Mol. Cells 2020; 43(9): 774-783
Published online August 14, 2020
https://doi.org/10.14348/molcells.2020.0059
© The Korean Society for Molecular and Cellular Biology
Correspondence to : *jkyoon@sch.ac.kr
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
The lung has a vital function in gas exchange between the blood and the external atmosphere. It also has a critical role in the immune defense against external pathogens and environmental factors. While the lung is classified as a relatively quiescent organ with little homeostatic turnover, it shows robust regenerative capacity in response to injury, mediated by the resident stem/progenitor cells. During regeneration, regionally distinct epithelial cell populations with specific functions are generated from several different types of stem/progenitor cells localized within four histologically distinguished regions: trachea, bronchi, bronchioles, and alveoli. WNT signaling is one of the key signaling pathways involved in regulating many types of stem/progenitor cells in various organs. In addition to its developmental role in the embryonic and fetal lung, WNT signaling is critical for lung homeostasis and regeneration. In this minireview, we summarize and discuss recent advances in the understanding of the role of WNT signaling in lung regeneration with an emphasis on stem/progenitor cells.
Keywords β-catenin, lung homeostasis, lung regeneration, lung stem/progenitor cells, WNT signaling
The lung is a structurally complex organ comprising at least 40 different types of cells with diverse functions. It can be sub-divided anatomically into four different regions—trachea, bronchi, bronchioles, and alveoli. To fulfill its different functions, the cellular composition and three-dimensional structure of the lung must be maintained throughout an organism’s lifetime. Under normal conditions, the lung shows a low rate of cellular turnover relative to other organs, such as the skin and intestine, which exhibit rapid kinetics of cellular replacement characteristics (Bowden et al., 1968; Kauffman, 1980; Wansleeben et al., 2013). However, after injury or damage caused by different agents, including infection, toxic compounds, and irradiation, the lung demonstrates a remarkable ability to regenerate the damaged tissue (Hogan et al., 2014; Lee and Rawlins, 2018; Wansleeben et al., 2013). If this regeneration process is not completed successfully, it leads to a disruption in proper lung function accompanied by chronic inflammation, pathologic remodeling, and fibrosis.
Normal cell turnover and regeneration after injury in the lung require regionally- specific epithelial stem/progenitor cells and unique microenvironment or neighboring cells that can crosstalk with each other (Lee and Rawlins, 2018; Rock and Hogan, 2011; Wansleeben et al., 2013). It has been challenging to distinguish the specific properties of lung stem/progenitor cells owing to the histological complexity of the lung and the multiplicity of cell types, besides the slow turnover of the respiratory epithelium. Each region comprises different cell types and the homeostasis in each region is guaranteed by regionally-specific stem/progenitor cells (Ardhanareeswaran and Mirotsou, 2013; Spurlin and Nelson, 2017; Wansleeben et al., 2013). In addition, the regenerative capacity of these lung stem/progenitor cells is not only determined by their intrinsic potential, but also by the microenvironment of their specific niche, which includes the extracellular matrix, stromal and other types of cells, as well as, the many signaling factors derived from them (Hogan et al., 2014; Lee and Rawlins, 2018; Stabler and Morrisey, 2017).
The WNT signaling pathway plays major roles during embryogenesis, tissue homeostasis, and regeneration in many organs (Kahn, 2018; Nusse and Clevers, 2017; Steinhart and Angers, 2018). The functional importance of WNT signaling in the lung is initially demonstrated during lung development, as early as at the stage of lung endoderm specification (Hussain et al., 2017; Majidinia et al., 2018). Recent studies have begun to reveal the roles of WNT signaling in lung homeostasis and regeneration. In this review, we discuss the recent findings on the role of WNT signaling in lung homeostasis and regeneration, while highlighting different lung stem/progenitor cells and the mechanisms that regulate their behavior and function.
