Top

Research Article

Split Viewer

Mol. Cells 2003; 15(1): 94-101

Published online February 28, 2003

© The Korean Society for Molecular and Cellular Biology

Cell Proliferation Induced by Reactive Oxygen Species Is Mediated via Mitogen-activated Protein Kinase in Chinese Hamster Lung Fibroblast (V79) Cells

Min-Joon Han, Bu-Yeo Kim, Sang-Oh Yoon, An-Sik Chung

Abstract

Reactive oxygen species (ROS) are generated during cellular metabolism or by external factors. Recently, it was learned that ROS can stimulate cellular proliferation and act as a second messenger in cellular signaling. We previously reported that hydroxyl radicals might be the signaling molecules. In the present experiment, phenazine methosulfate (PMS) was used to generate superoxide anion intracellularly. Treatment with 3 mM PMS in V79 cells increased cellular proliferation by 50%. PMS also activated the c-Jun N-terminal kinase (JNK) and p38 MAPK, but not extracellular signal-regulated kinase (ERK) 1/2 and ERK5. In particular, increased proliferation was blocked by pretreatment with SB203580 (an inhibitor of p38 MAPK). At the transcriptional level, the phosphorylation of c-Jun and ATF-2, which are mediated by JNK and p38 MAPK, were also increased by treatment with PMS.

Keywords Cell Proliferation, Superoxide Anion, MAPK, ROS

Article

Research Article

Mol. Cells 2003; 15(1): 94-101

Published online February 28, 2003

Copyright © The Korean Society for Molecular and Cellular Biology.

Cell Proliferation Induced by Reactive Oxygen Species Is Mediated via Mitogen-activated Protein Kinase in Chinese Hamster Lung Fibroblast (V79) Cells

Min-Joon Han, Bu-Yeo Kim, Sang-Oh Yoon, An-Sik Chung

Abstract

Reactive oxygen species (ROS) are generated during cellular metabolism or by external factors. Recently, it was learned that ROS can stimulate cellular proliferation and act as a second messenger in cellular signaling. We previously reported that hydroxyl radicals might be the signaling molecules. In the present experiment, phenazine methosulfate (PMS) was used to generate superoxide anion intracellularly. Treatment with 3 mM PMS in V79 cells increased cellular proliferation by 50%. PMS also activated the c-Jun N-terminal kinase (JNK) and p38 MAPK, but not extracellular signal-regulated kinase (ERK) 1/2 and ERK5. In particular, increased proliferation was blocked by pretreatment with SB203580 (an inhibitor of p38 MAPK). At the transcriptional level, the phosphorylation of c-Jun and ATF-2, which are mediated by JNK and p38 MAPK, were also increased by treatment with PMS.

Keywords: Cell Proliferation, Superoxide Anion, MAPK, ROS

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download