Top

Research Article

Split Viewer

Mol. Cells 2002; 13(2): 252-258

Published online April 30, 2002

© The Korean Society for Molecular and Cellular Biology

Identification of a Tolerant Locus on Arabidopsis thaliana to Hypervirulent Beet Curly Top Virus CFH Strain

Seong-Hee Park, Jinkyung Hur, Jongbum Park, Sangseob Lee, Taek-Kyun Lee, Man Chang, Keith R. Davis, Jeongha Kim, Sukchan Lee

Abstract

The infection of hosts by the geminivirus depends on the interactions between host and viral factors for vi-ral DNA replication, viral gene expression, and the movement of virus throughout the hosts. This work reports that a hypervirulent strain of Beet curly top virus (BCTV) is different in its ability to infect several ecotypes of Arabidopsis thaliana. Symptoms appeared on Arabidopsis ecotypes around 7 to 10 d after inocu-lation with BCTV-CFH. Symptoms were more severe in ecotype SKKU including severe leaf curling and development of severely deformed and stunted bolting compared to Col-O as a lab standard ecotype. One ecotype Cen-O was asymptomatic to BCTV-CFH in-fection. Studies of viral DNA replication and virus movement in three excised organs of asymptomatic Cen-O demonstrated that BCTV-CFH could replicate viral DNA and move systemically in this ecotype, sug-gesting that tolerance was due to the blocks of interac-tions between host and viral factors on symptom de-velopment. This asymptomatic phenotype is similar to the mutation of leftward ORFs, especially ORF R2. Genetic analysis of this ecotype Cen-O indicated that tolerance is due to a single recessive locus.

Keywords BCTV, Arabidopsis, Geminivirus, Tolerance.

Article

Research Article

Mol. Cells 2002; 13(2): 252-258

Published online April 30, 2002

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of a Tolerant Locus on Arabidopsis thaliana to Hypervirulent Beet Curly Top Virus CFH Strain

Seong-Hee Park, Jinkyung Hur, Jongbum Park, Sangseob Lee, Taek-Kyun Lee, Man Chang, Keith R. Davis, Jeongha Kim, Sukchan Lee

Abstract

The infection of hosts by the geminivirus depends on the interactions between host and viral factors for vi-ral DNA replication, viral gene expression, and the movement of virus throughout the hosts. This work reports that a hypervirulent strain of Beet curly top virus (BCTV) is different in its ability to infect several ecotypes of Arabidopsis thaliana. Symptoms appeared on Arabidopsis ecotypes around 7 to 10 d after inocu-lation with BCTV-CFH. Symptoms were more severe in ecotype SKKU including severe leaf curling and development of severely deformed and stunted bolting compared to Col-O as a lab standard ecotype. One ecotype Cen-O was asymptomatic to BCTV-CFH in-fection. Studies of viral DNA replication and virus movement in three excised organs of asymptomatic Cen-O demonstrated that BCTV-CFH could replicate viral DNA and move systemically in this ecotype, sug-gesting that tolerance was due to the blocks of interac-tions between host and viral factors on symptom de-velopment. This asymptomatic phenotype is similar to the mutation of leftward ORFs, especially ORF R2. Genetic analysis of this ecotype Cen-O indicated that tolerance is due to a single recessive locus.

Keywords: BCTV, Arabidopsis, Geminivirus, Tolerance.

Mol. Cells
Jul 31, 2022 Vol.45 No.7, pp. 435~512
COVER PICTURE
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into mesodermal lineages like adipogenic, osteogenic, and chondrogenic. Alcian blue-positive extracellular matrix secreted by chondrocytes in the lacuna confirmed the chondrogenic differentiation of MSCs (Bashyal et al., pp. 479-494).

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download