Secreted WNT ligands, comprising 19 members, are highly conserved signaling molecules that activate downstream signaling cascades involved in the regulation of gene expression, proliferation, differentiation, adhesion and migration, and polarity in signaling-receiving cells (Kahn, 2018; Nusse and Clevers, 2017; Steinhart and Angers, 2018). The two major WNT signaling pathways are canonical (WNT/β-catenin) and non-canonical pathways (Fig. 1). WNT1 (WNT2, WNT3, WNT3A, and WNT8A) and WNT5A (WNT4, WNT5A, WNT5B, WNT6, WNT7A, and WNT11) class ligands primarily determine the specificity of activation of the canonical and non-canonical pathways, respectively. In the canonical WNT signaling, binding of the WNT1 class ligands to the seven-pass trans-membrane Frizzled receptor family (FZD) and the low-density lipoprotein receptor-related protein family (LRP5/6) initiates a signaling cascade. A hallmark of this pathway is an accumulation of the normally unstable β-catenin protein within the cytoplasm through the inhibition of glycogen synthase kinase 3 beta (GSK-3β) and the subsequent translocation of β-catenin to the nucleus, where it engages in gene transcription (Nusse and Clevers, 2017; Steinhart and Angers, 2018). In contrast, non-canonical WNT signaling is activated by WNT5A class ligands via the FZD receptors, independent of LRP5/6 receptors (Chae and Bothwell, 2018; Steinhart and Angers, 2018). This ligand-receptor binding initiates the activation of WNT/PCP (planar cell polarity) and WNT/Ca2+ pathways. The WNT/PCP pathway activates JNK and Rho-kinase cascades, whereas the WNT/Ca2+ pathway increases intracellular calcium concentration and activates protein kinase C (PKC), calcineurin, and calcium/calmodulin-dependent protein kinase II cascades. In addition to the typical FZD and LRP5/6 receptors, receptor tyrosine kinases (RYK) and retinoic acid-related orphan receptors are also known to function in a cell type-specific manner as cognate receptors for canonical and non-canonical WNT signaling, respectively (Chae and Bothwell, 2018; Nusse and Clevers, 2017). Furthermore, while WNT signaling is positively regulated through secretory agonists, such as R-spondins (RSPOs) and norrin, it is negatively regulated through secretory antagonists, such as dickkopf-1 (DKK1), secreted FZD-related proteins, WNT inhibitory factor 1, and sclerostin (Chae and Bothwell, 2018; Nusse and Clevers, 2017; Raslan and Yoon, 2019).
The epithelial cells of the airway walls represent an effective physical barrier against the external atmosphere and are the first cell type that are damaged by exogenous microorganisms, airborne irritants, and allergens. The epithelial cells exhibit unique stereotypic patterns from the proximal airway axis to the distal airway axis. Multiple studies have shown that diverse types of cells, possessing stem/progenitor-like behavior in response to airway injury, are present throughout the airways (Lee and Rawlins, 2018; Rock et al., 2010; Stabler and Morrisey, 2017; Wansleeben et al., 2013). Both canonical WNT/β-catenin and non-canonical WNT signaling appear to play diverse roles in various stem/progenitor cells and niches cells in a region-dependent manner during regeneration.
A pseudo-stratified columnar epithelium, consisting of basal, secretory (goblet and club [or Clara]), and ciliated cells, lines the trachea and bronchi (Fig. 2) (Hogan et al., 2014; Mercer et al., 1994; Wansleeben et al., 2013). In humans, a majority of the epithelial cells in the trachea are ciliated (Hogan and Tata, 2019; Meyerholz et al., 2018). In contrast, the majority of the epithelial cells in mouse trachea are non-ciliated (Hogan and Tata, 2019; Meyerholz et al., 2018). Moreover, few goblet cells and more club cells exist in mice trachea than in humans (Hogan and Tata, 2019; Meyerholz et al., 2018). Bronchioles contain simple columnar epithelial cells, including club, ciliated, and pulmonary neuroendocrine cells (NECs). NECs are more solitary and scattered in the airways in humans than in mice (Hogan and Tata, 2019; Meyerholz et al., 2018).
Two tracheal cell populations have been identified as stem/progenitor cells in response to injury. The first population is that of basal cells (BCs) that comprise about 30% of the total epithelial population in the trachea. BCs are pluripotent and express the transcription factor TRP63, cytokeratin 5 (KRT5), and the nerve growth factor receptor (NGFR) (Fig. 3, Table 1) (Hogan et al., 2014; Rock et al., 2009; Tata and Rajagopal, 2017). In steady-state and regeneration following damage, BCs can self-renew and differentiate into multiple cell lineages, including ciliated and secretory cells
Independent of BCs, tracheal club cells expressing secretoglobin 1A1 (SCGB1A1, also known as CCSP, CC10, or uteroglobin) display a limited stem/progenitor cell property (Fig. 3, Table 1). While tracheal club cells exhibit a relatively slow rate of self-renewal (Rawlins et al., 2009), upon tracheal injury, they are able to give rise to ciliated cells, but not to other types of cells
In the trachea of adult mice, canonical WNT signaling is activated within BCs following tracheal damage (Brechbuhl et al., 2011; Giangreco et al., 2012) (Fig. 4). In a transgenic mouse model with dominant-negative (N-terminally truncated) lymphoid enhancer-binding factor1 (LEF1) expression driven under the
Unlike trachea, the BC population decreases significantly in the main stem bronchi and is absent in the columnar epithelium of the distal bronchioles in the mouse lung. In contrast, BCs are present throughout all bronchi and bronchioles in the human lung. The goblet cells are very rare in mouse bronchioles airways (Hogan and Tata, 2019; Meyerholz et al., 2018). The epithelia of distal bronchioles comprise club, multi-ciliate, and NECs (Fig. 2) (Hogan and Tata, 2019; Meyerholz et al., 2018). Following naphthalene-induced injury
