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Mol. Cells 2003; 15(1): 102-107
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
Identification of Anther-specific Gene Expression from T-DNA Tagging Rice
We have screened a total of 5,500 T-DNA tagging rice lines in which b-glucuronidase (GUS) gene sequence was randomly inserted as a transgene into the plant genome. Histochemical GUS assays were carried out to select the T-DNA tagging rice lines that show its ex-pression in anther. Of the tagging lines screened, three lines were found to express GUS specifically in the anther that is about 0.05%. Microscopic observation of the anther-expressed lines showed specific expres-sion patterns of GUS in the anther, either gameto-phytic or sporophytic specificities. Southern blot analysis revealed that the integration copy number of the transgene was 2.3 in average. The detailed expres-sion patterns were analyzed and discussed.
Keywords Anther-specific Gene, GUS Expression, T-DNA Tagging Rice
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Research Article
Mol. Cells 2003; 15(1): 102-107
Published online February 28, 2003
Copyright © The Korean Society for Molecular and Cellular Biology.
Identification of Anther-specific Gene Expression from T-DNA Tagging Rice
Gothandam K. Muthukalianan, Sanghyun Lee, Hyunsik Yum, Sujin Ku, Minjung Kwun, Hong Gyu Kang, Gynheung An, Yong-Yoon Chung
Abstract
We have screened a total of 5,500 T-DNA tagging rice lines in which b-glucuronidase (GUS) gene sequence was randomly inserted as a transgene into the plant genome. Histochemical GUS assays were carried out to select the T-DNA tagging rice lines that show its ex-pression in anther. Of the tagging lines screened, three lines were found to express GUS specifically in the anther that is about 0.05%. Microscopic observation of the anther-expressed lines showed specific expres-sion patterns of GUS in the anther, either gameto-phytic or sporophytic specificities. Southern blot analysis revealed that the integration copy number of the transgene was 2.3 in average. The detailed expres-sion patterns were analyzed and discussed.
Keywords: Anther-specific Gene, GUS Expression, T-DNA Tagging Rice
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