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Mol. Cells 2003; 15(1): 94-101

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Cell Proliferation Induced by Reactive Oxygen Species Is Mediated via Mitogen-activated Protein Kinase in Chinese Hamster Lung Fibroblast (V79) Cells

Min-Joon Han, Bu-Yeo Kim, Sang-Oh Yoon, An-Sik Chung

Abstract

Reactive oxygen species (ROS) are generated during cellular metabolism or by external factors. Recently, it was learned that ROS can stimulate cellular proliferation and act as a second messenger in cellular signaling. We previously reported that hydroxyl radicals might be the signaling molecules. In the present experiment, phenazine methosulfate (PMS) was used to generate superoxide anion intracellularly. Treatment with 3 mM PMS in V79 cells increased cellular proliferation by 50%. PMS also activated the c-Jun N-terminal kinase (JNK) and p38 MAPK, but not extracellular signal-regulated kinase (ERK) 1/2 and ERK5. In particular, increased proliferation was blocked by pretreatment with SB203580 (an inhibitor of p38 MAPK). At the transcriptional level, the phosphorylation of c-Jun and ATF-2, which are mediated by JNK and p38 MAPK, were also increased by treatment with PMS.

Keywords Cell Proliferation, Superoxide Anion, MAPK, ROS

Article

Research Article

Mol. Cells 2003; 15(1): 94-101

Published online February 28, 2003

Copyright © The Korean Society for Molecular and Cellular Biology.

Cell Proliferation Induced by Reactive Oxygen Species Is Mediated via Mitogen-activated Protein Kinase in Chinese Hamster Lung Fibroblast (V79) Cells

Min-Joon Han, Bu-Yeo Kim, Sang-Oh Yoon, An-Sik Chung

Abstract

Reactive oxygen species (ROS) are generated during cellular metabolism or by external factors. Recently, it was learned that ROS can stimulate cellular proliferation and act as a second messenger in cellular signaling. We previously reported that hydroxyl radicals might be the signaling molecules. In the present experiment, phenazine methosulfate (PMS) was used to generate superoxide anion intracellularly. Treatment with 3 mM PMS in V79 cells increased cellular proliferation by 50%. PMS also activated the c-Jun N-terminal kinase (JNK) and p38 MAPK, but not extracellular signal-regulated kinase (ERK) 1/2 and ERK5. In particular, increased proliferation was blocked by pretreatment with SB203580 (an inhibitor of p38 MAPK). At the transcriptional level, the phosphorylation of c-Jun and ATF-2, which are mediated by JNK and p38 MAPK, were also increased by treatment with PMS.

Keywords: Cell Proliferation, Superoxide Anion, MAPK, ROS

Mol. Cells
Feb 28, 2023 Vol.46 No.2, pp. 69~129
COVER PICTURE
The bulk tissue is a heterogeneous mixture of various cell types, which is depicted as a skein of intertwined threads with diverse colors each of which represents a unique cell type. Single-cell omics analysis untangles efficiently the skein according to the color by providing information of molecules at individual cells and interpretation of such information based on different cell types. The molecules that can be profiled at the individual cell by single-cell omics analysis includes DNA (bottom middle), RNA (bottom right), and protein (bottom left). This special issue reviews single-cell technologies and computational methods that have been developed for the single-cell omics analysis and how they have been applied to improve our understanding of the underlying mechanisms of biological and pathological phenomena at the single-cell level.

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