In the most distant region of the airways, bronchioalveolar stem cells (BASCs) that co-express SCGB1A1 (a club cell marker) and surfactant protein C, SFTPC (an AT2 cell marker) are identified in the bronchioalveolar duct junction. These cells are distinct from UPK3A+ club cells (Fig. 2) (Kim et al., 2005; Salwig et al., 2019). In response to airway injury by naphthalene or influenza virus infection, these BASCs yield diverse populations of differentiated airway cells, including club and ciliated cells
After naphthalene-induced lung injury, WNT ligands (WMT3A, WNT5A, and WNT7B) are upregulated in club cells at an early stage of regeneration (Lee et al., 2017). This indicates the importance of WNT signaling in the regeneration of the bronchiolar epithelium. Surprisingly, club cell-specific deletion of the
Several studies provide additional clues regarding the role of WNT signaling in bronchiole regeneration. Expression of leucine-rich repeat containing G protein-coupled receptor 6 (LGR6), a specific receptor for the WNT positive regulator, RSPOs, and a known marker for many types of stem cells (Barker et al., 2013; Raslan and Yoon, 2019; Schindler et al., 2018) is detected in airway smooth muscle cells (ASMCs), a stromal niche of club cells surrounding the airway epithelia (Lee et al., 2017). These LGR6+ ASMCs play an important role in the regeneration of the airway epithelia. Genetic depletion of LGR6+ ASMCs causes significant defects in airway repair following naphthalene-induced injury in the lung
According to a recent report, club cell-specific deletion of the gene for the canonical WNT receptor,
Alveoli are populated by squamous, gas exchanging AT1 cells and cuboidal, surfactant secreting AT2 cells (Fig. 2). ATI cells are thin, flat cells that cover 95% of the alveolar surface (Fig. 2). They can be identified by the expression of markers such as aquaporin 5 (AQP5), podoplanin (PDPN), RAGE, and HOPX. It has long been considered that AT1 is the most vulnerable target for distal lung injury and aging. AT2 cells express markers such as SFTPC and a lipid transporter, ATP binding cassette A3 transporter (Fig. 3, Table 1). Similar to other epithelial cells in the lung, the alveolar epithelial cells also show a relatively slow rate of cellular turnover (about 28-35 days) under normal conditions. However, after injury by agents such as hyperoxia, NO2, ozone, and bleomycin, alveoli show robust regeneration capacity through extensive cell proliferation and differentiation (Barkauskas et al., 2013; Desai et al., 2014; Lee and Rawlins, 2018; Olajuyin et al., 2019).
Several distinct cell populations are identified as alveolar stem/progenitor cells. Firstly, in the alveolar-injury model induced by bleomycin or influenza virus infection, BASCs localized within the bronchioalveolar region expand and differentiate into AT1 and AT2 cells
Within the alveoli of the normal adult lung, most epithelial cells, including AT2 cells, do not exhibit active canonical WNT signaling. Canonical WNT signaling within AT2 cells gets activated only after bleomycin-induced injury (Flozak et al., 2010; Tanjore et al., 2013). Furthermore, in the bleomycin-injured alveoli, an increase in the number of β-catenin-positive nuclei, as well as, the expression of canonical
In addition to the role in AT2 cell proliferation and survival, both canonical and non-canonical WNT signaling pathways have been shown to regulate AT2 cell differentiation. It is known that the interaction between p300 co-activator and β-catenin increases during the differentiation of AT2 cells into AT1 cells (Rieger et al., 2016). While a nuclear accumulation of β-catenin is induced by canonical WNT signaling, the regulation of p300/β-catenin interaction occurs through the non-canonical WNT5A/PKC signaling axis (Rieger et al., 2016). WNT5A regulates Ser-89 phosphorylation of p300 in a PKC-dependent manner, resulting in enhanced interaction with β-catenin. Inhibition of p300/β-catenin interaction by IQ-1, a specific small-molecule inhibitor, impairs the differentiation of primary AT2 cells. Unlike this study, Frank et al. (2016) showed that canonical WNT signaling inhibits AT2 cell differentiation. It is not yet defined why these contrasting results exist. Nonetheless, both canonical WNT3A and non-canonical WNT5A ligands are derived from the neighboring LGR5+ stromal cells to promote alveolar differentiation of both club and AT2 cells (Lee et al., 2017). Moreover, resident alveolar macrophages also produce WNT ligands, especially WNT4 and WNT16, to stimulate AT2 cell proliferation and improve alveoli regeneration after lung injury induced by
A recent report showed that non-canonical WNT5A and WNT5B ligands repress the proliferation of alveolar epithelial stem/progenitor cells (Wu et al., 2019). It has been demonstrated in
In addition to the alveolar stem/progenitor cells, WNT signaling also plays a critical role in WNT-producing stromal cells, especially the AXIN2+ PDGFRα+ mesenchymal alveolar niche cells during regeneration (Basil et al., 2020; Zepp et al., 2017). These cells are demonstrated to promote the self-renewal and differentiation of AT2 cells during alveoli homeostasis and regeneration by providing different signals such as IL6, FGFs, and BMP antagonists likely induced by canonical WNT signaling. It still remains to be determined whether WNT-producing LGR5+ stromal cells and mesenchymal alveolar niche cells are the same population or not. Given that both
The robust regenerative capacity of the lung after injury reflects the histological complexity of the lung. As the different regions of the lung contain unique populations of stem/progenitor cells and their microenvironment, an efficient and correct regeneration process appears to be imprinted within the identities and differentiation potentials of stem cells and their niche. Both canonical and non-canonical WNT signaling pathways play critical regulatory roles in the function and behavior of the different lung stem cell populations and their niche cells. Especially after lung injury, activated canonical WNT signaling is crucial for proliferation, survival, and differentiation of lung epithelial stem/progenitor cells. In contrast, the functional roles of non-canonical WNT signaling during regeneration are relatively limited and need to be further explored in the future.
