitro CO2 hydration activity in neutral to weak alkaline conditions, but little activity at acidic pH. The high-resolution crystal structure of CafA revealed a tetramer comprising a dimer of dimers, in which the catalytic zinc ion is tetrahedrally coordinated by three conserved residues (C119, H175, C178) and an acetate anion presumably acquired from the crystallization solution, indicating a freely accessible ″open″ conformation. Furthermore, knowledge of the structure of CafA in complex with the potent inhibitor acetazolamide, together with its functional intolerance of nitrate (NO3–) ions, could be exploited to develop new antifungal agents for the treatment of invasive aspergillosis.
Keywords β-class carbonic anhydrase, Aspergillus fumigatus, CafA, X-ray crystallography, zinc metalloenzyme
Carbonic anhydrases (CAs) are widely distributed zinc metalloenzymes that catalyze the interconversion of carbon dioxide to bicarbonate and a proton (Supuran, 2016). They are currently divided into seven families (α, β, γ, δ, ζ, η, θ), which are evolutionarily unrelated in amino acid sequence and structure (Del Prete et al., 2014a; 2014b; Iverson et al., 2000; Kikutani et al., 2016; Meldrum and Roughton, 1933; Mitsuhashi et al., 2000; Xu et al., 2008). Of the different classes, α- and β-CAs have been well-characterized biophysically and structurally. α-CAs are found in mammals, fungi, prokaryotes, and plants. All known bacterial α-CAs are dimers (Kim et al., 2019b; Kimber and Pai, 2000), while most mammalian CAs are monomers, with some also possessing dimeric α-CA forms (Alterio et al., 2009; Pilka et al., 2012; Whittington et al., 2001). α-CAs have a catalytic zinc ion coordinated by three conserved histidine residues and a water molecule (or hydroxide ion) in their active sites (Nair et al., 1995). Extensive structural and biochemical analysis has led to the two-step CO2 hydration model for the α-CA family (Silverman and Lindskog, 1988). In this model, the first step is initiated by zinc ion, which promotes the ionization of bound H2O and results in nucleophilic attack on CO2 to generate hydrated carbon dioxide (bicarbonate, HCO3–). The second step is the transfer of a proton from bulk solvent to the zinc-bound hydroxide ion, regenerating the zinc-bound water and resetting the enzyme. In this process, H64 (in human α-CA II) acts as a proton shuttle via a side chain movement from “in” (pointing toward the active site) to “out” (pointing toward the solvent) (Fisher et al., 2007; Maupin and Voth, 2007; West et al., 2012). In the past decade, highly thermo- and alkali-stable α-CA enzymes from mycobacteria and algae have attracted great attention for mitigating global warming because biomineralization of CO2 using these enzymes is one of the most economical ways of permanently storing carbon (Capasso et al., 2012b; Jo et al., 2014; Luca et al., 2013; Russo et al., 2013).
β-CAs were first discovered in plant leaf chloroplasts, but have since been found in bacteria, fungi, and algae (Neish, 1939; Smith et al., 1999). Based on the amino acid sequence and structure, they are divided into two main subgroups: plant-type tetramers such as that in
The functions of four β-CAs (CafA-D) also have been determined in
Recently, we solved the crystal structures of CafC and CafD by X-ray crystallography (Kim et al., 2019a). Using these structures, combined with our biochemical data, we revealed the molecular basis underlying their low activities. In CafC, access of CO2 to the active site is limited by a narrow opening. Meanwhile, CafD exists predominantly in an inactive form because a conformational change of an aspartic acid induced by formation of an Asp-Arg pair acts as an on/off switch, which is inhibited by substitution of a glycine for an arginine.
