Top

Research Article

Split Viewer

Mol. Cells 2009; 27(1): 67-74

Published online January 31, 2009

https://doi.org/10.1007/s10059-009-0006-2

© The Korean Society for Molecular and Cellular Biology

OsATG10b, an Autophagosome Component, Is Needed for Cell Survival against Oxidative Stresses in Rice

Jun-Hye Shin, Kohki Yoshimoto, Yoshinori Ohsumi, Jong-seong Jeon and Gynheung An

Received: August 15, 2008; Revised: October 20, 2008; Accepted: October 22, 2008

Abstract

Autophagy degrades toxic materials and old organelles, and recycles nutrients in eukaryotic cells. Whereas the studies on autophagy have been reported in other eukaryotic cells, its functioning in plants has not been well elucidated. We analyzed the roles of OsATG10 genes, which are autophagy-related. Two rice ATG10 genes - OsATG10a and OsATG10b - share significant sequence homology (about 75%), and were ubiquitously expressed in all organs examined here. GUS assay indicated that OsATG10b was highly expressed in the mesophyll cells and vascular tissue of younger leaves, but its level of expression decreased in older leaves. We identified T-DNA insertional mutants in that gene. Those osatg10b mutants were sensitive to treatments with high salt and methyl viologen (MV). Monodansylcadaverine-staining experiments showed that the number of autophagosomes was significantly decreased in the mutants compared with the WT. Furthermore, the amount of oxidized proteins increased in MV-treated mutant seedlings. These results demonstrate that OsATG10b plays an important role in the survival of rice cells against oxidative stresses.

Keywords autophagy, ATG10, methyl viologen (MV), monodansylcadaverine (MDC), oxidative stress, rice

Article

Research Article

Mol. Cells 2009; 27(1): 67-74

Published online January 31, 2009 https://doi.org/10.1007/s10059-009-0006-2

Copyright © The Korean Society for Molecular and Cellular Biology.

OsATG10b, an Autophagosome Component, Is Needed for Cell Survival against Oxidative Stresses in Rice

Jun-Hye Shin, Kohki Yoshimoto, Yoshinori Ohsumi, Jong-seong Jeon and Gynheung An

Received: August 15, 2008; Revised: October 20, 2008; Accepted: October 22, 2008

Abstract

Autophagy degrades toxic materials and old organelles, and recycles nutrients in eukaryotic cells. Whereas the studies on autophagy have been reported in other eukaryotic cells, its functioning in plants has not been well elucidated. We analyzed the roles of OsATG10 genes, which are autophagy-related. Two rice ATG10 genes - OsATG10a and OsATG10b - share significant sequence homology (about 75%), and were ubiquitously expressed in all organs examined here. GUS assay indicated that OsATG10b was highly expressed in the mesophyll cells and vascular tissue of younger leaves, but its level of expression decreased in older leaves. We identified T-DNA insertional mutants in that gene. Those osatg10b mutants were sensitive to treatments with high salt and methyl viologen (MV). Monodansylcadaverine-staining experiments showed that the number of autophagosomes was significantly decreased in the mutants compared with the WT. Furthermore, the amount of oxidized proteins increased in MV-treated mutant seedlings. These results demonstrate that OsATG10b plays an important role in the survival of rice cells against oxidative stresses.

Keywords: autophagy, ATG10, methyl viologen (MV), monodansylcadaverine (MDC), oxidative stress, rice

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

Share this article on

  • line
  • mail

Related articles in Mol. Cells

Molecules and Cells

eISSN 0219-1032
qr-code Download