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Mol. Cells 2004; 17(2): 353-359

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Intracellular Delivery of p53 Fused to the Basic Domain of HIV-1 Tat

Jiyoon Ryu, Hak Joo Lee, Kyeong-Ae Kim, Jae Yong Lee, Kil Soo Lee, Jinseu Park, Soo Young Choi

Abstract

p53 is a potent tumor suppressor inactivated in many cancers. In this study, the membrane permeability of the HIV-1 Tat basic domain was exploited to introduce functional p53 into cancer cells. We expressed and purified a p53 fusion protein with the HIV-1 Tat basic domain at its N terminus (Tat-p53), and examined its transduction profile and biological activity in cancer cells. Tat-p53 was efficiently delivered to both the cytoplasm and nucleus of cells, and was transcriptionally active, as judged by the level of p21/WAF1 protein and of p21 promoter activity. Transduction of cells with Tat-p53 resulted in apoptotic cell death in both p53 positive and negative human tumor cell lines. These results suggest that Tat-p53 could be useful in cancer therapy.

Keywords Apoptosis; Cancer Therapy; Tat; Transduction; Tumor Suppressor

Article

Research Article

Mol. Cells 2004; 17(2): 353-359

Published online April 30, 2004

Copyright © The Korean Society for Molecular and Cellular Biology.

Intracellular Delivery of p53 Fused to the Basic Domain of HIV-1 Tat

Jiyoon Ryu, Hak Joo Lee, Kyeong-Ae Kim, Jae Yong Lee, Kil Soo Lee, Jinseu Park, Soo Young Choi

Abstract

p53 is a potent tumor suppressor inactivated in many cancers. In this study, the membrane permeability of the HIV-1 Tat basic domain was exploited to introduce functional p53 into cancer cells. We expressed and purified a p53 fusion protein with the HIV-1 Tat basic domain at its N terminus (Tat-p53), and examined its transduction profile and biological activity in cancer cells. Tat-p53 was efficiently delivered to both the cytoplasm and nucleus of cells, and was transcriptionally active, as judged by the level of p21/WAF1 protein and of p21 promoter activity. Transduction of cells with Tat-p53 resulted in apoptotic cell death in both p53 positive and negative human tumor cell lines. These results suggest that Tat-p53 could be useful in cancer therapy.

Keywords: Apoptosis, Cancer Therapy, Tat, Transduction, Tumor Suppressor

Mol. Cells
Nov 30, 2023 Vol.46 No.11, pp. 655~725
COVER PICTURE
Kim et al. (pp. 710-724) demonstrated that a pathogen-derived Ralstonia pseudosolanacearum type III effector RipL delays flowering time and enhances susceptibility to bacterial infection in Arabidopsis thaliana. Shown is the RipL-expressing Arabidopsis plant, which displays general dampening of the transcriptional program during pathogen infection, grown in long-day conditions.

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Molecules and Cells

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