Mol. Cells 2002; 13(2): 252-258
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
The infection of hosts by the geminivirus depends on the interactions between host and viral factors for vi-ral DNA replication, viral gene expression, and the movement of virus throughout the hosts. This work reports that a hypervirulent strain of Beet curly top virus (BCTV) is different in its ability to infect several ecotypes of Arabidopsis thaliana. Symptoms appeared on Arabidopsis ecotypes around 7 to 10 d after inocu-lation with BCTV-CFH. Symptoms were more severe in ecotype SKKU including severe leaf curling and development of severely deformed and stunted bolting compared to Col-O as a lab standard ecotype. One ecotype Cen-O was asymptomatic to BCTV-CFH in-fection. Studies of viral DNA replication and virus movement in three excised organs of asymptomatic Cen-O demonstrated that BCTV-CFH could replicate viral DNA and move systemically in this ecotype, sug-gesting that tolerance was due to the blocks of interac-tions between host and viral factors on symptom de-velopment. This asymptomatic phenotype is similar to the mutation of leftward ORFs, especially ORF R2. Genetic analysis of this ecotype Cen-O indicated that tolerance is due to a single recessive locus.
Keywords BCTV, Arabidopsis, Geminivirus, Tolerance.
Mol. Cells 2002; 13(2): 252-258
Published online April 30, 2002
Copyright © The Korean Society for Molecular and Cellular Biology.
Seong-Hee Park, Jinkyung Hur, Jongbum Park, Sangseob Lee, Taek-Kyun Lee, Man Chang, Keith R. Davis, Jeongha Kim, Sukchan Lee
The infection of hosts by the geminivirus depends on the interactions between host and viral factors for vi-ral DNA replication, viral gene expression, and the movement of virus throughout the hosts. This work reports that a hypervirulent strain of Beet curly top virus (BCTV) is different in its ability to infect several ecotypes of Arabidopsis thaliana. Symptoms appeared on Arabidopsis ecotypes around 7 to 10 d after inocu-lation with BCTV-CFH. Symptoms were more severe in ecotype SKKU including severe leaf curling and development of severely deformed and stunted bolting compared to Col-O as a lab standard ecotype. One ecotype Cen-O was asymptomatic to BCTV-CFH in-fection. Studies of viral DNA replication and virus movement in three excised organs of asymptomatic Cen-O demonstrated that BCTV-CFH could replicate viral DNA and move systemically in this ecotype, sug-gesting that tolerance was due to the blocks of interac-tions between host and viral factors on symptom de-velopment. This asymptomatic phenotype is similar to the mutation of leftward ORFs, especially ORF R2. Genetic analysis of this ecotype Cen-O indicated that tolerance is due to a single recessive locus.
Keywords: BCTV, Arabidopsis, Geminivirus, Tolerance.
Jingyung Hur, Kenneth Buckley, Sukchan Lee, Keith Davis
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