Disruption of proper WNT signaling during regeneration results in inefficient regeneration. This insufficient repair and repetitive injury promote impairment of the epithelial-mesenchymal crosstalk for lung homeostasis, which plays a key role in the pathogenesis of chronic lung diseases such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD). Interestingly, in addition to its roles in the regeneration of the lung, WNT signaling is also involved in the pathology of these diseases. Either activated or repressed WNT signaling is observed in these diseases. As the regenerative capacity of the lung is severely destroyed in these diseases, a proper modulation of WNT signaling activity in these diseases will provide a way to reverse the disease state to normal by enhancing the intrinsic regenerative capacity of the lung.
This work was supported by grants from the Global Research Development Center Program (2016K1A4A3914725) and the National Research Foundation of Korea (2016R1A2B4012956) to J.K.Y. Epifluorescence microscopy was performed using the equipment at the Soonchunhyang Biomedical Research Core Facility.
A.A.R. and J.K.Y. wrote the manuscript and J.K.Y. secured the funding.
The authors have no potential conflicts of interest to disclose.
WNT signaling in various epithelial stem/progenitor cells in mouse lung
Stem cell type | Location | Markers | Contribution to regeneration | Differentiation outcome | WNT signaling role | Reference |
---|---|---|---|---|---|---|
Basal cells (BCs) | Trachea, bronchi (cartilaginous airways) | TRP63, KRT5 (and variably KRT14), NGFR, PDPN (T1α) | Major | Ciliated cells, secretory cells (club and goblet cells) | Increase cell expansion and direct differentiation | (Brechbuhl et al., 2011; Giangreco et al., 2012; Rock et al., 2009) |
Club cells | Trachea, bronchi and bronchioles | SCGB1A1(also known as CCSP and CC10), | Major | Ciliated cells, secretory cells (club and goblet cells), basal cells, | Inhibit differentiation into goblet cells by RYK receptor | (Guha et al., 2017; Lee et al., 2017; Rawlins et al., 2009; Rock et al., 2009; Tata et al., 2013) |
Submucosal gland duct cell (myoepithelial cells [MECs]) | Trachea, bronchi and bronchioles | TRP63, KRT5, KRT14, rich in ACTA2 | Minor | Trachea pithelium | Promotes the transition to basal cell phenotype | (Lynch et al., 2018) |
Neuroendocrine cells (NECs) | Bronchioles | Calcitonin gene-related peptide | Minor | Ciliated cells, club cells | Not reported | (Song et al., 2012; Yao et al., 2018) |
Bronchoalveolar stem cells (BASCs) | Bronchoalveolar duct junction | SCGB1A1, SFTPC | Major | Ciliated cells, club cells, AT1 cells, AT2 cells | Increase cell expansion | (Kim et al., 2005; Liu et al., 2019; Salwig et al., 2019; Zhang et al., 2008) |
Lineage-negative epithelial stem/progenitor (LNEP) | Alveoli | TRP63, KRT5, H2-K1high | Minor | AT1 cells, AT2 cells | Enhance differentiation into AT2 | (Kathiriya et al., 2020; Kumar et al., 2011; Vaughan et al., 2015; Xi et al., 2017; Zuo et al., 2015) |
Distal lung progenitors (SFTPC-ve AT2 cells) | Alveoli | Integrin α6β4 | Minor | Club cells, AT2 cells | Not reported | (Chapman et al., 2011) |
Alveolar type 2 cells (AT2) | Alveoli | SFTPC, AQP3, ABCA3 | Major | AT1 cells | Increase cell expansion and proper differentiation during regeneration | (Barkauskas et al., 2013; Desai et al., 2014; Nabhan et al., 2018; Olajuyin et al., 2019; Tanjore et al., 2013; Zacharias et al., 2018) |
Alveolar type 1 cells (AT1) | Alveoli | AQP5, PDPN (T1α), HOPX | Minor | AT2 cells | Not reported | (Jain et al., 2015) |
Mol. Cells 2020; 43(9): 774-783
Published online September 30, 2020 https://doi.org/10.14348/molcells.2020.0059
Copyright © The Korean Society for Molecular and Cellular Biology.