In the present study, the crystal structures of CafA in the absence and presence of the potent inhibitor acetazolamide were determined at 1.8 Å and 2.0 Å resolution, respectively. Together with our biochemical results, our findings establish the molecular basis for understanding the catalytic mechanism of the major β-CAs in
A codon-optimized gene encoding CafA (residues 75-287) of
After harvesting cells and lysing using a microfluidizer, CafA protein was purified using glutathione agarose beads (GoldBio, USA). After thorough washing, the protein was eluted by on-column thrombin cleavage in buffer containing 20 mM TRIS-HCl pH 8.0 and 200 mM NaCl. The protein was further purified by HiTrap Q anion exchange chromatography (GE Healthcare, USA) and Superdex 200 gel filtration chromatography (GE Healthcare). All purification steps were performed on ice or at 4°C.
To measure thermal stability, proteins were pretreated at temperatures from 22°C to 100°C for 15 min. To measure pH stability, 20 µl protein was incubated at 4°C for 4 h in 80 µl of 50 mM buffer at different pH values between 4 and 10. Remaining enzymatic activity was measured by
Purified CafA was concentrated to 15-20 mg/ml for crystallization. Crystals were obtained at 22°C by the sitting-drop vapor diffusion method in 0.1 M MES (or TRIS-HCl) pH 6.0-7.5, 24%-30% (w/v) polyethylene glycol 2000 monomethyl ether (PEG2000MME), and 0.2-0.4 M sodium acetate. For co-crystallization, acetazolamide (Sigma-Aldrich) was added to a concentrated solution of protein at a 10:1 molar ratio and incubated on ice for 30 min. Crystals of CafA were cryoprotected in reservoir solution supplemented with ethylene glycerol and then flash-frozen in liquid nitrogen. X-ray diffraction data were collected at beamline 7A of the Pohang Accelerator Laboratory (PAL, Korea). Datasets were indexed, integrated, and scaled using the HKL2000 program (Otwinowski and Minor, 1997). The CafA structure was determined by molecular replacement using the structure of β-CA CAS2 from the fungus
Coordinates and structure factors for CafA have been deposited in the PDB under accession codes 7COI (apo, crystal form 1) and 7COJ (acetazolamide-bound, crystal form 2).
To investigate the oligomeric state of CafA in solution, the protein was expressed in bacteria and analyzed by size exclusion chromatography. Originally, we expressed full-length CafA, but it was expressed as two bands with similar densities as judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (Fig. 1A). CafA was predicted to be a mitochondrial protein processed to its mature form by cleavage of its N-terminal mitochondrial targeting sequence (Han et al., 2010); hence we thought that overexpression might reduce the efficiency of this process. Therefore, using the MitoFates program (Fukasawa et al., 2015), we constructed two truncated variants of CafA in which the mitochondrial targeting peptide was eliminated to achieve a homogenous product. Based on expression level, a construct comprising CafA residues 75-287 was chosen for further biochemical and crystallographic studies (Fig. 1A). The final CafA construct used in our experiments comprised 213 amino acids with a predicted molecular mass of 23 kDa. Analysis by size exclusion chromatography showed that CafA eluted as a single peak at a position corresponding to the expected molecular mass for a tetramer (92 kDa; Fig. 1B). The fact that the tetramers persisted during gel filtration chromatography suggests that their tetrameric configurations are stabilized by strong interfacial interactions.
