Mol. Cells 2005; 20(2): 219-227
Published online January 1, 1970
© The Korean Society for Molecular and Cellular Biology
Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.
Keywords Agtpbp1; Cerebellum; Gene Expression; Microarray; pcd; Purkinje Cell Degeneration
Mol. Cells 2005; 20(2): 219-227
Published online October 31, 2005
Copyright © The Korean Society for Molecular and Cellular Biology.
Rui Xiao, Youngsook Park, Vijaya R. Dirisala, Ya-Ping Zhang, Sang June Um, Hoon Taek Lee, Chankyu Park
Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.
Keywords: Agtpbp1, Cerebellum, Gene Expression, Microarray, pcd, Purkinje Cell Degeneration