Ahmed A. Raslan1,2 and Jeong Kyo Yoon1,2,*
1Soonchunhyang Institute of Medi-bio Science and 2Department of Integrated Biomedical Science, Soonchunhyang University, Cheonan 31151, Korea
Correspondence to:*jkyoon@sch.ac.kr
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
The lung has a vital function in gas exchange between the blood and the external atmosphere. It also has a critical role in the immune defense against external pathogens and environmental factors. While the lung is classified as a relatively quiescent organ with little homeostatic turnover, it shows robust regenerative capacity in response to injury, mediated by the resident stem/progenitor cells. During regeneration, regionally distinct epithelial cell populations with specific functions are generated from several different types of stem/progenitor cells localized within four histologically distinguished regions: trachea, bronchi, bronchioles, and alveoli. WNT signaling is one of the key signaling pathways involved in regulating many types of stem/progenitor cells in various organs. In addition to its developmental role in the embryonic and fetal lung, WNT signaling is critical for lung homeostasis and regeneration. In this minireview, we summarize and discuss recent advances in the understanding of the role of WNT signaling in lung regeneration with an emphasis on stem/progenitor cells.
Keywords: β-catenin, lung homeostasis, lung regeneration, lung stem/progenitor cells, WNT signaling
The lung is a structurally complex organ comprising at least 40 different types of cells with diverse functions. It can be sub-divided anatomically into four different regions—trachea, bronchi, bronchioles, and alveoli. To fulfill its different functions, the cellular composition and three-dimensional structure of the lung must be maintained throughout an organism’s lifetime. Under normal conditions, the lung shows a low rate of cellular turnover relative to other organs, such as the skin and intestine, which exhibit rapid kinetics of cellular replacement characteristics (Bowden et al., 1968; Kauffman, 1980; Wansleeben et al., 2013). However, after injury or damage caused by different agents, including infection, toxic compounds, and irradiation, the lung demonstrates a remarkable ability to regenerate the damaged tissue (Hogan et al., 2014; Lee and Rawlins, 2018; Wansleeben et al., 2013). If this regeneration process is not completed successfully, it leads to a disruption in proper lung function accompanied by chronic inflammation, pathologic remodeling, and fibrosis.
Normal cell turnover and regeneration after injury in the lung require regionally- specific epithelial stem/progenitor cells and unique microenvironment or neighboring cells that can crosstalk with each other (Lee and Rawlins, 2018; Rock and Hogan, 2011; Wansleeben et al., 2013). It has been challenging to distinguish the specific properties of lung stem/progenitor cells owing to the histological complexity of the lung and the multiplicity of cell types, besides the slow turnover of the respiratory epithelium. Each region comprises different cell types and the homeostasis in each region is guaranteed by regionally-specific stem/progenitor cells (Ardhanareeswaran and Mirotsou, 2013; Spurlin and Nelson, 2017; Wansleeben et al., 2013). In addition, the regenerative capacity of these lung stem/progenitor cells is not only determined by their intrinsic potential, but also by the microenvironment of their specific niche, which includes the extracellular matrix, stromal and other types of cells, as well as, the many signaling factors derived from them (Hogan et al., 2014; Lee and Rawlins, 2018; Stabler and Morrisey, 2017).
The WNT signaling pathway plays major roles during embryogenesis, tissue homeostasis, and regeneration in many organs (Kahn, 2018; Nusse and Clevers, 2017; Steinhart and Angers, 2018). The functional importance of WNT signaling in the lung is initially demonstrated during lung development, as early as at the stage of lung endoderm specification (Hussain et al., 2017; Majidinia et al., 2018). Recent studies have begun to reveal the roles of WNT signaling in lung homeostasis and regeneration. In this review, we discuss the recent findings on the role of WNT signaling in lung homeostasis and regeneration, while highlighting different lung stem/progenitor cells and the mechanisms that regulate their behavior and function.