To determine the catalytic activities of the purified enzyme,
Next, we explored the thermal and pH stability of CafA. As shown in Fig. 2A, CafA retained > 70% of its initial activity after incubation at temperatures up to 40°C, although it retained only 30% activity after 50°C treatment, and activity was almost negligible after exposure to temperatures above 50°C. To test the effect of pH on enzyme activity, proteins were pre-incubated in solutions with different pH values (pH 4-10). CafA was stable over a wide pH range (pH 5-9), but was rapidly inactivated below pH 5 or above pH 10 (Fig. 2B). This is reminiscent of previous studies showing that
Acetazolamide is a potent sulfonamide-based CA inhibitor. Its inhibition of enzymatic activity of CAs has been studied extensively as CAs are essential for the survival of many cancer cells under acidic and hypoxic stress (Neri and Supuran, 2011). Recently anions were also shown to inhibit the catalytic activity of fungal β-CAs (Innocenti et al., 2008; 2009; Isik et al., 2008). To determine the effect of anions on the activities of CafA, various concentrations of nitrate (NO3–), nitrite (NO2–), and sulfate (SO42–) ions were separately added, and mixtures were incubated on ice for 30 min before measuring CO2 hydration. Acetazolamide was used as a positive control. As expected, CafA activity was reduced by increasing the acetazolamide concentration and was completely inhibited at 5 µM acetazolamide (Fig. 2C). Interestingly, CafA was also weakly inhibited by nitrate in the submillimolar range (Fig. 2D). By contrast, nitrogen dioxide and sulfate had no inhibitory effect over the concentration ranges tested. This result suggests that nitrate or nitrate-containing compounds may represent a promising class of antifungal agents for the treatment of invasive aspergillosis (Supuran, 2008).
To establish the structural basis for the catalytic activity of CafA, we crystallized the purified protein in the presence and absence of acetazolamide, and determined the structures by X-ray crystallography (Table 1). Residues of the N-terminus (75-76), the linker region (97-101 and 269-275), and the C-terminus (286-287) could not be fitted effectively into the electron density map and were therefore not included in the final model. In our structure, CafA forms a plant-type tetramer from a dimer of dimers in the asymmetric unit, consistent with the gel filtration data (Figs. 1B and 3A). We observed that tetramerization is driven by the association of pairs of relatively flat surfaces composed of α-helices orthogonal to the dimerization interfaces. CafA possess the typical structural features of other plant-type β-CAs (Figs. 3B and 4) (Cronk et al., 2006; Dostál et al., 2018; Lehneck et al., 2014; Schlicker et al., 2009). The overall structure of each protomer consists of three parts: an N-terminal α-helical extension, a conserved central core comprising 10 α-helices and 5 β-strands, and a C-terminal subdomain (Fig. 3B). The N-terminal α-helix, which includes two perpendicularly oriented helices, extends far out from the central core region, spanning the neighboring protomer and stabilizing dimer interface interactions. The core structures are composed of four parallel β-strands (β2-β1-β3-β4) accompanied by a fifth antiparallel β-strand (β5). The C-terminal subdomains flank the adjacent protomers, creating new contacts, thus further stabilizing tetramer formation. Structure-based sequence alignment showed that CafA shares a high degree of amino acid conservation with CafB, suggesting that they might share a similar protein fold and catalytic mechanism (Fig. 5). Note that throughout this article, single apostrophes are used to differentiate residues from the other protomer of the dimer.
CafA (form 1) has a typical Type-I or open conformation in which the substrate binds at the dimer interface (Fig. 6). The catalytic zinc ion is tetrahedrally coordinated by three conserved residues (C119, H175, C178) and an acetate anion presumably acquired from the crystallization solution that replaces zinc-bound water in the metal coordination sphere (Fig. 6). The carboxyl group of acetate is firmly positioned by strong hydrogen bonds with the side chains of D121 and Q110’ and the backbone nitrogen of G179. By contrast, the methyl group of acetate is oriented toward the hydrophobic side of the dimer interface composed of I143, F138’, and F160’. The highly conserved D121 also forms essential salt bridges with R123 (the Asp-Arg pair).
