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Mol. Cells 2005; 20(3): 364-370

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Sulforaphane Inhibits Osteoclastogenesis by Inhibiting Nuclear Factor-κB

Soo-Jin Kim, So-Young Kang, Hyun-Hee Shin, Hye-Seon Choi

Abstract

We show that sulforaphane inhibits osteoclastogenesis in the presence of macrophage colony-stimulating factor (M-CSF) and receptor for activation of nuclear factor-kB ligand (RANKL) in osteoclast (OC) precursors. Sulforaphane, an aliphatic isothiocyanate, is a known cancer chemo-preventative agent with anti-oxidative properties. Nuclear factor-kB (NF-kB) is a critical transcription factor in RANKL-induced osteoclastogenesis, and electrophoretic mobility shift assays (EMSAs) and assay of NF-kB-mediated secreted alkaline phosphatase (SEAP) revealed that sulforaphane selectively inhibited NF-κB activation induced by RANKL. Inhibition may involve interaction of sulforaphane with thiol groups, since it was prevented by reducing agents.

Keywords Bone Marrow-derived Macrophages; NF-κB; Osteoclastogenesis; RANKL; Sulforaphane; Thiol

Article

Research Article

Mol. Cells 2005; 20(3): 364-370

Published online December 31, 2005

Copyright © The Korean Society for Molecular and Cellular Biology.

Sulforaphane Inhibits Osteoclastogenesis by Inhibiting Nuclear Factor-κB

Soo-Jin Kim, So-Young Kang, Hyun-Hee Shin, Hye-Seon Choi

Abstract

We show that sulforaphane inhibits osteoclastogenesis in the presence of macrophage colony-stimulating factor (M-CSF) and receptor for activation of nuclear factor-kB ligand (RANKL) in osteoclast (OC) precursors. Sulforaphane, an aliphatic isothiocyanate, is a known cancer chemo-preventative agent with anti-oxidative properties. Nuclear factor-kB (NF-kB) is a critical transcription factor in RANKL-induced osteoclastogenesis, and electrophoretic mobility shift assays (EMSAs) and assay of NF-kB-mediated secreted alkaline phosphatase (SEAP) revealed that sulforaphane selectively inhibited NF-κB activation induced by RANKL. Inhibition may involve interaction of sulforaphane with thiol groups, since it was prevented by reducing agents.

Keywords: Bone Marrow-derived Macrophages, NF-κB, Osteoclastogenesis, RANKL, Sulforaphane, Thiol

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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