Secreted WNT ligands, comprising 19 members, are highly conserved signaling molecules that activate downstream signaling cascades involved in the regulation of gene expression, proliferation, differentiation, adhesion and migration, and polarity in signaling-receiving cells (Kahn, 2018; Nusse and Clevers, 2017; Steinhart and Angers, 2018). The two major WNT signaling pathways are canonical (WNT/β-catenin) and non-canonical pathways (Fig. 1). WNT1 (WNT2, WNT3, WNT3A, and WNT8A) and WNT5A (WNT4, WNT5A, WNT5B, WNT6, WNT7A, and WNT11) class ligands primarily determine the specificity of activation of the canonical and non-canonical pathways, respectively. In the canonical WNT signaling, binding of the WNT1 class ligands to the seven-pass trans-membrane Frizzled receptor family (FZD) and the low-density lipoprotein receptor-related protein family (LRP5/6) initiates a signaling cascade. A hallmark of this pathway is an accumulation of the normally unstable β-catenin protein within the cytoplasm through the inhibition of glycogen synthase kinase 3 beta (GSK-3β) and the subsequent translocation of β-catenin to the nucleus, where it engages in gene transcription (Nusse and Clevers, 2017; Steinhart and Angers, 2018). In contrast, non-canonical WNT signaling is activated by WNT5A class ligands via the FZD receptors, independent of LRP5/6 receptors (Chae and Bothwell, 2018; Steinhart and Angers, 2018). This ligand-receptor binding initiates the activation of WNT/PCP (planar cell polarity) and WNT/Ca2+ pathways. The WNT/PCP pathway activates JNK and Rho-kinase cascades, whereas the WNT/Ca2+ pathway increases intracellular calcium concentration and activates protein kinase C (PKC), calcineurin, and calcium/calmodulin-dependent protein kinase II cascades. In addition to the typical FZD and LRP5/6 receptors, receptor tyrosine kinases (RYK) and retinoic acid-related orphan receptors are also known to function in a cell type-specific manner as cognate receptors for canonical and non-canonical WNT signaling, respectively (Chae and Bothwell, 2018; Nusse and Clevers, 2017). Furthermore, while WNT signaling is positively regulated through secretory agonists, such as R-spondins (RSPOs) and norrin, it is negatively regulated through secretory antagonists, such as dickkopf-1 (DKK1), secreted FZD-related proteins, WNT inhibitory factor 1, and sclerostin (Chae and Bothwell, 2018; Nusse and Clevers, 2017; Raslan and Yoon, 2019).
The epithelial cells of the airway walls represent an effective physical barrier against the external atmosphere and are the first cell type that are damaged by exogenous microorganisms, airborne irritants, and allergens. The epithelial cells exhibit unique stereotypic patterns from the proximal airway axis to the distal airway axis. Multiple studies have shown that diverse types of cells, possessing stem/progenitor-like behavior in response to airway injury, are present throughout the airways (Lee and Rawlins, 2018; Rock et al., 2010; Stabler and Morrisey, 2017; Wansleeben et al., 2013). Both canonical WNT/β-catenin and non-canonical WNT signaling appear to play diverse roles in various stem/progenitor cells and niches cells in a region-dependent manner during regeneration.
A pseudo-stratified columnar epithelium, consisting of basal, secretory (goblet and club [or Clara]), and ciliated cells, lines the trachea and bronchi (Fig. 2) (Hogan et al., 2014; Mercer et al., 1994; Wansleeben et al., 2013). In humans, a majority of the epithelial cells in the trachea are ciliated (Hogan and Tata, 2019; Meyerholz et al., 2018). In contrast, the majority of the epithelial cells in mouse trachea are non-ciliated (Hogan and Tata, 2019; Meyerholz et al., 2018). Moreover, few goblet cells and more club cells exist in mice trachea than in humans (Hogan and Tata, 2019; Meyerholz et al., 2018). Bronchioles contain simple columnar epithelial cells, including club, ciliated, and pulmonary neuroendocrine cells (NECs). NECs are more solitary and scattered in the airways in humans than in mice (Hogan and Tata, 2019; Meyerholz et al., 2018).
Two tracheal cell populations have been identified as stem/progenitor cells in response to injury. The first population is that of basal cells (BCs) that comprise about 30% of the total epithelial population in the trachea. BCs are pluripotent and express the transcription factor TRP63, cytokeratin 5 (KRT5), and the nerve growth factor receptor (NGFR) (Fig. 3, Table 1) (Hogan et al., 2014; Rock et al., 2009; Tata and Rajagopal, 2017). In steady-state and regeneration following damage, BCs can self-renew and differentiate into multiple cell lineages, including ciliated and secretory cells
Independent of BCs, tracheal club cells expressing secretoglobin 1A1 (SCGB1A1, also known as CCSP, CC10, or uteroglobin) display a limited stem/progenitor cell property (Fig. 3, Table 1). While tracheal club cells exhibit a relatively slow rate of self-renewal (Rawlins et al., 2009), upon tracheal injury, they are able to give rise to ciliated cells, but not to other types of cells
In the trachea of adult mice, canonical WNT signaling is activated within BCs following tracheal damage (Brechbuhl et al., 2011; Giangreco et al., 2012) (Fig. 4). In a transgenic mouse model with dominant-negative (N-terminally truncated) lymphoid enhancer-binding factor1 (LEF1) expression driven under the
Unlike trachea, the BC population decreases significantly in the main stem bronchi and is absent in the columnar epithelium of the distal bronchioles in the mouse lung. In contrast, BCs are present throughout all bronchi and bronchioles in the human lung. The goblet cells are very rare in mouse bronchioles airways (Hogan and Tata, 2019; Meyerholz et al., 2018). The epithelia of distal bronchioles comprise club, multi-ciliate, and NECs (Fig. 2) (Hogan and Tata, 2019; Meyerholz et al., 2018). Following naphthalene-induced injury