We also solved the crystal structure of CafA in complex with acetazolamide (form 2) (Fig. 7A). The binding mode of acetazolamide is similar to that reported previously for β-CAs (Huang et al., 2011). The sulfonamide group of acetazolamide is directly bound to the zinc ion, replacing the native water molecule in the zinc coordination sphere, indicating that this conformation is catalytically inactive (Figs. 7B and 7C). The amide and hydroxyl groups of the sulfonamide motif form hydrogen bonds with the side chains of D121 and Q110’, respectively, while the nitrogen atom of the acetylamide group interacts with the backbone oxygen of G179. The thiadiazole ring is sandwiched between G179 and F160’. Comparison of the CafA structures with and without acetazolamide reveals minor differences in this sandwich region; in the presence of the inhibitor, the phenyl ring of F160’ undergoes a ~40° rotation, and G179 moves ~0.6 Å away from the thiadiazole ring to avoid steric clashes with the bound acetazolamide (Fig. 7D). It is noteworthy that no local conformational changes are induced in α-CAs by binding of acetazolamide due to their large active sites, which are fully exposed to the solvent (Eriksson et al., 1988; James et al., 2014; Nair et al., 1995).
In summary, the structure of CafA reveals key features that may be responsible for its apparent
We thank the staff at beamline 7A of PAL, and Dr. Julian Gross for critical reading of the manuscript. This work was supported by grants from the National Research Foundation (NRF), funded by the Ministry of Science, ICT, and Future Planning of Korea (grant numbers NRF-2017M3A9F6029753 and NRF-2019M3E5D6063908) and from the GIST Research Institute (GRI) IIBR, funded by the GIST in 2020.
All authors contributed to the study design, performed experiments, and interpreted the data. S.K., J.Y., and J.S. purified and crystallized the protein. S.K. and J.Y. performed enzyme activity assays. S.K. determined the crystal structures. S.K. and J.Y. prepared the figures. S.K. and M.S.J. wrote the manuscript. All authors helped to improve the manuscript and approved the final version.
The authors have no potential conflicts of interest to disclose.
Mol. Cells 2020; 43(9): 831-840
Published online September 30, 2020 https://doi.org/10.14348/molcells.2020.0168
Copyright © The Korean Society for Molecular and Cellular Biology.
Subin Kim , Jungyoon Yeon
, Jongmin Sung
, and Mi Sun Jin
*
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea
Correspondence to:*misunjin@gist.ac.kr
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
The β-class of carbonic anhydrases (β-CAs) are zinc metalloenzymes widely distributed in the fungal kingdom that play essential roles in growth, survival, differentiation, and virulence by catalyzing the reversible interconversion of carbon dioxide (CO2) and bicarbonate (HCO3–). Herein, we report the biochemical and crystallographic characterization of the β-CA CafA from the fungal pathogen Aspergillus fumigatus, the main causative agent of invasive aspergillosis. CafA exhibited apparent in vitro CO2 hydration activity in neutral to weak alkaline conditions, but little activity at acidic pH. The high-resolution crystal structure of CafA revealed a tetramer comprising a dimer of dimers, in which the catalytic zinc ion is tetrahedrally coordinated by three conserved residues (C119, H175, C178) and an acetate anion presumably acquired from the crystallization solution, indicating a freely accessible ″open″ conformation. Furthermore, knowledge of the structure of CafA in complex with the potent inhibitor acetazolamide, together with its functional intolerance of nitrate (NO3–) ions, could be exploited to develop new antifungal agents for the treatment of invasive aspergillosis.
