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Mol. Cells 2002; 13(2): 252-258

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Identification of a Tolerant Locus on Arabidopsis thaliana to Hypervirulent Beet Curly Top Virus CFH Strain

Seong-Hee Park, Jinkyung Hur, Jongbum Park, Sangseob Lee, Taek-Kyun Lee, Man Chang, Keith R. Davis, Jeongha Kim, Sukchan Lee

Abstract

The infection of hosts by the geminivirus depends on the interactions between host and viral factors for vi-ral DNA replication, viral gene expression, and the movement of virus throughout the hosts. This work reports that a hypervirulent strain of Beet curly top virus (BCTV) is different in its ability to infect several ecotypes of Arabidopsis thaliana. Symptoms appeared on Arabidopsis ecotypes around 7 to 10 d after inocu-lation with BCTV-CFH. Symptoms were more severe in ecotype SKKU including severe leaf curling and development of severely deformed and stunted bolting compared to Col-O as a lab standard ecotype. One ecotype Cen-O was asymptomatic to BCTV-CFH in-fection. Studies of viral DNA replication and virus movement in three excised organs of asymptomatic Cen-O demonstrated that BCTV-CFH could replicate viral DNA and move systemically in this ecotype, sug-gesting that tolerance was due to the blocks of interac-tions between host and viral factors on symptom de-velopment. This asymptomatic phenotype is similar to the mutation of leftward ORFs, especially ORF R2. Genetic analysis of this ecotype Cen-O indicated that tolerance is due to a single recessive locus.

Keywords BCTV, Arabidopsis, Geminivirus, Tolerance.

Article

Research Article

Mol. Cells 2002; 13(2): 252-258

Published online April 30, 2002

Copyright © The Korean Society for Molecular and Cellular Biology.

Identification of a Tolerant Locus on Arabidopsis thaliana to Hypervirulent Beet Curly Top Virus CFH Strain

Seong-Hee Park, Jinkyung Hur, Jongbum Park, Sangseob Lee, Taek-Kyun Lee, Man Chang, Keith R. Davis, Jeongha Kim, Sukchan Lee

Abstract

The infection of hosts by the geminivirus depends on the interactions between host and viral factors for vi-ral DNA replication, viral gene expression, and the movement of virus throughout the hosts. This work reports that a hypervirulent strain of Beet curly top virus (BCTV) is different in its ability to infect several ecotypes of Arabidopsis thaliana. Symptoms appeared on Arabidopsis ecotypes around 7 to 10 d after inocu-lation with BCTV-CFH. Symptoms were more severe in ecotype SKKU including severe leaf curling and development of severely deformed and stunted bolting compared to Col-O as a lab standard ecotype. One ecotype Cen-O was asymptomatic to BCTV-CFH in-fection. Studies of viral DNA replication and virus movement in three excised organs of asymptomatic Cen-O demonstrated that BCTV-CFH could replicate viral DNA and move systemically in this ecotype, sug-gesting that tolerance was due to the blocks of interac-tions between host and viral factors on symptom de-velopment. This asymptomatic phenotype is similar to the mutation of leftward ORFs, especially ORF R2. Genetic analysis of this ecotype Cen-O indicated that tolerance is due to a single recessive locus.

Keywords: BCTV, Arabidopsis, Geminivirus, Tolerance.

Mol. Cells
Jun 30, 2023 Vol.46 No.6, pp. 329~398
COVER PICTURE
The cellular proteostasis network is adaptively modulated upon cellular stress, thereby protecting cells from proteostasis collapse. Heat shock induces the translocation of misfolded proteins and the chaperone protein HSP70 into nucleolus, where nuclear protein quality control primarily occurs. Nuclear RNA export factor 1 (green), nucleolar protein fibrillarin (red), and nuclei (blue) were visualized in NIH3T3 cells under basal (left) and heat shock (right) conditions (Park et al., pp. 374-386).

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