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Mol. Cells 2004; 17(1): 132-139

Published online February 29, 2004

© The Korean Society for Molecular and Cellular Biology

Vitamin C and Vitamin E Protect the Rat Testes from Cadmium-induced Reactive Oxygen Species

Ronojoy Sen Gupta, Enakshi Sen Gupta, Bijaya Kumar Dhakal, Ashoke Ranjan Thakur, Joohong Ahnn

Abstract

Cadmium is an environmental and industrial pollutant that affects the male reproductive system of humans and animals. However, the mechanism of its adverse effect on Leydig cell steroidogenesis remains unknown. The present study points to the possible involvement of oxidative stress in the suppression of steroidogenesis. Cadmium administration caused an increase in reactive oxygen species (ROS) by elevating testicular malondialdehyde (MDA) and decreasing the activities of testicular antioxidant enzymes such as glutathione peroxidase and superoxide dismutase. The mRNA of Steroid Acute Regulatory (StAR) protein was substantially reduced. The activities of testicular D5-3b and 17-b-hydroxysteroid dehydrogenases (HSD) as well as serum testosterone level were also lowered, suggesting that cadmium-induced ROS inhibit testicular steroidogenesis. Supplementation with vitamin C (VC) and or vitamin E (VE) reduced testicular ROS and restored normal testicular function in Cd-exposed rats. We conclude that VC and VE prevent oxidative stress and play vital roles in co-regulating StAR gene expression and steroid production in cadmium-exposed rats.

Keywords Cadmium; ROS; Testes; Vitamin C; Vitamin E

Article

Communication

Mol. Cells 2004; 17(1): 132-139

Published online February 29, 2004

Copyright © The Korean Society for Molecular and Cellular Biology.

Vitamin C and Vitamin E Protect the Rat Testes from Cadmium-induced Reactive Oxygen Species

Ronojoy Sen Gupta, Enakshi Sen Gupta, Bijaya Kumar Dhakal, Ashoke Ranjan Thakur, Joohong Ahnn

Abstract

Cadmium is an environmental and industrial pollutant that affects the male reproductive system of humans and animals. However, the mechanism of its adverse effect on Leydig cell steroidogenesis remains unknown. The present study points to the possible involvement of oxidative stress in the suppression of steroidogenesis. Cadmium administration caused an increase in reactive oxygen species (ROS) by elevating testicular malondialdehyde (MDA) and decreasing the activities of testicular antioxidant enzymes such as glutathione peroxidase and superoxide dismutase. The mRNA of Steroid Acute Regulatory (StAR) protein was substantially reduced. The activities of testicular D5-3b and 17-b-hydroxysteroid dehydrogenases (HSD) as well as serum testosterone level were also lowered, suggesting that cadmium-induced ROS inhibit testicular steroidogenesis. Supplementation with vitamin C (VC) and or vitamin E (VE) reduced testicular ROS and restored normal testicular function in Cd-exposed rats. We conclude that VC and VE prevent oxidative stress and play vital roles in co-regulating StAR gene expression and steroid production in cadmium-exposed rats.

Keywords: Cadmium, ROS, Testes, Vitamin C, Vitamin E

Mol. Cells
Nov 30, 2022 Vol.45 No.11, pp. 763~867
COVER PICTURE
Naive (cyan) and axotomized (magenta) retinal ganglion cell axons in Xenopus tropicalis (Choi et al., pp. 846-854).

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