Keywords: β-class carbonic anhydrase, Aspergillus fumigatus, CafA, X-ray crystallography, zinc metalloenzyme
Carbonic anhydrases (CAs) are widely distributed zinc metalloenzymes that catalyze the interconversion of carbon dioxide to bicarbonate and a proton (Supuran, 2016). They are currently divided into seven families (α, β, γ, δ, ζ, η, θ), which are evolutionarily unrelated in amino acid sequence and structure (Del Prete et al., 2014a; 2014b; Iverson et al., 2000; Kikutani et al., 2016; Meldrum and Roughton, 1933; Mitsuhashi et al., 2000; Xu et al., 2008). Of the different classes, α- and β-CAs have been well-characterized biophysically and structurally. α-CAs are found in mammals, fungi, prokaryotes, and plants. All known bacterial α-CAs are dimers (Kim et al., 2019b; Kimber and Pai, 2000), while most mammalian CAs are monomers, with some also possessing dimeric α-CA forms (Alterio et al., 2009; Pilka et al., 2012; Whittington et al., 2001). α-CAs have a catalytic zinc ion coordinated by three conserved histidine residues and a water molecule (or hydroxide ion) in their active sites (Nair et al., 1995). Extensive structural and biochemical analysis has led to the two-step CO2 hydration model for the α-CA family (Silverman and Lindskog, 1988). In this model, the first step is initiated by zinc ion, which promotes the ionization of bound H2O and results in nucleophilic attack on CO2 to generate hydrated carbon dioxide (bicarbonate, HCO3–). The second step is the transfer of a proton from bulk solvent to the zinc-bound hydroxide ion, regenerating the zinc-bound water and resetting the enzyme. In this process, H64 (in human α-CA II) acts as a proton shuttle via a side chain movement from “in” (pointing toward the active site) to “out” (pointing toward the solvent) (Fisher et al., 2007; Maupin and Voth, 2007; West et al., 2012). In the past decade, highly thermo- and alkali-stable α-CA enzymes from mycobacteria and algae have attracted great attention for mitigating global warming because biomineralization of CO2 using these enzymes is one of the most economical ways of permanently storing carbon (Capasso et al., 2012b; Jo et al., 2014; Luca et al., 2013; Russo et al., 2013).
β-CAs were first discovered in plant leaf chloroplasts, but have since been found in bacteria, fungi, and algae (Neish, 1939; Smith et al., 1999). Based on the amino acid sequence and structure, they are divided into two main subgroups: plant-type tetramers such as that in
The functions of four β-CAs (CafA-D) also have been determined in
Recently, we solved the crystal structures of CafC and CafD by X-ray crystallography (Kim et al., 2019a). Using these structures, combined with our biochemical data, we revealed the molecular basis underlying their low activities. In CafC, access of CO2 to the active site is limited by a narrow opening. Meanwhile, CafD exists predominantly in an inactive form because a conformational change of an aspartic acid induced by formation of an Asp-Arg pair acts as an on/off switch, which is inhibited by substitution of a glycine for an arginine.
In the present study, the crystal structures of CafA in the absence and presence of the potent inhibitor acetazolamide were determined at 1.8 Å and 2.0 Å resolution, respectively. Together with our biochemical results, our findings establish the molecular basis for understanding the catalytic mechanism of the major β-CAs in
A codon-optimized gene encoding CafA (residues 75-287) of
After harvesting cells and lysing using a microfluidizer, CafA protein was purified using glutathione agarose beads (GoldBio, USA). After thorough washing, the protein was eluted by on-column thrombin cleavage in buffer containing 20 mM TRIS-HCl pH 8.0 and 200 mM NaCl. The protein was further purified by HiTrap Q anion exchange chromatography (GE Healthcare, USA) and Superdex 200 gel filtration chromatography (GE Healthcare). All purification steps were performed on ice or at 4°C.
To measure thermal stability, proteins were pretreated at temperatures from 22°C to 100°C for 15 min. To measure pH stability, 20 µl protein was incubated at 4°C for 4 h in 80 µl of 50 mM buffer at different pH values between 4 and 10. Remaining enzymatic activity was measured by
Purified CafA was concentrated to 15-20 mg/ml for crystallization. Crystals were obtained at 22°C by the sitting-drop vapor diffusion method in 0.1 M MES (or TRIS-HCl) pH 6.0-7.5, 24%-30% (w/v) polyethylene glycol 2000 monomethyl ether (PEG2000MME), and 0.2-0.4 M sodium acetate. For co-crystallization, acetazolamide (Sigma-Aldrich) was added to a concentrated solution of protein at a 10:1 molar ratio and incubated on ice for 30 min. Crystals of CafA were cryoprotected in reservoir solution supplemented with ethylene glycerol and then flash-frozen in liquid nitrogen. X-ray diffraction data were collected at beamline 7A of the Pohang Accelerator Laboratory (PAL, Korea). Datasets were indexed, integrated, and scaled using the HKL2000 program (Otwinowski and Minor, 1997). The CafA structure was determined by molecular replacement using the structure of β-CA CAS2 from the fungus
Coordinates and structure factors for CafA have been deposited in the PDB under accession codes 7COI (apo, crystal form 1) and 7COJ (acetazolamide-bound, crystal form 2).