In the most distant region of the airways, bronchioalveolar stem cells (BASCs) that co-express SCGB1A1 (a club cell marker) and surfactant protein C, SFTPC (an AT2 cell marker) are identified in the bronchioalveolar duct junction. These cells are distinct from UPK3A+ club cells (Fig. 2) (Kim et al., 2005; Salwig et al., 2019). In response to airway injury by naphthalene or influenza virus infection, these BASCs yield diverse populations of differentiated airway cells, including club and ciliated cells
After naphthalene-induced lung injury, WNT ligands (WMT3A, WNT5A, and WNT7B) are upregulated in club cells at an early stage of regeneration (Lee et al., 2017). This indicates the importance of WNT signaling in the regeneration of the bronchiolar epithelium. Surprisingly, club cell-specific deletion of the
Several studies provide additional clues regarding the role of WNT signaling in bronchiole regeneration. Expression of leucine-rich repeat containing G protein-coupled receptor 6 (LGR6), a specific receptor for the WNT positive regulator, RSPOs, and a known marker for many types of stem cells (Barker et al., 2013; Raslan and Yoon, 2019; Schindler et al., 2018) is detected in airway smooth muscle cells (ASMCs), a stromal niche of club cells surrounding the airway epithelia (Lee et al., 2017). These LGR6+ ASMCs play an important role in the regeneration of the airway epithelia. Genetic depletion of LGR6+ ASMCs causes significant defects in airway repair following naphthalene-induced injury in the lung
According to a recent report, club cell-specific deletion of the gene for the canonical WNT receptor,
Alveoli are populated by squamous, gas exchanging AT1 cells and cuboidal, surfactant secreting AT2 cells (Fig. 2). ATI cells are thin, flat cells that cover 95% of the alveolar surface (Fig. 2). They can be identified by the expression of markers such as aquaporin 5 (AQP5), podoplanin (PDPN), RAGE, and HOPX. It has long been considered that AT1 is the most vulnerable target for distal lung injury and aging. AT2 cells express markers such as SFTPC and a lipid transporter, ATP binding cassette A3 transporter (Fig. 3, Table 1). Similar to other epithelial cells in the lung, the alveolar epithelial cells also show a relatively slow rate of cellular turnover (about 28-35 days) under normal conditions. However, after injury by agents such as hyperoxia, NO2, ozone, and bleomycin, alveoli show robust regeneration capacity through extensive cell proliferation and differentiation (Barkauskas et al., 2013; Desai et al., 2014; Lee and Rawlins, 2018; Olajuyin et al., 2019).
Several distinct cell populations are identified as alveolar stem/progenitor cells. Firstly, in the alveolar-injury model induced by bleomycin or influenza virus infection, BASCs localized within the bronchioalveolar region expand and differentiate into AT1 and AT2 cells
Within the alveoli of the normal adult lung, most epithelial cells, including AT2 cells, do not exhibit active canonical WNT signaling. Canonical WNT signaling within AT2 cells gets activated only after bleomycin-induced injury (Flozak et al., 2010; Tanjore et al., 2013). Furthermore, in the bleomycin-injured alveoli, an increase in the number of β-catenin-positive nuclei, as well as, the expression of canonical
In addition to the role in AT2 cell proliferation and survival, both canonical and non-canonical WNT signaling pathways have been shown to regulate AT2 cell differentiation. It is known that the interaction between p300 co-activator and β-catenin increases during the differentiation of AT2 cells into AT1 cells (Rieger et al., 2016). While a nuclear accumulation of β-catenin is induced by canonical WNT signaling, the regulation of p300/β-catenin interaction occurs through the non-canonical WNT5A/PKC signaling axis (Rieger et al., 2016). WNT5A regulates Ser-89 phosphorylation of p300 in a PKC-dependent manner, resulting in enhanced interaction with β-catenin. Inhibition of p300/β-catenin interaction by IQ-1, a specific small-molecule inhibitor, impairs the differentiation of primary AT2 cells. Unlike this study, Frank et al. (2016) showed that canonical WNT signaling inhibits AT2 cell differentiation. It is not yet defined why these contrasting results exist. Nonetheless, both canonical WNT3A and non-canonical WNT5A ligands are derived from the neighboring LGR5+ stromal cells to promote alveolar differentiation of both club and AT2 cells (Lee et al., 2017). Moreover, resident alveolar macrophages also produce WNT ligands, especially WNT4 and WNT16, to stimulate AT2 cell proliferation and improve alveoli regeneration after lung injury induced by
A recent report showed that non-canonical WNT5A and WNT5B ligands repress the proliferation of alveolar epithelial stem/progenitor cells (Wu et al., 2019). It has been demonstrated in
In addition to the alveolar stem/progenitor cells, WNT signaling also plays a critical role in WNT-producing stromal cells, especially the AXIN2+ PDGFRα+ mesenchymal alveolar niche cells during regeneration (Basil et al., 2020; Zepp et al., 2017). These cells are demonstrated to promote the self-renewal and differentiation of AT2 cells during alveoli homeostasis and regeneration by providing different signals such as IL6, FGFs, and BMP antagonists likely induced by canonical WNT signaling. It still remains to be determined whether WNT-producing LGR5+ stromal cells and mesenchymal alveolar niche cells are the same population or not. Given that both
The robust regenerative capacity of the lung after injury reflects the histological complexity of the lung. As the different regions of the lung contain unique populations of stem/progenitor cells and their microenvironment, an efficient and correct regeneration process appears to be imprinted within the identities and differentiation potentials of stem cells and their niche. Both canonical and non-canonical WNT signaling pathways play critical regulatory roles in the function and behavior of the different lung stem cell populations and their niche cells. Especially after lung injury, activated canonical WNT signaling is crucial for proliferation, survival, and differentiation of lung epithelial stem/progenitor cells. In contrast, the functional roles of non-canonical WNT signaling during regeneration are relatively limited and need to be further explored in the future.
