Communication
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Mol. Cells 2003; 16(3): 397-401
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
Cloning of the Correct Full Length cDNA of NF-kB-repressing Factor
NF-kB-repression factor (NRF) is a nuclear inhibitor of NF-kB proteins that can silence the IFNb promoter. Since NRF was cloned in 1999, in-depth studies have been conducted on the biological functions of this con-stitutive repressor of NF-kB proteins. During large-scale sequencing of a human fetal brain cDNA library we isolated a novel human cDNA that proved to be a correct full-length NRF cDNA. The deduced protein contains 690 aa, and has a G-patch and an R3H do-main at its C-terminus. The size of the protein is con-sistent with its counterparts in mouse and rat. There is considerable evidence that there are some mistakes in the NRF cDNA sequence reported by Nourbakhsh. Here we report the correct, full-length cDNA and pro-tein sequences of NRF. Full-length NRF cDNA is 3247 bp long, contains three exons and maps to human chromosome Xq24. RT-PCR shows that NRF is widely expressed in human tissues.
Keywords G-patch; NRF; R3H; RT-PCR
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Communication
Mol. Cells 2003; 16(3): 397-401
Published online December 31, 2003
Copyright © The Korean Society for Molecular and Cellular Biology.
Cloning of the Correct Full Length cDNA of NF-kB-repressing Factor
Dai Jianfeng, Jin Feng, Ji Chaoneng, Zheng Zhongshou, Gu Shaohua, Wu Qihan, Wang Liu, Yin Gang, Xie Yi, Yumin Mao
Abstract
NF-kB-repression factor (NRF) is a nuclear inhibitor of NF-kB proteins that can silence the IFNb promoter. Since NRF was cloned in 1999, in-depth studies have been conducted on the biological functions of this con-stitutive repressor of NF-kB proteins. During large-scale sequencing of a human fetal brain cDNA library we isolated a novel human cDNA that proved to be a correct full-length NRF cDNA. The deduced protein contains 690 aa, and has a G-patch and an R3H do-main at its C-terminus. The size of the protein is con-sistent with its counterparts in mouse and rat. There is considerable evidence that there are some mistakes in the NRF cDNA sequence reported by Nourbakhsh. Here we report the correct, full-length cDNA and pro-tein sequences of NRF. Full-length NRF cDNA is 3247 bp long, contains three exons and maps to human chromosome Xq24. RT-PCR shows that NRF is widely expressed in human tissues.
Keywords: G-patch, NRF, R3H, RT-PCR
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