To investigate the oligomeric state of CafA in solution, the protein was expressed in bacteria and analyzed by size exclusion chromatography. Originally, we expressed full-length CafA, but it was expressed as two bands with similar densities as judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (Fig. 1A). CafA was predicted to be a mitochondrial protein processed to its mature form by cleavage of its N-terminal mitochondrial targeting sequence (Han et al., 2010); hence we thought that overexpression might reduce the efficiency of this process. Therefore, using the MitoFates program (Fukasawa et al., 2015), we constructed two truncated variants of CafA in which the mitochondrial targeting peptide was eliminated to achieve a homogenous product. Based on expression level, a construct comprising CafA residues 75-287 was chosen for further biochemical and crystallographic studies (Fig. 1A). The final CafA construct used in our experiments comprised 213 amino acids with a predicted molecular mass of 23 kDa. Analysis by size exclusion chromatography showed that CafA eluted as a single peak at a position corresponding to the expected molecular mass for a tetramer (92 kDa; Fig. 1B). The fact that the tetramers persisted during gel filtration chromatography suggests that their tetrameric configurations are stabilized by strong interfacial interactions.
To determine the catalytic activities of the purified enzyme,
Next, we explored the thermal and pH stability of CafA. As shown in Fig. 2A, CafA retained > 70% of its initial activity after incubation at temperatures up to 40°C, although it retained only 30% activity after 50°C treatment, and activity was almost negligible after exposure to temperatures above 50°C. To test the effect of pH on enzyme activity, proteins were pre-incubated in solutions with different pH values (pH 4-10). CafA was stable over a wide pH range (pH 5-9), but was rapidly inactivated below pH 5 or above pH 10 (Fig. 2B). This is reminiscent of previous studies showing that
Acetazolamide is a potent sulfonamide-based CA inhibitor. Its inhibition of enzymatic activity of CAs has been studied extensively as CAs are essential for the survival of many cancer cells under acidic and hypoxic stress (Neri and Supuran, 2011). Recently anions were also shown to inhibit the catalytic activity of fungal β-CAs (Innocenti et al., 2008; 2009; Isik et al., 2008). To determine the effect of anions on the activities of CafA, various concentrations of nitrate (NO3–), nitrite (NO2–), and sulfate (SO42–) ions were separately added, and mixtures were incubated on ice for 30 min before measuring CO2 hydration. Acetazolamide was used as a positive control. As expected, CafA activity was reduced by increasing the acetazolamide concentration and was completely inhibited at 5 µM acetazolamide (Fig. 2C). Interestingly, CafA was also weakly inhibited by nitrate in the submillimolar range (Fig. 2D). By contrast, nitrogen dioxide and sulfate had no inhibitory effect over the concentration ranges tested. This result suggests that nitrate or nitrate-containing compounds may represent a promising class of antifungal agents for the treatment of invasive aspergillosis (Supuran, 2008).