Disruption of proper WNT signaling during regeneration results in inefficient regeneration. This insufficient repair and repetitive injury promote impairment of the epithelial-mesenchymal crosstalk for lung homeostasis, which plays a key role in the pathogenesis of chronic lung diseases such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD). Interestingly, in addition to its roles in the regeneration of the lung, WNT signaling is also involved in the pathology of these diseases. Either activated or repressed WNT signaling is observed in these diseases. As the regenerative capacity of the lung is severely destroyed in these diseases, a proper modulation of WNT signaling activity in these diseases will provide a way to reverse the disease state to normal by enhancing the intrinsic regenerative capacity of the lung.
This work was supported by grants from the Global Research Development Center Program (2016K1A4A3914725) and the National Research Foundation of Korea (2016R1A2B4012956) to J.K.Y. Epifluorescence microscopy was performed using the equipment at the Soonchunhyang Biomedical Research Core Facility.
A.A.R. and J.K.Y. wrote the manuscript and J.K.Y. secured the funding.
The authors have no potential conflicts of interest to disclose.
. WNT signaling in various epithelial stem/progenitor cells in mouse lung.
Stem cell type | Location | Markers | Contribution to regeneration | Differentiation outcome | WNT signaling role | Reference |
---|---|---|---|---|---|---|
Basal cells (BCs) | Trachea, bronchi (cartilaginous airways) | TRP63, KRT5 (and variably KRT14), NGFR, PDPN (T1α) | Major | Ciliated cells, secretory cells (club and goblet cells) | Increase cell expansion and direct differentiation | (Brechbuhl et al., 2011; Giangreco et al., 2012; Rock et al., 2009) |
Club cells | Trachea, bronchi and bronchioles | SCGB1A1(also known as CCSP and CC10), | Major | Ciliated cells, secretory cells (club and goblet cells), basal cells, | Inhibit differentiation into goblet cells by RYK receptor | (Guha et al., 2017; Lee et al., 2017; Rawlins et al., 2009; Rock et al., 2009; Tata et al., 2013) |
Submucosal gland duct cell (myoepithelial cells [MECs]) | Trachea, bronchi and bronchioles | TRP63, KRT5, KRT14, rich in ACTA2 | Minor | Trachea pithelium | Promotes the transition to basal cell phenotype | (Lynch et al., 2018) |
Neuroendocrine cells (NECs) | Bronchioles | Calcitonin gene-related peptide | Minor | Ciliated cells, club cells | Not reported | (Song et al., 2012; Yao et al., 2018) |
Bronchoalveolar stem cells (BASCs) | Bronchoalveolar duct junction | SCGB1A1, SFTPC | Major | Ciliated cells, club cells, AT1 cells, AT2 cells | Increase cell expansion | (Kim et al., 2005; Liu et al., 2019; Salwig et al., 2019; Zhang et al., 2008) |
Lineage-negative epithelial stem/progenitor (LNEP) | Alveoli | TRP63, KRT5, H2-K1high | Minor | AT1 cells, AT2 cells | Enhance differentiation into AT2 | (Kathiriya et al., 2020; Kumar et al., 2011; Vaughan et al., 2015; Xi et al., 2017; Zuo et al., 2015) |
Distal lung progenitors (SFTPC-ve AT2 cells) | Alveoli | Integrin α6β4 | Minor | Club cells, AT2 cells | Not reported | (Chapman et al., 2011) |
Alveolar type 2 cells (AT2) | Alveoli | SFTPC, AQP3, ABCA3 | Major | AT1 cells | Increase cell expansion and proper differentiation during regeneration | (Barkauskas et al., 2013; Desai et al., 2014; Nabhan et al., 2018; Olajuyin et al., 2019; Tanjore et al., 2013; Zacharias et al., 2018) |
Alveolar type 1 cells (AT1) | Alveoli | AQP5, PDPN (T1α), HOPX | Minor | AT2 cells | Not reported | (Jain et al., 2015) |
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