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Mol. Cells 2006; 21(2): 206-212

Published online January 1, 1970

© The Korean Society for Molecular and Cellular Biology

Myogenic Differentiation of p53- and Rb-deficient Immortalized and Transformed Bovine Fibroblasts in Response to MyoD

Xun Jin, Joong-Seub Lee, Sungwook Kwak, Ji-Eun Jung, Tae-Kyung Kim, Chenxiong Xu, Zhongshan Hong, Zhehu Li, Sun-Myoung Kim, Kwang Youn Whang, Ki-Chang Hong, Seungkwon You, Yun-Jaie Choi, Hyunggee Kim

Abstract

We have established in culture a spontaneously immortalized bovine embryonic fibroblast (BEF) cell line that has lost p53 and p16INK4a functions. MyoD is a muscle-specific regulator capable of inducing myogenesis in a number of cell types. When the BEF cells were transduced with MyoD they differentiated efficiently to desmin-positive myofibers in the presence of 2% horse serum and 1.7 nM insulin. The myogenic differentiation of this cell line was more rapid and obvious than that of C2C12 cells, as judged by morphological changes and expression of various muscle regulatory factors. To confirm that lack of the p53 and p16INK4a pathway does not prevent MyoD-mediated myogenesis, we established a cell line transformed with SV40LT (BEFV) and introduced MyoD into it. In the presence of 2% horse serum and 1.7 nM insulin, the MyoD-transduced BEFV cells differentiated like the MyoD-transduced BEFS cells, and displayed a similar pattern of expression of muscle regulatory proteins. Taken together, our results indicate that MyoD overexpression overcomes the defect in muscle differentiation associated with immortalization and cell transformation caused by the loss of p53 and Rb functions.

Keywords BEF; Muscle Differentiation; MyoD; p53; Rb; SV40LT.

Article

Research Article

Mol. Cells 2006; 21(2): 206-212

Published online April 30, 2006

Copyright © The Korean Society for Molecular and Cellular Biology.

Myogenic Differentiation of p53- and Rb-deficient Immortalized and Transformed Bovine Fibroblasts in Response to MyoD

Xun Jin, Joong-Seub Lee, Sungwook Kwak, Ji-Eun Jung, Tae-Kyung Kim, Chenxiong Xu, Zhongshan Hong, Zhehu Li, Sun-Myoung Kim, Kwang Youn Whang, Ki-Chang Hong, Seungkwon You, Yun-Jaie Choi, Hyunggee Kim

Abstract

We have established in culture a spontaneously immortalized bovine embryonic fibroblast (BEF) cell line that has lost p53 and p16INK4a functions. MyoD is a muscle-specific regulator capable of inducing myogenesis in a number of cell types. When the BEF cells were transduced with MyoD they differentiated efficiently to desmin-positive myofibers in the presence of 2% horse serum and 1.7 nM insulin. The myogenic differentiation of this cell line was more rapid and obvious than that of C2C12 cells, as judged by morphological changes and expression of various muscle regulatory factors. To confirm that lack of the p53 and p16INK4a pathway does not prevent MyoD-mediated myogenesis, we established a cell line transformed with SV40LT (BEFV) and introduced MyoD into it. In the presence of 2% horse serum and 1.7 nM insulin, the MyoD-transduced BEFV cells differentiated like the MyoD-transduced BEFS cells, and displayed a similar pattern of expression of muscle regulatory proteins. Taken together, our results indicate that MyoD overexpression overcomes the defect in muscle differentiation associated with immortalization and cell transformation caused by the loss of p53 and Rb functions.

Keywords: BEF, Muscle Differentiation, MyoD, p53, Rb, SV40LT.

Mol. Cells
Jan 31, 2023 Vol.46 No.1, pp. 1~67
COVER PICTURE
RNAs form diverse shapes and play multiple functions as central molecules of gene expression. In this special issue on RNA, seven minireviews illustrate how basic concepts and recent RNA biology findings are transformed into new and exciting RNA therapeutics.

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