To establish the structural basis for the catalytic activity of CafA, we crystallized the purified protein in the presence and absence of acetazolamide, and determined the structures by X-ray crystallography (Table 1). Residues of the N-terminus (75-76), the linker region (97-101 and 269-275), and the C-terminus (286-287) could not be fitted effectively into the electron density map and were therefore not included in the final model. In our structure, CafA forms a plant-type tetramer from a dimer of dimers in the asymmetric unit, consistent with the gel filtration data (Figs. 1B and 3A). We observed that tetramerization is driven by the association of pairs of relatively flat surfaces composed of α-helices orthogonal to the dimerization interfaces. CafA possess the typical structural features of other plant-type β-CAs (Figs. 3B and 4) (Cronk et al., 2006; Dostál et al., 2018; Lehneck et al., 2014; Schlicker et al., 2009). The overall structure of each protomer consists of three parts: an N-terminal α-helical extension, a conserved central core comprising 10 α-helices and 5 β-strands, and a C-terminal subdomain (Fig. 3B). The N-terminal α-helix, which includes two perpendicularly oriented helices, extends far out from the central core region, spanning the neighboring protomer and stabilizing dimer interface interactions. The core structures are composed of four parallel β-strands (β2-β1-β3-β4) accompanied by a fifth antiparallel β-strand (β5). The C-terminal subdomains flank the adjacent protomers, creating new contacts, thus further stabilizing tetramer formation. Structure-based sequence alignment showed that CafA shares a high degree of amino acid conservation with CafB, suggesting that they might share a similar protein fold and catalytic mechanism (Fig. 5). Note that throughout this article, single apostrophes are used to differentiate residues from the other protomer of the dimer.
CafA (form 1) has a typical Type-I or open conformation in which the substrate binds at the dimer interface (Fig. 6). The catalytic zinc ion is tetrahedrally coordinated by three conserved residues (C119, H175, C178) and an acetate anion presumably acquired from the crystallization solution that replaces zinc-bound water in the metal coordination sphere (Fig. 6). The carboxyl group of acetate is firmly positioned by strong hydrogen bonds with the side chains of D121 and Q110’ and the backbone nitrogen of G179. By contrast, the methyl group of acetate is oriented toward the hydrophobic side of the dimer interface composed of I143, F138’, and F160’. The highly conserved D121 also forms essential salt bridges with R123 (the Asp-Arg pair).
We also solved the crystal structure of CafA in complex with acetazolamide (form 2) (Fig. 7A). The binding mode of acetazolamide is similar to that reported previously for β-CAs (Huang et al., 2011). The sulfonamide group of acetazolamide is directly bound to the zinc ion, replacing the native water molecule in the zinc coordination sphere, indicating that this conformation is catalytically inactive (Figs. 7B and 7C). The amide and hydroxyl groups of the sulfonamide motif form hydrogen bonds with the side chains of D121 and Q110’, respectively, while the nitrogen atom of the acetylamide group interacts with the backbone oxygen of G179. The thiadiazole ring is sandwiched between G179 and F160’. Comparison of the CafA structures with and without acetazolamide reveals minor differences in this sandwich region; in the presence of the inhibitor, the phenyl ring of F160’ undergoes a ~40° rotation, and G179 moves ~0.6 Å away from the thiadiazole ring to avoid steric clashes with the bound acetazolamide (Fig. 7D). It is noteworthy that no local conformational changes are induced in α-CAs by binding of acetazolamide due to their large active sites, which are fully exposed to the solvent (Eriksson et al., 1988; James et al., 2014; Nair et al., 1995).
In summary, the structure of CafA reveals key features that may be responsible for its apparent
We thank the staff at beamline 7A of PAL, and Dr. Julian Gross for critical reading of the manuscript. This work was supported by grants from the National Research Foundation (NRF), funded by the Ministry of Science, ICT, and Future Planning of Korea (grant numbers NRF-2017M3A9F6029753 and NRF-2019M3E5D6063908) and from the GIST Research Institute (GRI) IIBR, funded by the GIST in 2020.
All authors contributed to the study design, performed experiments, and interpreted the data. S.K., J.Y., and J.S. purified and crystallized the protein. S.K. and J.Y. performed enzyme activity assays. S.K. determined the crystal structures. S.K. and J.Y. prepared the figures. S.K. and M.S.J. wrote the manuscript. All authors helped to improve the manuscript and approved the final version.
The authors have no potential conflicts of interest to disclose.
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